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Extraction optimization and analysis of monosaccharide composition of fucoidan from Saccharina japonica by capillary zone electrophoresis

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Abstract

To optimize the extraction process of fucoidan from Saccharina japonica, an orthogonal test with four factors and three levels was designed in the present experiment. The results showed that the highest extraction yield was obtained under the following conditions: extraction temperature, 90 °C; extraction time, 1.5 h; number of extractions, 3; and pH of the extraction solution, 2.5. Furthermore, a capillary zone electrophoresis method using a UV detector was established for the analysis of the monosaccharide composition of fucoidan via 1-phenyl-3-methyl-5-pyrazolone derivatization. The results showed that fucoidans found in the three different samples all consisted of xylose, rhamnose, fucose, mannose, galactose, and glucuronic acid, while the mole ratios of monosaccharide components revealed differently. Moreover, fucose and galactose were found to be the most abundant monosaccharides in the samples. The above results could be further used for in-depth study on the mechanism of biological activity of fucoidan from S. japonica.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (no. 20905019), Natural Science Foundation of Hebei Province (no. B2010000209), and Natural Science Foundation of Hebei University (no. 2007-111).

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Authors and Affiliations

  1. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmacy, Hebei University, No.180, Wusi East Road, Baoding, 071002, China

    Huaizhong Guo, Fangli Liu, Guiying Jia, Weiquan Zhang & Fang Wu

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  1. Huaizhong Guo
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  2. Fangli Liu
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  3. Guiying Jia
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  4. Weiquan Zhang
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  5. Fang Wu
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Correspondence to Huaizhong Guo.

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Guo, H., Liu, F., Jia, G. et al. Extraction optimization and analysis of monosaccharide composition of fucoidan from Saccharina japonica by capillary zone electrophoresis. J Appl Phycol 25, 1903–1908 (2013). https://doi.org/10.1007/s10811-013-0024-5

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  • DOI: https://doi.org/10.1007/s10811-013-0024-5

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