Isolation and analysis of a gene from the marine microalga Isochrysis galbana that encodes a lipase-like protein
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Microalgae constitute a novel study area for characterising new lipolytic enzymes of biotechnological interest. A new gene from the microalga Isochrysis galbana has been isolated and a preliminary characterisation performed. The full-length cDNA contains 2,060 base pairs with an open reading frame of 1,374 nucleotides encoding a polypeptide of 457 amino acids with a predicted molecular mass of 49 kDa, and a theoretical pI of 5.65. The deduced protein includes highly conserved motifs found in α/β fold hydrolases, and shares some similarities with putative or known lipases. Sequence comparison indicated that the catalytic triad corresponds to residues Ser188, Asp306 and His391, with the nucleophilic residue Ser188 positioned within the consensus G-X-S188-X-G pentapeptide. Phylogenetic analyses established close relationships with fungal lipases and microalgal sequences.
KeywordsAlpha/beta hydrolases Isochrysis galbana Lipase Microalgae
We thank Isabelle Martin and Rose-Marie Leroux for their technical assistance. This research received funding from “Laval Agglomération” and the “Conseil Général de la Mayenne”.
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