Journal of Applied Phycology

, Volume 25, Issue 2, pp 399–406 | Cite as

Effect of temperature and light on the photosynthesis as measured by chlorophyll fluorescence of cultured Eucheuma denticulatum and Kappaphycus sp. (Sumba strain) from Indonesia

  • Lideman
  • Gregory N. Nishihara
  • Tadahide Noro
  • Ryuta Terada


The photosynthetic performance of two Indonesian carrageenophytes (Solieriaceae), Eucheuma denticulatum and Kappaphycus sp. (so-called Sumba strain), was investigated under a variety of temperature and light conditions regarding their mariculture performance. A pulse amplitude modulated-chlorophyll fluorometer (Diving-PAM) was used to generate rapid light curves (RLCs) to provide estimates of the relative electron transport rates (rETR) for over 10 temperatures (i.e., from 16 to 34 °C) and at nine levels of photosynthetic active radiation, which ranged from 0 to 1,000 μmol photons m−2 s−1. Underwater irradiance in a cultivation area was also measured at the collection site in South Sulawesi, Indonesia. The initial slope (α), photoinhibition coefficient (β), and the coefficient of maximum photosynthesis assuming no photoinhibition (γ) was calculated by fitting the RLCs to a nonlinear model of the form \( {\text{rETR}} = \gamma \left( {1 - \exp \left( { - \frac{\alpha }{\gamma }{\text{PAR}}} \right)} \right)\left( {\exp \left( { - \frac{\beta }{\gamma }{\text{PAR}}} \right)} \right) \) using a two-level hierarchical Bayesian model. The experiments revealed that E. denticulatum and Kappaphycus sp. required temperatures ranging from 23 to 32 °C and 22 to 33 °C to maintain high rates of photosynthetic activity, respectively. Clearly, both species appear to be well-adapted to the natural light and temperature conditions at the cultivation site, and we expect the results of this study will be useful for the design and sustainable management of similar mariculture activity.


Eucheuma denticulatum Kappaphycus sp. (Sumba strain) Photosynthesis Temperature Irradiance Pulse amplitude modulation (PAM)-chlorophyll fluorometry 



This research was sponsored in part by Grant-in-Aid for Scientific Research (#22510033) from the Japanese Ministry of Education, Culture, Sport, and Technology (RT), and the Nagasaki University Strategy for Fostering Young Scientist with funding provided by the Special Coordination Funds for Promoting Science and Technology of Ministry of Education, Culture, Sport, Science and Technology (GNN). We would like to expresses our thanks to Director of Central for Brackish-water Aquaculture Development Center, for their kind contributions to the collections of samples and measurement of underwater irradiance in their cooperating farming area, South Sulawesi, Indonesia. We also thank Dr. Michael A. Borowitzka, and two reviewers for their valuable suggestion to improve the manuscript. This research was also the part of dissertation submitted by the first author in partial fulfillment of the Ph.D. degree.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Lideman
    • 1
  • Gregory N. Nishihara
    • 2
  • Tadahide Noro
    • 3
  • Ryuta Terada
    • 3
  1. 1.The United Graduate School of Agricultural SciencesKagoshima UniversityKagoshima CityJapan
  2. 2.Institute for East China Sea ResearchNagasaki UniversityNagasaki CityJapan
  3. 3.Faculty of FisheriesKagoshima UniversityKagoshima CityJapan

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