Journal of Applied Phycology

, Volume 25, Issue 4, pp 1055–1064 | Cite as

Algicidal effects on Heterosigma akashiwo and Chattonella marina (Raphidophyceae), and toxic effects on natural plankton assemblages by a thiazolidinedione derivative TD49 in a microcosm

  • Seung Ho Baek
  • Min-Chul Jang
  • Moonho Son
  • Si Wouk Kim
  • Hoon Cho
  • Young Ok Kim


The algicidal effects of the thiazolidinedione derivative TD49 on Heterosigma akashiwo and Chattonella marina (Raphidophyceae) were assessed, and the response of the planktonic community and environment to the algicide was evaluated in a microcosm, quantifying 12 L. The abundance of over 80 % of H. akashiwo and C. marina declined in a day significantly in microcosms to which TD49 was added (final concentration 2 μM), and this was correlated with an abrupt decline in the culture pH. The number of protists (i.e., ciliates) other than H. akashiwo and C. marina gradually increased with time in the TD49 treatments, implying that the decline in numbers of H. akashiwo and C. marina cells resulting from TD49 treatment was a major factor in the growth of the other organisms. However, TD49 may be toxic to aquatic zooplankton communities, even though it is a highly selective algicide for harmful algae bloom species. The study indicates that TD49 is an effective agent for the control for H. akashiwo and C. marina blooms in enclosed and eutrophic water bodies.


Thiazolidinedione (TD49) Harmful algal bloom species Chemical control Heterosigma akashiwo Chattonella marina Raphidophyceae 



This research was supported by the Pioneer Research Center Program through the National Research Program of Korea funded by the Ministry of Education, Science and Technology (grant no. M1071118001-08M1118-00110) and KIOST project (PE98745).


