Journal of Applied Electrochemistry

, Volume 40, Issue 9, pp 1651–1657 | Cite as

Direct electrochemistry of myoglobin immobilized in NiO/MWNTs hybrid nanocomposite for electrocatalytic detection of hydrogen peroxide

  • Jian-Ding Qiu
  • San-Guan Cui
  • Min-Qiang Deng
  • