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l-Lactic acid biosensor based on multi-layered graphene

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Abstract

Pristine graphene platelets and graphene oxide were used as electrode modifiers, aiming the investigation of their electrochemical efficacy towards β-nicotinamide adenine dinucleotide (NADH). The electrochemical detection of NADH is one of the most studied areas of bioelectroanalysis because of the ubiquity of NAD(P)H-based enzymatic reactions in nature. Commercially available graphene and laboratory prepared graphene oxide were used to modify glassy carbon electrodes and the behaviour of such modified electrodes against potassium ferricyanide (III) and NADH was reported. Relying on the graphene-modified transducer, l-lactic dehydrogenase (l-LDH) was successfully immobilised in a 1 % Nafion® membrane. The developed biosensor, working at +250 mV versus Ag/AgCl reference electrode, was used to assess l-lactic acid in four different types of yogurts, revealing an l-lactic acid concentration ranging between 0.3 and 0.6 %.

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Abbreviations

NADH:

β-nicotinamide adenine dinucleotide

NAD+ :

Oxidised form of β-nicotinamide adenine dinucleotide

G:

Graphene

GO:

Graphene oxide

GC:

Modified glassy carbon

l-LDH:

l-lactate dehydrogenase

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Acknowledgments

This work was financially supported by the Romanian Ministry of Education, Research and Innovation thorough PN-II-RU-TE-2009-1 national program, under the project identification code TE_44.

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Authors and Affiliations

  1. National Institute for Research and Development in Microtechnology (IMT-Bucharest), 126A Erou Iancu Nicolae, 077190, Bucharest, Romania

    Antonio Radoi, Alexandru Cosmin Obreja, Adina Bragaru & Adrian Dinescu

  2. Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031, Bucharest, Romania

    Sandra A. V. Eremia & Gabriel-Lucian Radu

Authors
  1. Antonio Radoi
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  2. Alexandru Cosmin Obreja
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  3. Sandra A. V. Eremia
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  4. Adina Bragaru
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  6. Gabriel-Lucian Radu
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Correspondence to Antonio Radoi.

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Radoi, A., Obreja, A.C., Eremia, S.A.V. et al. l-Lactic acid biosensor based on multi-layered graphene. J Appl Electrochem 43, 985–994 (2013). https://doi.org/10.1007/s10800-013-0594-6

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