Abstract
Proteomic profiling on Ecklonia cava Kjellman grown under various seawater temperatures was conducted to search for biomarkers that were useful to evaluate the health of the colonies and formulate actions for the maintenance of marine forests. In the cultivated strains, protein expression was not significantly changed when the cultivation temperature was lowered from 15°C (control) to 10°C. On the contrary, it was markedly changed, i.e., photosynthesis-related proteins were up-regulated and metabolic enzymes were down-regulated, when the temperature was heightened to 20°C. With the cultivation at 30°C, 25 spots within 27 spots expressed at this temperature peculiarly could be identified and classified into ten proteins. Of the distinctive 27 spots at 30°C, 20 spots were detected in the wild strains cultured at the same temperature for a brief time. It is presumed that the proteins including vanadium-dependent bromoperoxidase are heat stress-induced proteins.
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References
Butler A, Carter-Franklin JN (2004) The role of vanadium bromoperoxidase in the biosynthesis of halogenated marine natural products. Nat Prod Rep 21:180–188
Carpentier SC, Witters E, Laukens K, Deckers P, Swennen R, Panis B (2005) Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two-dimensional gel electrophoresis analysis. Proteomics 5:2497–2507
Cosse A, Potin P, Leblanc C (2009) Patterns of gene expression induced by oligoguluronates reveal conserved and environment-specific molecular defence responses in the brown alga Laminaria digitata. New Phytol 182:239–250
Dring MJ (2005) Stress resistance and disease resistance in seaweeds: the role of reactive oxygen metabolism. Adv Bot Res 43:175–207
Edreva A (2005) Generation and scavenging of reactive oxgen species in chloroplast: a submolecular approach. Agric Ecosyst Environ 106:119–133
Görg A, Weiss W, Dunn MJ (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics 4:3665–3685
Hata M, Yokohama Y (1976) Photosynthesis-temperature relationships in seaweeds and their seasonal changes in the colder region of Japan. Bull Jap Soc Phycol 24:1–7 (in Japanese)
Henkel SK, Kawai H, Hofmann GE (2009) Interspecific and interhabitat variation in hsp70 gene expression in native and invasive kelp populations. Mar Ecol Prog Ser 386:1–13
Kawajiri M, Sasaki T, Kageyama Y (1981) Extensive deterioration of the Ecklonia kelp stands and death of the plants, and fluctuations in abundance of the abalone off Toji, southern Izu Peninsula. Bull Shizuoka Pref Fish Stn Stn 15:19–30, in Japanese
Kimura H, Yamauchi S (2009) Current status and problematic points of MOBA restoration. In: Notoya M (ed) Ecology and MOBA restoration of Ecklonia. Kouseisha Kouseikaku, Tokyo, pp 53–71, in Japanese
Latham H (2008) Temperature stress-induced bleaching of the coralline alga Corallina officinalis: a role for the enzyme bromoperoxidase. Biosci Horiz 1:104–113
Lesser MP (1997) Oxidative stress causes coral bleaching during exposure to elevated temperatures. Coral Reefs 16:187–192
Lesser MP (2006) Oxidative stress in marine environments: biochemistry and physiological ecology. Ann Rev Physiol 68:253–278
Maegawa M, Kida W, Aruga Y (1988) A demographic study of the sublittoral brown alga Ecklonia cava Kjellman in coastal water of Shima Peninsula. Japan Jpn J Phycol 36:321–327
McKersie BD, Lesham YY (1994) Stress and stress coping in cultivated plants. Kluwer, Dordrecht, p 260
Miyazawa Y, Yamane S, Guo X, Yamagata T (2005) Ensemble forecast of the Kuroshio meandering. J Geophys Res 110:C10026. doi:10.1029/2004JC002426
Mtolera MSP, Collen J, Pedersen M (1996) Stress-induced production of volatile halogenated organic compounds in Eucheuma denticulatum (Rhodophyta) caused by elevated pH and high light intensities. Eur J Phycol 31:89–95
