Optimization of flocculation conditions for Botryococcus braunii using response surface methodology
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Biodiesel from microalgae is recognized as a desirable, renewable biofuel to replace petroleum-derived transport fuels. However, the efficient harvesting of microalgae is a major hurdle for commercialization. Therefore, the development of a cost-effective harvesting method is essential to reduce production cost. A partial factorial design was used to screen the main factors involved, which were the concentration of FeCl3, the bioflocculant, and the time of slow mixing. Response surface methodology (RSM) was used to further investigate the optimal conditions for these factors on flocculation of Botryococcus braunii. Analysis of variance and other relevant tests confirmed the validity of the suggested model. The optimal conditions inferred from the obtained equation were 0.79 mM FeCl3, 0.58 % (v/v) bioflocculant, and 180 sec of slow mixing for 1.1 g DCW L−1 of B. braunii. The flocculating activity under these conditions was 90.6 %. By using RSM, the optimal conditions for flocculation of B. braunii could be reached more quickly and efficiently.
KeywordsBiodiesel Botryococcus braunii Flocculation Harvest Response surface methodology
This research was supported by the Advanced Biomass R&D Center (ABC) funded by the Korean Ministry of Education, Science & Technology (ABC-2010-0029723) and by a grant from the Carbon Dioxide Reduction & Sequestration Research Center, a 21st Century Frontier Program funded by the Korean Ministry of Education, Science & Technology.