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Early development of germlings of Sargassum thunbergii (Fucales, Phaeophyta) under laboratory conditions

  • Ziguo Zhao
  • Fengjuan Zhao
  • Jianting Yao
  • Jingmei Lu
  • Put. O. AngJr.
  • Delin Duan
Article

Abstract

Morphology and culture studies on germlings of Sargassum thunbergii (Mertens et Roth) Kuntze were carried out under controlled laboratory conditions. Growth characteristics of these germlings grown under different temperatures (from 10 to 25°C), irradiances (from 9 to 88 μmol photons m−2 s−1), and under blue and white light conditions are described. The development of embryonic germlings follows the classic “8 nuclei 1 egg” type described for Sargassaceae. Fertilized eggs spent 5–6 h developing into multicellular germlings with abundant rhizoids after fertilization. Under conditions of 20°C, 44 μmol photons m−2 s−1 and photoperiod of 12 h, young germlings with one or two leaflets reached 2–3 mm in length after 8 weeks. Temperature variations (10, 15, 20, 25°C) under 88 μmol photons m−2 s−1 significantly influenced the growth rate within the first week, although this effect became less obvious after 8 weeks, especially at 15 and 20°C. Variation in germling growth was highly significant under different irradiances (9, 18, 44, 88 μmol photons m−2 s−1) at 25°C. Low temperature (10°C) reduced germling growth. Growth of germlings cultured under blue light was lower than in white light. Optimal growth of these germlings occurred at 25°C and 44 μmol photons m−2 s−1.

Keywords

Sargassum thunbergii Germling Growth and development Temperature Irradiance 

Notes

Acknowledgements

The authors gratefully acknowledge NSFC (40618001), N_CUHK438/06 and the Shandong Agriculture Seedstock Breeding Project for financial assistance, and the anonymous reviewers for their constructive comments. Thanks are also kindly given to Mr. J.D. Liu for his assistance in the field sampling.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ziguo Zhao
    • 1
    • 2
  • Fengjuan Zhao
    • 2
  • Jianting Yao
    • 2
  • Jingmei Lu
    • 1
  • Put. O. AngJr.
    • 3
  • Delin Duan
    • 2
  1. 1.School of Life SciencesNortheast Normal UniversityChangchunChina
  2. 2.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  3. 3.The Chinese University of Hong KongShantin, N. T.China

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