Journal of Analytical Chemistry

, Volume 60, Issue 7, pp 636–643 | Cite as

Optimized and Validated Spectrophotometric Methods for the Determination of Roxatidine Acetate Hydrochloride in Drug Formulations Using 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone and p-Chloranilic Acid

  • Rahman Nafisur
  • Kashif Mohammad


Two simple and sensitive spectrophotometric procedures are proposed for the determination of roxatidine in pure form and in tablets. Both methods are based on the charge transfer complexation reaction of the drug with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) or p-chloranilic acid resulting in colored products. In both cases, the absorbance measurement was made at 530 nm. Beer’s law was obeyed in the concentration ranges 5–65 and 10–260 µg mL−1 with DDQ and p-chloranilic acid, respectively. A series of variables were studied to optimize the reaction conditions. No interference was observed from the common excipients present in the formulations. The described methods were applied successfully for the analysis of roxatidine in pharmaceutical preparations. Statistical comparison of the results with those from our UV reference method shows excellent agreement. Both methods are validated statistically and do differ significantly in accuracy.


Benzoquinone Reference Method Spectrophotometric Method Complexation Reaction Pure Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Martindale: The Extra Pharmacopoeia, 31st ed, Reynolds, J.E.F., Ed., London: Pharmaceutical, 1996.Google Scholar
  2. 2.
    Collin, J.D. and Pidgen, A.W., Drugs, 1988, vol. 35, p. 41.Google Scholar
  3. 3.
    Lameire, N., et al., Drugs, 1988, vol. 35, p. 48.Google Scholar
  4. 4.
    Ficcara, R., Ficcara, P., Tommasini, S., Calabro, M.L., Acta Technol. Legis. Med., 1993, vol. 2, p. 109.Google Scholar
  5. 5.
    Anant, P.A. and Shankar, S.K., Indian Drugs, 1995, vol. 7, p. 333.Google Scholar
  6. 6.
    Dimov, N. and Ivanov, C., J. Chromatogr., 1992, vol. 2, p. 368.CrossRefGoogle Scholar
  7. 7.
    Shibata, K., Itaya, T., Yamakoshi, N., Kurata, S., and Zumi, N., Iyakuhin Kenkyu, 1985, vol. 6, p. 485.Google Scholar
  8. 8.
    Veronico, M., Ragno, G., Carlucci, G., and Vetuschi, C., Inst. J. Pharm., 1995, vol. 1, p. 109.CrossRefGoogle Scholar
  9. 9.
    Askal, H.F., Talanta, 1997, vol. 44, p. 1749.CrossRefGoogle Scholar
  10. 10.
    Bebaway, L.I., Elkousy, N.M., Suddik, J.K., and Shokry, M., J. Pharm. Biomed. Anal., 1999, vol. 21, p. 133.CrossRefPubMedGoogle Scholar
  11. 11.
    Khashaba, P.Y., Shabouri, S.R., Emara, K.M., and Mohammad, A.M., J. Pharm. Biomed. Anal., 2000, vol. 22, p. 363.CrossRefPubMedGoogle Scholar
  12. 12.
    Elsayed, M.A. and Agarwal, S.P., Talanta, 1982, vol. 29, p. 535.CrossRefGoogle Scholar
  13. 13.
    Adikwu, M.U., Bull. Chem. Farm., 1996, vol. 135, p. 15.Google Scholar
  14. 14.
    Rahman, N. and Azmi, S.N.H., Anal. Sci., 2000, vol. 16, p. 1353.CrossRefGoogle Scholar
  15. 15.
    Rahman, N. and Hoda, M.N., J. Pharm. Biomed. Anal., 2003, vol. 31, p. 381.CrossRefPubMedGoogle Scholar
  16. 16.
    Foster, R., Organic Charge Transfer Complexes, London: Academic, 1969.Google Scholar
  17. 17.
    Slifkin, M.A., Smith, B.M., and Walmsley, R.H., Spectrochim. Acta, 1969, vol. 25, p. 1479.CrossRefGoogle Scholar
  18. 18.
    Douglas, A.S. and Donald, M.W., Principles of Instrumental Analysis, New York: Holt, Rinehart, and Winston, 1971.Google Scholar
  19. 19.
    Benesi, H.A. and Heldebrand, J.H., J. Am. Chem. Soc., 1949, vol. 71, p. 2703.CrossRefGoogle Scholar
  20. 20.
    Ross, S.R. and Labes, M.L., J. Am. Chem. Soc., 1957, vol. 79, p. 76.CrossRefGoogle Scholar
  21. 21.
    Morelli, B., Analyst, 1983, vol. 108, p. 870.CrossRefGoogle Scholar
  22. 22.
    Morelli, B., J. Pharm. Biomed. Anal., 1987, vol. 5, p. 577.CrossRefGoogle Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • Rahman Nafisur
    • 1
  • Kashif Mohammad
    • 1
  1. 1.Analytical Research Division, Department of ChemistryAligarh Muslim University, Aligarh (U.P.)India

Personalised recommendations