Nagai K, Yotsukura N, Ikegami H, Kimura H, Morimoto K (2008) Protein extraction for two-dimensional electrophoresis from the lamina of Ecklonia kurome (Laminariales), a recalcitrant tissue containing high levels of viscous polysaccharides. Electrophoresis 29:672–681
Nakayama Y, Arai S (1999) Grazing of the brown alga Ecklonia cava by three herbivorous fishes on the coast of Nakagi, South Izu, central Japan. Jpn J Phycol 47:105–112 (in Japanese)
Pirovani CP, Carvalho HA, Machado RC, Gomes DS, Alvim FC, Pomella AW, Gramacho KP, Cascardo JC, Pereira GA, Micheli F (2008) Protein extraction for proteome analysis from cacao leaves and meristems, organs infected by Moniliophthora perniciosa, the causal agent of the witches’ broom disease. Electrophoresis 29:2391–2401
Ritter A, Ubertini M, Romac S, Gaillard F, Delage L, Mann A, Cock JM, Tonon T, Correa JA, Potin P (2010) Copper stress proteomics highlights local adaption of two strains of the model brown alga Ectocarpus siliculosus. Proteomics 10:2074–2088
Roeder V, Colle’n J, Rousvoal S, Corre E, Leblanc C, Boyen C (2005) Identification of stress gene transcript in Laminaria digitata (Phaeophyceae) protoplast cultures by expressed sequence tag analysis. J Phycol 41:1227–1235
Sakanishi Y, Yokohama Y, Aruga Y (1989) Seasonal changes of photosynthetic activity of a brown alga Ecklonia cava Kjellman. Bot Mag Tokyo 102:37–51
Saravanan RS, Rose JK (2004) A critical evalution of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues. Proteomics 4:2522–2532
Serisawa Y, Yokohama Y, Aruga Y, Tanaka J (2001) Photosynthesis and respiration in bladelets of Ecklonia cava Kjellman (Laminariales, Phaeophyta) in two localities with different temperature conditions. Phycol Res 49:1–11
Taino S (2009) MOBA restoration of Ecklonia cava and E. kurome. In: Notoya M (ed) Ecology and MOBA restoration of Ecklonia. Kouseisha Kouseikaku, Tokyo, pp 72–92 (in Japanese)
Tanaka T, Yamauchi M, Notoya M, Kimura H, Yotsukura N (2007) The morphological diversity and the genetic variation of Ecklonia cava and E. kurome (Laminariales, Phaeophyta) sporophytes collected along the coast of Wakayama prefecture. Aquac Sci 55:1–8 (in Japanese)
Terawaki T, Yamada S, Kawasaki Y (1989) Developmentof techniques for marine macrophyte bed creating in sandy sea bed. II. Growth limiting factors on Eisenia bicyclis and Ecklonia cava in natural sea bed. CRIEPI Research Report U89033, 1–20 (in Japanese)
Tsukidate M, Arai S, Narihara J (1991) Morphological features of Ecklonia cava Kjellman from Kodogawa, northern part of Miyazaki Prefecture, Kyushu. Jpn J Phycol 39:389–391 (in Japanese)
Yokohama Y (1973) A comparative study on photosynthesis-temperature relationships and their seasonal changes in marine benthic algae. Int Rev Ges Hydrobiol 58:463–472
Yotsukura N, Nagai K, Kimura H, Morimoto K (2010) Seasonal changes in proteomic profiles of Japanese kelp: Saccharina japonica (Laminariales, Phaeophyceae). J Appl Phycol 22:443–451
Acknowledgment
The authors thank Dr. H. Ikegami (Kinki University) and Ms. H. Kato (Hokkaido University) for technical assistances. This work was supported partially by grants from Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence of Japan Science and Technology Agency (JST) and the Ministry of Education, Science, Sports and Culture, Japan.
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Yotsukura, N., Nagai, K., Tanaka, T. et al. Temperature stress-induced changes in the proteomic profiles of Ecklonia cava (Laminariales, Phaeophyceae). J Appl Phycol 24, 163–171 (2012). https://doi.org/10.1007/s10811-011-9664-5
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DOI: https://doi.org/10.1007/s10811-011-9664-5