Journal of Analytical Chemistry

, Volume 60, Issue 5, pp 480–485 | Cite as

Study on the Determination of Heavy-Metal Ions in Tobacco and Tobacco Additives by Microwave Digestion and HPLC with PAD Detection

  • G. Yang
  • Z. Li
  • H. Shi
  • J. Wang


A new method for the simultaneous determination of heavy-metal ions in tobacco and tobacco additive by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The tobacco and tobacco additive samples were digested by microwave digestion. The lead, cadmium, mercury, nickel, copper, and tin ions in the digested samples were precolumn derivated with tetra-(4-aminophenyl)-porphyrin (T4-APP) to form color chelates; the Hg-T4-APP, Cd-T4-APP, Pb-T4-APP, Ni-T4-APP, Cu-T4-APP, and Sn-T4-APP chelates were then enriched by solid-phase extraction with C18 disks and the retained chelates were eluted from the disks using tetrahydrofuran (THF). The chelates were separated on a Waters Xterra™ RP18 column by gradient using methanol (containing 0.05 mol/L pyrrolidine-acetic acid buffer salt, pH 10.0) and acetone (containing 0.05 mol/L pyrrolidine-acetic acid buffer salt, pH 10.0) as a mobile phase at a flow rate of 0.5 mL/min and detected with a photodiode array detector in the range 350–600 nm. The detection limits of lead, cadmium, mercury, nickel, copper, and tin were 5, 4, 2.5, 5, 8, and 4 ng/L, respectively, in the original samples. The method was applied to the determination of lead, cadmium, mercury, nickel, copper, and tin in tobacco and tobacco additive with good results.


