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Using electrophoretic deposition to identify protein charge in biological medium

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Abstract

Protein adsorption is the first step involved in establishing a suitable integration between a biomaterial and host tissue. It is therefore of highest interest to know the electric charge of proteins present in the relevant medium to be able to predict the behaviour of cells on given surfaces. In this study, electrophoretic deposition (EPD) was used as a simple method to identify the charge of proteins present in biological medium. In the model experiment carried out here, EPD was conducted using a biological medium containing 10% fetal calf serum (FCS) and the charge of the protein was determined by examining the migration of the protein in the EPD cell under a certain applied voltage. In addition, the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.

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Acknowledgment

The authors thank Dr. M. G. Ardakani (Imperial College London) for his support with SEM observations.

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Authors and Affiliations

  1. Department of Materials, Imperial College London, Prince Consort Road, London, SW7 2AZ, UK

    Decheng Meng & Aldo R. Boccaccini

  2. Department of Molecular and Applied Biosciences, University of Westminster, London, W1W 6UW, UK

    Lydia Francis & Ipsita Roy

  3. Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany

    Aldo R. Boccaccini

Authors
  1. Decheng Meng
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  2. Lydia Francis
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  3. Ipsita Roy
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  4. Aldo R. Boccaccini
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Corresponding author

Correspondence to Aldo R. Boccaccini.

Additional information

Decheng Meng and Lydia Francis share first co-authorship status.

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Meng, D., Francis, L., Roy, I. et al. Using electrophoretic deposition to identify protein charge in biological medium. J Appl Electrochem 41, 919–923 (2011). https://doi.org/10.1007/s10800-011-0317-9

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  • DOI: https://doi.org/10.1007/s10800-011-0317-9

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