Abstract
The effects of light intensity and temperature on Arthrospira platensis growth and production of extracellular polymeric substances (EPS) in batch culture were evaluated using a three-level, full-factorial design and response surface methodology. Three levels were tested for each parameter (temperature: 30, 35, 40°C; light intensity: 50, 115, 180 μmol photons m−2 s−1). Both growth and EPS production are influenced mainly by the temperature factor but the interaction term temperature*light intensity also had a significant effect. In addition, conditions optimising EPS production are different from those optimising growth. The highest growth rate (0.414 ± 0.003 day−1) was found at the lowest temperature (30°C) and highest light intensity (180 μmol photons m−2 s−1) tested, no optima were detectable within the given test range. Obviously, optima for growth must be at a temperature lower than 30°C and a light intensity higher than 180 μmol photons m−2 s−1. For EPS production, light intensity had a positive linear effect (optimum obviously higher than 180 μmol photons m−2 s−1), but for the temperature parameter a maximum effect was detectable at 35°C.
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Trabelsi, L., Ben Ouada, H., Bacha, H. et al. Combined effect of temperature and light intensity on growth and extracellular polymeric substance production by the cyanobacterium Arthrospira platensis . J Appl Phycol 21, 405–412 (2009). https://doi.org/10.1007/s10811-008-9383-8
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DOI: https://doi.org/10.1007/s10811-008-9383-8