Journal of Applied Phycology

, Volume 24, Issue 3, pp 467–473 | Cite as

Impacts of glyphosate on photosynthetic behaviors in Kappaphycus alvarezii and Neosiphonia savatieri detected by JIP-test

  • Tong Pang
  • Jianguo Liu
  • Qian Liu
  • Litao Zhang
  • Wei Lin


Neosiphonia savatieri, a filamentous red alga, had spread and caused a massive death of its host Kappaphycus alvarezii since March 2009 in China. With an aim to found a specific method to eliminate the N. savatieri efficiently from carrageenan producing K. alvarezii, the effects of glyphosate on the photosynthetic behaviors of K. alvarezii and N. savatieri were comparatively studied by using fast chlorophyll a (Chl a) fluorescence kinetics. A dose- and time-dependent changes of fast Chl a fluorescence kinetics were obtained in N. savatieri treated by glyphosate, meanwhile no significant change was detected in the K. alvarezii under the same treatment conditions. Moreover, the maximum PSII photochemical efficiency for dark-adapted tissues (F V/F m) of N. savatieri decreased significantly when the N. savatieri was treated with glyphosate. Above results were further supported by transitory offshore glyphosate soak experiment. The brownish-red N. savatieri turned to be olivine then drew off within 5 days after soaking in >1 g L−1 of glyphosate for more than 1 min, meanwhile, no visible harmful effects were detected on K. alvarezii. Based on above results, glyphosate is suggested to be an effective chemical to eliminate N. savatieri from K. alvarezii.


Glyphosate Kappaphycus alvarezii Neosiphonia savatieri Fast chlorophyll a fluorescence 



The authors wish to thank Prof. Michael A. Borowitzka and the reviewers for their insightful and constructive comments. In addition, the authors wish to thank Zhaoliang Zheng from Lingshui Haotian Company for the provision of K. alvarezii. This work was supported by the National Natural Science Foundation of China (30771639) and Special Project for Marine Public Welfare Industry (200705010).


