Seasonal variation in phenolic composition, antibacterial and antioxidant activities of Ulva rigida (Chlorophyta) and assessment of antiacetylcholinesterase potential
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The phenolic composition and antibacterial and antioxidant activities of the green alga Ulva rigida collected monthly for 12 months were investigated. Significant differences in antibacterial activity were observed during the year with the highest inhibitory effect in samples collected during spring and summer. The highest free radical scavenging activity and phenolic content were detected in U. rigida extracts collected in late winter (February) and early spring (March). The investigation of the biological properties of U. rigida fractions collected in spring (April) revealed strong antimicrobial and antioxidant activities. Ethyl acetate and n-hexane fractions exhibited substantial acetylcholinesterase inhibitory capacity with EC50 of 6.08 and 7.6 μg mL−1, respectively. The total lipid, protein, ash, and individual fatty acid contents of U. rigida were investigated. The four most abundant fatty acids were palmitic, oleic, linolenic, and eicosenoic acids.
KeywordsUlva rigida Seasonal variation Antimicrobial Antioxidant Acetylcholinesterase inhibition Fatty acid
This work was supported by grants from the Tunisian “Ministry of Higher Education, Scientific Research and Technology”. We wish to thank Pr. Adnane Hammami Head of the Research Laboratory “Micro-organismes et Pathologie Humaine”, EPS Habib Bourguiba, Sfax, Tunisia for providing some of the pathogenic bacterial strains and technical assistance. We thank Mrs Lobna Jlail, technical assistant, for excellent technical GC–MS support in these studies.
- AOAC (2000) Official methods of analysis of AOAC International, 17th edn. Association of Official Analytical Chemists, GaithersburgGoogle Scholar
- Cox S, Abu-Ghannam N, Gupta S (2009) An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int Food Res J 17:205–220Google Scholar
- Dhargalkar VK, Pereira N (2005) Seaweed: promising plant of the millennium. Sci Cult 71:60–66Google Scholar
- Mantena RKR, Wijburg OLC, Vindurampulle C, Bennett-Wood VR, Walduck A, Drummond GR, Davies JK, Robins-Browne RM, Strugnell RA (2008) Reactive oxygen species are the major antibacterials against Salmonella typhimurium purine auxotrophs in the phagosome of RAW 264.7 cells. Cell Microbiol 10:1058–1073PubMedCrossRefGoogle Scholar
- Suganthy N, Karutha Pandian S, Pandima Devi K (2009) Neuroprotective effect of seaweeds inhabiting South Indian coastal area (Hare Island, Gulf of Mannar Marine Biosphere Reserve): cholinesterase inhibitory effect of Hypnea valentiae and Ulva reticulata. Neurosci Lett 468:216–219PubMedCrossRefGoogle Scholar
- Tuney I, Cadirci BH, Unal D, Sukatar A (2006) Antimicrobial activities of the extracts of marine algae from the coast of Urla (Azmir, Turkey). Turk J Biol 30:1–5Google Scholar
- Waterman PG, Mole S (1994) Analysis of phenolic plant metabolites. In: Lawton JH, Likens GE (eds) Methods in ecology. Blackwell, Oxford, pp 36–43Google Scholar