Temperature stress-induced changes in the proteomic profiles of Ecklonia cava (Laminariales, Phaeophyceae)
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Proteomic profiling on Ecklonia cava Kjellman grown under various seawater temperatures was conducted to search for biomarkers that were useful to evaluate the health of the colonies and formulate actions for the maintenance of marine forests. In the cultivated strains, protein expression was not significantly changed when the cultivation temperature was lowered from 15°C (control) to 10°C. On the contrary, it was markedly changed, i.e., photosynthesis-related proteins were up-regulated and metabolic enzymes were down-regulated, when the temperature was heightened to 20°C. With the cultivation at 30°C, 25 spots within 27 spots expressed at this temperature peculiarly could be identified and classified into ten proteins. Of the distinctive 27 spots at 30°C, 20 spots were detected in the wild strains cultured at the same temperature for a brief time. It is presumed that the proteins including vanadium-dependent bromoperoxidase are heat stress-induced proteins.
Keywords2-DE Kelp Protein profiles Ecklonia cava Phaeophyta Temperature stress
The authors thank Dr. H. Ikegami (Kinki University) and Ms. H. Kato (Hokkaido University) for technical assistances. This work was supported partially by grants from Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence of Japan Science and Technology Agency (JST) and the Ministry of Education, Science, Sports and Culture, Japan.
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