Nickel Mercury Cadmium Porphyrin Tetrahydrofuran 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Ohashi, H., Uehara, N., and Shijo, Y., J. Chromatogr., 1991, vol. 539, p. 225.CrossRefGoogle Scholar
  2. 2.
    Ryan, E. and Meaney, M., Analyst, 1992, vol. 117, p. 1435.CrossRefGoogle Scholar
  3. 3.
    Uehara, N., Jinno, K.I., Hashimoto, M., and Shijo, Y., J.Chromatogr., A, 1997, vol. 789, p. 395.Google Scholar
  4. 4.
    Wang, H., Zhang, H.S., and Cheng, J.K., Talanta, 1999, vol. 48, p. 1.CrossRefGoogle Scholar
  5. 5.
    Vachirapatma, N., Doble, P., and Haddad, P.R., Anal. Chim. Acta, 2000, vol. 409, p. 35.CrossRefGoogle Scholar
  6. 6.
    Inhinoki, S., Okutani, T., Konakawa, N., Nakano, T., and Fujii, Y., J. Chromatogr. Sci., 2001, vol. 39, p. 405.PubMedGoogle Scholar
  7. 7.
    Zhang, X.S., Shi, L., Zhang, L.F., You, C.S., and Lin, C.S., J. Chromatogr., A, 1997, vol. 789, p. 485.Google Scholar
  8. 8.
    Miura, J.I., Anal. Chem., 1990, vol. 62, p. 1424.CrossRefGoogle Scholar
  9. 9.
    Ichinoki, S., Hongo, N., and Yamazaki, M., Anal. Chem., 1988, vol. 60, p. 2099.CrossRefGoogle Scholar
  10. 10.
    Palmieri, M.D. and Fritz, J.S., Anal. Chem., 1988, vol. 60, p. 2244.CrossRefGoogle Scholar
  11. 11.
    Main, M.V. and Fritz, J.S., Talanta, 1991, vol. 38, p. 253.CrossRefGoogle Scholar
  12. 12.
    Wu, X.L., Shun, D., and Yue, H.Y., Chinese, Fenxi Shiyansi, 1998, vol. 17, no.4, p. 70.Google Scholar
  13. 13.
    Zhao, Y., Chromatografia, 2000, vol. 51, p. 231.Google Scholar
  14. 14.
    Dilli, S. and Tong, P., Anal. Chim. Acta, 1999, vol. 395, p. 101.CrossRefGoogle Scholar
  15. 15.
    Ali, A., Yin, X.F., and Shen, H., Anal. Chim. Acta, 1998, vol. 369, p. 215.CrossRefGoogle Scholar
  16. 16.
    Raynaud, F.I., Mistry, P., Donaghue, A., and Poon, G.K., Cancer Chemother. Phamacol., 1996, vol. 38, p. 155.CrossRefGoogle Scholar
  17. 17.
    Wang, S.F. and Wai, C.M., J. Chromatogr. Sci., 1994, vol. 32, p. 504.Google Scholar
  18. 18.
    Valle, A.I., Gonzalez, M.J., and Marina, M.L., J. Chromatogr., 1992, vol. 607, p. 207.CrossRefGoogle Scholar
  19. 19.
    Marina, M.L., Andres, P., and Dfez-Masa, J.C., Chromatographia, 1993, vol. 35, p. 621.Google Scholar
  20. 20.
    Shi, Z.H. and Fu, C.G., Fenxi Ceshi Jishu Yu Yiqi, 1996, vol. 2, no.3, p. 28.Google Scholar
  21. 21.
    Shi, Z.H. and Fu, C.G., Talanta, 1997, vol. 44, p. 593.CrossRefGoogle Scholar
  22. 22.
    Biesaga, M., Pyrzynska, K., and Tropjanowicz, M., Talanta, 2000, vol. 51, p. 509.CrossRefGoogle Scholar
  23. 23.
    Robards, K., Start, P., and Patsalides, E., Analyst, 1991, vol. 116, p. 1247.CrossRefGoogle Scholar
  24. 24.
    Cheng, J.K. and Zhang, X.X., Fenxi Huaxue, 1990, vol. 18, p. 876.Google Scholar
  25. 25.
    Cheng, J.K., Gaodeng Xuexiao Huaxue Xuebao, 1995, vol. 16, p. 696.Google Scholar
  26. 26.
    Wang, P. and Lee, H.K., J. Chromatogr., A, 1997, vol. 789, p. 437.Google Scholar
  27. 27.
    Timerbaev, A.R., Petrukhin, O.M., and Zolotov, Y.A., Fresenius’ Z. Anal. Chem., 1987, vol. 327, p. 87.CrossRefGoogle Scholar
  28. 28.
    Wang, Y.E. and Zhou, T.Z., Fenxi Shiyansi, 1999, vol. 18, no.5, p. 91.Google Scholar
  29. 29.
    Zhaiig, X.S. and Lin, C.S., Fenxi Huaxue, 1994, vol. 22, p. 847.Google Scholar
  30. 30.
    Stahlberg, J., J. Chromatogr., A, 1999, vol. 855, p. 3.Google Scholar
  31. 31.
    Malik, A.K., Seidel, B.S.., and Faubel, W., Chem. Environ. Res., 2000, vol. 9, p. 3.Google Scholar
  32. 32.
    Liu, W.Y. and Huang, J.H., Liang Xin-Miao Sepu, 1986, vol. 4, p. 206.Google Scholar
  33. 33.
    Xu, X.J., Zhang, H.S., Zhang, C.Y., and Chang, J.K., Anal. Chem., 1991, vol. 63, p. 2532.CrossRefPubMedGoogle Scholar
  34. 34.
    Yan, D.R., Zhang, J., and Schwedt, G., Gaodeng Xuexiao Huaxue Xuebao, 1990, vol. 11, p. 136.Google Scholar
  35. 35.
    Yin, J.W., Wang, G.J., and Xiao, Z.F., Liang Xin-Miao Sepu, 2000, vol. 18, p. 436.Google Scholar
  36. 36.
    Chen, X.H., Zhu, Y., and Yang, J.J., Fenxi Huaxue, 2000, vol. 28, p. 1260.Google Scholar
  37. 37.
    Igarashi, S., Ide, N., and Takagai, Y., Anal. Chem. Acta, 2000, vol. 424, p. 263.CrossRefGoogle Scholar
  38. 38.
    Kobayashi, M., Saitoh, K., and Suzuki, N., Chromatographia, 1985, vol. 20, no.2, p. 72.Google Scholar
  39. 39.
    Saitoh, K., Shabata, Y., and Suzuki, R., J. Chromatogr., 1991, vol. 542, p. 351.CrossRefGoogle Scholar
  40. 40.
    Yuan, Y.X., Fenxi Huaxue, 1990, vol. 18, p. 87.Google Scholar
  41. 41.
    Xu, X.I., Zhang, H.S., Zhang, C.Y., and Cheng, I.K., Gaodeng Huexiao Huaxue Xuebao, 1990, vol. 11, p. 945.Google Scholar
  42. 42.
    Shi, Z.H., Wang, Y.H., and Fu, C.G., Liang Xin-Miao Sepu, 2000, vol. 18, p. 27.Google Scholar
  43. 43.
    Li, H.C. and Wang, S.Y., Analytical Methods of Tobacco Additives, Zhangzhou, China: Henan Science and Technology Press, 1998, p. 324.Google Scholar
  44. 44.
    Yang, G.Y., Li, Z., Wang, L., and Wang, B.X., Chin. J. Tobacco Sci. Res., 2001, vol. 2, no.1, p. 53.Google Scholar
  45. 45.
    Lei, Y.W., Ge, C.C., and Zeng, D.Z., Huaxue Shiji, 1994, vol. 16, no.2, p. 105.Google Scholar
  46. 46.
    Lei, Y.W., Ge, C.C., and Zeng, D.Z., Huaxue Shiji, 1994, vol. 16, no.2, p. 105.Google Scholar
  47. 47.
    Fang, M. and Hu, S.K., Yekuang Ceshi, 1991, vol. 10, p. 318.Google Scholar
  48. 48.
    Zeng Yune, Zhang Huashan, and Chen Zhenhua, Handbook of Modern Chemical Reagents, the Forth Fascicule. Chromogenic Agent of Inorganic Ion, Beijing: Chemical Industry Press, 1989.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • G. Yang
    • 1
    • 2
  • Z. Li
    • 1
  • H. Shi
    • 1
  • J. Wang
    • 2
  1. 1.Department of ChemistryYunnan UniversityKunmingChina
  2. 2.Key Laboratory of Chemistry and EngineeringYunnan Academy of Tobacco ScienceKunmingChina

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