Journal of Applied Phycology

, Volume 26, Issue 1, pp 273–278 | Cite as

Biochemical characterization of some cyanobacterial strains from salt marshes of the Venice Lagoon

  • A. A. Sfriso
  • D. Marchetto
  • M. Gallo
  • F. Baldi


Three different strains of filamentous cyanobacteria, Tychonema, Limnothrix, and Pseudoanabaena, were selected among the fastest growing taxa collected in the salt marshes of Venice Lagoon and were grown in laboratory for growth rate determination and biochemical characterization of chlorophyll-a, total proteins, total carbohydrates, and exopolysaccharides. Experiments were carried out both in liquid medium and two different substrates: artificial plant protection fabric and ground indigenous shells. Cyanobacterial behavior was recorded to better understand colonization of natural and new artificial marshes.


Cyanobacteria Salt marshes Growth rates Polysaccharides Venice Lagoon 


  1. Anagnostidis K, Komarek J (1985) Modern approach to the classification system of cyanophytes. Arch Hydrobiol 71:291–302Google Scholar
  2. Bender J, Phillips P (2004) Microbial mats for multiple applications in aquaculture and bioremediation. Bioresour Technol 94:229–238PubMedCrossRefGoogle Scholar
  3. Booth WE (1941) Algae as pioneers in plant succession and their importance in erosion. Ecology 221:38–46CrossRefGoogle Scholar
  4. Bradford MM (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 81:481–484Google Scholar
  5. Decho WA, Lopez GR (1993) Exopolymer microenvironments of microbial flora: multiple and interactive effects on trophic relationships. Limnol Oceanogr 388:1633–1645CrossRefGoogle Scholar
  6. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem 28:350–356Google Scholar
  7. Ferris MJ, Hirsch CF (1991) Method for isolation and purification of cyanobacteria. Appl Environ Microbiol 575:1448–1452Google Scholar
  8. Klock JH, Wieland A, Seifert R, Michaelis W (2007) Extracellular polymeric substances (EPS) from cyanobacterial mats: characterization and isolation method optimization. Mar Biol 152:1077–1085CrossRefGoogle Scholar
  9. Lorenzen CJ (1967) Determination of chlorophyll and pheopigments: spectrophotometric equations. Limnol Oceanogr 12:343–346CrossRefGoogle Scholar
  10. Nunn BL, Keil RG (2006) A comparison of non-hydrolytic methods for extracting amino acids and proteins from coastal marine sediments. Mar Chem 98:31–42CrossRefGoogle Scholar
  11. Prochnow J, Schweim C, Koengeter J (2002) Simulation of biogenic sediment stabilization by heterotrophic bacteria in annular flume. Proc Mar Sci 5:393–407CrossRefGoogle Scholar
  12. Rao P, Pattabiraman TN (1989) Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentoses. Anal Biochem 1811:18–22CrossRefGoogle Scholar
  13. Rippka R (1988) Isolation and purification of cyanobacteria. Methods Enzymol 167:3–28PubMedCrossRefGoogle Scholar
  14. Sarretta A, Pillon S, Molinaroli E, Guerzoni S, Fontolan G (2009) Sediment budget in the Lagoon of Venice, Italy. Cont Shelf Res 30:934–949CrossRefGoogle Scholar
  15. Schwarz R, Forchhammer K (2005) Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses. Microbiology 151:2503–2514PubMedCrossRefGoogle Scholar
  16. Stainer RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue–green algae (order Chroococcales). Bacteriol Rev 32:171–205Google Scholar
  17. Stal LJ (2010) Microphytobenthos as a biogeomorphological force in intertidal sediment stabilization. Ecol Eng 362:236–245CrossRefGoogle Scholar
  18. Underwood GJC, Paterson DM, Parkes RJ (1995) The measurements of microbial carbohydrates exopolymers from intertidal sediments. Limnol Oceanogr 40:1243–1253CrossRefGoogle Scholar
  19. Underwood GJC (1997) Microalgal colonization in a saltmarsh restoration scheme. Estuar Coast Shelf Sci 44:471–481CrossRefGoogle Scholar
  20. Waterbury JB, Stainer RY (1981) Isolation and growth of cyanobacteria from 214 marine and hypersaline environments. In: Starr HP, Stulp H, Truper HG, Balows A, Schleeper HG (eds) The prokaryotes: a handbook on habitats, isolation, and identification of bacteria, vol 1. Springer, Berlin, pp 221–223CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • A. A. Sfriso
    • 1
  • D. Marchetto
    • 1
  • M. Gallo
    • 1
  • F. Baldi
    • 1
  1. 1.Dipartimento di Scienze Molecolari e NanosistemiCà Foscari University of VeniceVeniceItaly

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