  1. Achnine L, Mata R, Iglesias-Prieto R, Lotina-Hennsen B (1998) Impairment of photosystem II donor side by the natural product odoratol. J Agric Food Chem 46:5313–5317CrossRefGoogle Scholar
  2. Appenroth KJ, Stöckel J, Srivastava A, Strasser RJ (2001) Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements. Environ Pollut 115:49–64PubMedCrossRefGoogle Scholar
  3. Ask E (1999) Cottonii and spinosum cultivation handbook. FMC Food Ingredients Division, Philadelphia, (52)Google Scholar
  4. Ask E, Azanza V (2002) Advances in cultivation of commercial eucheumatoid species: a review with suggestions for future research. Aquaculture 206:257–277CrossRefGoogle Scholar
  5. Baylis A (2000) Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag Sci 56:299–308CrossRefGoogle Scholar
  6. Clark AJ, Landolt W, Bucher JB, Strasser RJ (2000) Beech (Fagus sylvatica) response to ozone exposure assessed with a chlorophyll a fluorescence performance index. Environ Pollut 109:501–507PubMedCrossRefGoogle Scholar
  7. Dai J, Gao H, Dai Y, Zou Q (2004) Changes in activity of energy dissipating mechanisms in wheat flag leaves during senescence. Plant Biol 6:171–177PubMedCrossRefGoogle Scholar
  8. Dawes CJ, Lluisma AO, Trono GC (1994) Laboratory and field growth studies of commercial strains of Eucheuma denticulatum and Kappaphycus alvarezii in the Philippines. J Appl Phycol 6:21–24CrossRefGoogle Scholar
  9. Doty MS, Alvarez VB (1975) Status, problem, advances and economics of Eucheuma farms. Mar Technol Soc J 9:30–35Google Scholar
  10. Duke SO, Baerson SR, Rimando AM (2003) Herbicides: glyphosate. In: Plimmer JR, Gammon DW, Ragsdale NN (eds). Encyclopedia of agrochemicals. Wiley, New York, Available at agr119/ frame.html
  11. Govindjee (1995) Sixty-three years since Kautsky: chlorophyll a fluorescence. Aust J Plant Physiol 22:131–160CrossRefGoogle Scholar
  12. Hurtado AQ, Critchley AT, Trespoey A, Lhonneur GB (2006) Occurrence of Polysiphonia epiphytes in Kappaphycus farms at Calaguas Is., Camarines Norte, Philippines. J Appl Phycol 18:301–306CrossRefGoogle Scholar
  13. Jiang CD, Gao HY, Zou Q (2003) Changes of donor and acceptor side in photosystem 2 complex induced by iron deficiency in attached soybean and maize leaves. Photosynthetica 41:267–271CrossRefGoogle Scholar
  14. King-Díaz B, Barba-Behrens N, Montes-Ayala J, Castillo-Blum SE, Escartín-Guzmán C, Iglesias-Prieto R, Lotina-Hennsen B (1998) Interference by Nickel(II) salts and their 5-methylimidazole-4-carboxylate coordination compounds on the chloroplast redox chain. Z Naturforsh 53c:987–994Google Scholar
  15. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349CrossRefGoogle Scholar
  16. Lazar D (1999) Chlorophyll a fluorescence induction. Biochim Biophys Acta 1412:1–28PubMedCrossRefGoogle Scholar
  17. Liu JG, Pang T, Wang L, Li J, Lin W (2009) The reasons causing catastrophic death in tropical carrageenan producing seaweeds and their difference in resistance to illness. Oceanol Limnol Sin 40:235–241Google Scholar
  18. Lu C, Zhang J (1998) Changes in photosynthesis II function during senescence of wheat leaves. Physiol Plant 104:239–247CrossRefGoogle Scholar
  19. Lu CM, Qiu NW, Wang BS, Zhang JH (2003) Salinity treatment shows no effects on photosystem II photochemistry, but increases the resistance of photosystem II to heat stress in halophyte Suaeda salsa. J Exp Bot 54:851–860PubMedCrossRefGoogle Scholar
  20. Masuda M, Abe T, Kawaguchi S, Phang SM (2001) Taxonomic notes on marine algae from Malaysia VI. Five species of Ceramiales (Rhodophyceae). Bot Mar 44:467–477CrossRefGoogle Scholar
  21. Murchie EH, Chen YZ, Hubbart S, Peng SB, Horton P (1999) Interactions between senescence and leaf orientation determine in situ patterns of photosynthesis and photoinhibition in field-grown rice. Plant Physiol 119:553–563PubMedCrossRefGoogle Scholar
  22. Pang T, Liu J, Liu Q, Lin W (2011) Changes of photosynthetic behaviors in Kappaphycus alvarezii infected by epiphyte. eCAM. doi: 10.1155/2011/658906
  23. Parker HS (1974) The culture of the red algal genus Eucheuma in the Philippines. Aquaculture 3:425–439CrossRefGoogle Scholar
  24. Siehl DL (1997) Inhibitors of EPSPS synthase, glutamine synthetase and histidine synthesis. In: Roe RM, Burton JD, Kuhr RJ (eds) Herbicide activity: toxicology, biochemistry and molecular biology. IOS, Amsterdam, pp 37–67Google Scholar
  25. Srivastava A, Strasser RJ (1997) Constructive and destructive actions of light on the photosynthetic apparatus. J Sci Ind Res 56:133–148Google Scholar
  26. Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol 61:32–42CrossRefGoogle Scholar
  27. Strasser RJ, Tsimilli-Michael M, Srivastava A, Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 321–362Google Scholar
  28. Vairappan CS (2006) Seasonal occurrences of epiphytic algae on the commercially cultivated red alga Kappaphycus alvarezii (Solieriaceae, Gigartinales, Rhodophyta). J Appl Phycol 18:611–617CrossRefGoogle Scholar
  29. Vairappan CS, Chung CS, Hurtado AQ, Soya FE, Bleicher-Lhonneur G, Critchley A (2008) Distribution and symptoms of epiphyte infection in major carrageenophyte-producing farms. J Appl Phycol 20:477–483CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Tong Pang
    • 1
    • 2
  • Jianguo Liu
    • 1
  • Qian Liu
    • 1
  • Litao Zhang
    • 1
  • Wei Lin
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina

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