  1. Anderson DM (1997) Turning back the harmful red tide. Nature 388:513–514CrossRefGoogle Scholar
  2. Baek SH, Jang MC, Son MH, Joo HM, Cho H, Kim YO (2012a) Assessment of new algicidal thiazolidinedione (TD49) for the control of marine red tide organisms. “The Sea” J Korean Soc Oceanogr 17:9–15 (In Korean)Google Scholar
  3. Baek SH, Jang MC, Son MH, Joo HM, Cho H, Kim YO (2012b) Algicidal effects of a newly developed thiazolidinedione derivative TD49, on dinoflagellate Akashiwo sanguinea. Ocean Polar Res 34:1–11 (In Korean)CrossRefGoogle Scholar
  4. Baek SH, Jung SW, Jang MC, Hyun B, Shin K (2012c) Survival potential of autotrophic phytoplankton species collected from ballast water in international commercial ships. NZ J Mar Fresh Res 46:125–136CrossRefGoogle Scholar
  5. Chan FH, Anderson C, Boustead NC (1990) First record of a Heterosigma (Raphidophyceae) bloom with associated mortality of cage-reared salmon in Big Glory Bay, New Zealand. NZ J Mar Fresh Res 24:461–469CrossRefGoogle Scholar
  6. Han HK, Kim YM, Lim SJ, Hong SS, Jung SG, Cho H, Lee WJ, Jin ES (2011) Enhanced efficacy of TD53, a novel algicidal agent, against the harmful algae via the liposomal delivery system. Int J Pharmaceutics 405:137–141CrossRefGoogle Scholar
  7. Honjo T (1994) The biology and predication of representative red tides associated with fish kills in Japan. Rev Fish Sci 2:225–253CrossRefGoogle Scholar
  8. Imai I, Ishida Y, Hata Y (1993) Killing of marine phytoplankton by a gliding bacterium Cytophaga sp., isolated from the coastal sea of Japan. Mar Biol 116:527–532CrossRefGoogle Scholar
  9. Imai I, Yanaguchi M, Hori Y (2006) Eutrophication and occurrences of harmful algal blooms in the Seto Inland Sea, Japan. Plankton Benthos Res 1:71–84CrossRefGoogle Scholar
  10. Jeong JH, Jin HJ, Sohn CH, Suh KH, Hong YK (2000) Algicidal activity of the seaweed Corallina pilulifera against red tide microalgae. J Appl Phycol 12:37–43CrossRefGoogle Scholar
  11. Jeong S-Y, Ishida K, Ito Y, Okada S, Murakami M (2003) Bacillamide, a novel algicide from the marine bacterium, Bacillus sp. SY-1, against the harmful dinoflagellate, Cochlodinium polykrikoides. Tet Lett 44:8005–8007CrossRefGoogle Scholar
  12. Jung SW, Kang YH, Katano T, Kim BH, Cho SY, Lee JH, Kim YO, Han MS (2010) Test addition of Pseudomonas fluorescens HYK0210-SK09 to mitigate blooms of the diatom Stephanodiscus hantzschii in small- and large-scale mesocosm. J Appl Phycol 22:409–419CrossRefGoogle Scholar
  13. Kang YH, Kim BR, Choi HJ, Seo JG, Kim BH, Han MS (2007) Enhancement of algicidal activity by immobilization of algicidal bacteria antagonistic to Stephanodiscus hantzschii (Bacillariophyceae). J Appl Microbiol 20:375–386Google Scholar
  14. Kim YM, Wu Y, Duong TU, Ghodake GS, Kim SW, Jin ES, Cho H (2010) Thiazolidinediones as a novel class of algicides against red tide harmful algal species. Appl Biochem Biotech 162:2273–2283CrossRefGoogle Scholar
  15. Kim YM, Wu YW, Duong TU, Jung SG, Kim SW, Cho H, Jin ES (2012) Algicidal activity of thiazolidinedione derivatives against harmful algal blooming species. Mar Biotech 14:312–322CrossRefGoogle Scholar
  16. Kim JS, Kim JC, Lee S, Lee BH, Cho KY (2006) Biological activity of l-2-azetidinecarboxylic acid, isolated from Polygonatum odoratum var. pluriflorum, against several algae. Aquat Bot 85:1–6CrossRefGoogle Scholar
  17. Kwon SK, Park YK, Kim JF (2010) Genome-wide screening and identification of factors affecting the biosynthesis of prodigiosin by Hahella chejuensis, using Escherichia coli as a surrogate host. Appl Environ Microbiol 76:1661–1668PubMedCrossRefGoogle Scholar
  18. Lam AKY, Prepas EE, Spink D, Hrudey SE (1995) Chemical control of hepatotoxic phytoplankton: implications for human health. Water Res 29:1845–1854CrossRefGoogle Scholar
  19. Lee HK, Cho H, Han HK (2010) Improved dissolution of poorly water soluble TD49, a novel algicidal agent, via the preparation of solid dispersion. J Pharm Invest 40:181–185CrossRefGoogle Scholar
  20. Lehman JM, Moore LB, Smith-Oliver TA, Wilkinson WO, Willson TM, Kliewer SA (1995) An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor (PPAR). J Biol Chem 270:12953–12956CrossRefGoogle Scholar
  21. Li FM, Hu HY (2005) Isolation and characterization of a novel antialgal allelochemical from Phragmites communis. Appl Environ Microbiol 71:6545–6553PubMedCrossRefGoogle Scholar
  22. Liu J, Zhang H, Yang W, Gao J, Ke Q (2004) Studies on biquaternary ammonium salt algaecide for removing red tide. Mar Sci Bull 6:60–65CrossRefGoogle Scholar
  23. Nagai K, Matsuyama Y, Uchida Y, Yamaguchi M, Ishimura M, Nishimura A, Akamatsu S, Honjo T (1996) Toxicity and LD50 levels of the red tide dinoflagellate Heterocapsa circularisquama on juvenile pearl oysters. Aquaculture 144:149–154CrossRefGoogle Scholar
  24. Nagasaki K, Yamaguchi M (1998) Intra-species host specificity of HaV (Heterosigma akashiwo virus) clones. Aquat Microbiol Ecol 14:109–112CrossRefGoogle Scholar
  25. Park JS, Kim HG, Lee SG (1989) Studies on red tide phenomena in Korea coastal water. In: Okaichi T, Anderson DM, Nemoto T (eds) Red tides: biology, environmental science, and toxicology. Elsevier, New York, pp 37–40Google Scholar
  26. Porsbring T, Blanck H, Tjellström H, Backhaus T (2009) Toxicity of the pharmaceutical clotrimazole to marine microalgal communities. Aquat Toxicol 91:203–211PubMedCrossRefGoogle Scholar
  27. Russell JB, Dombrowski DB (1980) Effect of pH on the efficiency of growth by pure culture of rumen bacteria in continuous culture. Appl Environ Microbiol 39:604–610PubMedGoogle Scholar
  28. Schrader KK, Nanayakkara NPD, Tucker CS, Rimando AM, Ganzera M, Schaneberg BT (2003) Novel derivatives of 9,10-anthraquinone are selective algicides against the musty-odor cyanobacterium Oscillatoria perornata. Appl Environ Microbiol 69:5319–5327PubMedCrossRefGoogle Scholar
  29. Shimada M, Murakami TH, Imahayashi T, Ozaki HS, Toyoshima T, Okaichi T (1983) Effects of sea bloom, Chattonella antiqua, on gill primary lamellae of the young yellowtail Seriola quinqueradiata. Act Histochem Cytochem 16:232–244CrossRefGoogle Scholar
  30. Smayda T (1998) Ecophysiology and bloom dynamics of Heterosigma akashiwo (Raphidophyceae). In: Anderson DM, Cembella AD, Hallegraeff GM (eds) Physiological ecology of harmful algal blooms. Springer, Berlin, pp 113–131Google Scholar
  31. Terlizzi DE, Ferrier MD, Armbrester EA, Anlauf KA (2002) Inhibition of dinoflagellate growth by extracts of barley straw (Hordeum vulgare). J Appl Phycol 14:275–280CrossRefGoogle Scholar
  32. Yim EC, Park IT, Han HK, Kim SW, Cho H, Kim SJ (2010) Acute toxicity assessment of new algicides of thiazolidinedione derivatives, TD53 and TD49, using Ulva pertusa Kjellman. Korean J Environ Health Toxicol 25:273–278Google Scholar
  33. Yu Z, Sun W, Guo K, Yu S (1992) Allelopathic effects of several aquatic plants on algae. Acta Hydrobiol Sin 16:1–7Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Seung Ho Baek
    • 1
  • Min-Chul Jang
    • 1
  • Moonho Son
    • 1
  • Si Wouk Kim
    • 2
  • Hoon Cho
    • 3
  • Young Ok Kim
    • 1
  1. 1.Korea Institute of Ocean Science and Technology/South Sea InstituteGeojeSouth Korea
  2. 2.Department of Environmental Engineering, Pioneer Research Center for Controlling of Harmful Algal BloomChosun UniversityGwangjuSouth Korea
  3. 3.Department of Polymer Science & EngineeringChosun UniversityGwangjuSouth Korea

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