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Journal of Applied Phycology

, Volume 22, Issue 3, pp 305–311 | Cite as

Role of secondary metabolites as defense chemicals against ice-ice disease bacteria in biofouler at carrageenophyte farms

  • Charles S. Vairappan
  • Sangeetha P. Anangdan
  • Kai Lee Tan
  • Shigeki Matsunaga
Article

Abstract

Carrageenophyte farming is an expanding economical activity in North Borneo Island, Malaysia. During routine monitoring of “ice-ice” disease and epiphyte outbreak at commercial farms, it was apparent that culture lines were heavily (60–80%) infested with biofoulers, particularly Acanthophora spp. and Laurencia majuscula. However, only L. majuscula showed dominance and flourished even during “ice-ice” disease outbreak. Presence of chemical defense against seaweed pathogens was investigated in two populations of L. majuscula collected from three major carrageenophyte farms in two districts; (A) Lohok Butun, Selakan Island, and Bum-Bum Island, in Semporna district, and (B) Telutuh, Carrington Reef, and Balambangan Island, in Kudat district. The first population contained elatol (1), and iso-obtusol (2), and, second population contained (Z)-10,15-dibromo-9-hydroxy-chamigra-1,3(15),7(14)-triene (3) and (E)-10-15-dibromo-9-hydroxy-chamigra-1,3(15),7(14)-triene (4), as their antibacterial metabolites. All four metabolites showed highly selective inhibition against “ice-ice” disease bacteria compared to human pathogens at 30 µg disk−1. In addition, seasonal variation of these compounds at two representative farms (Selakan Island [P-1] and Balambangan Island [P-2]) revealed a 120–170% increase in concentration during “ice-ice” disease outbreak. Microscopy of fresh specimens showed the presence of corps en cerise, which is the synthesis and storage site of halogenated metabolites at superficial cortical cells, branch tips, and trichoblasts. This suggests the importance of these metabolites as defense chemicals against “ice-ice” disease bacteria in L. majuscula that grows on seaweed culture lines.

Keywords

Carrageenophyte Biofouler Ice-ice disease Laurencia majuscula Chamigrenes Defense chemicals 

Notes

Acknowledgments

We are grateful to Sabah Parks for the support and assistance during field survey. This study was funded by the “International Foundation for Science (IFS)” and Committee for the Prohibition of Chemical Weapon, Hague, The Netherlands (IFS) and Japanese Society for the Promotion of Science (P 06215).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Charles S. Vairappan
    • 1
  • Sangeetha P. Anangdan
    • 1
  • Kai Lee Tan
    • 1
  • Shigeki Matsunaga
    • 2
  1. 1.Laboratory of Natural Products Chemistry, Institute for Tropical Biology and ConservationUniversiti Malaysia SabahKota KinabaluMalaysia
  2. 2.Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agriculture and Life ScienceThe University of TokyoTokyoJapan

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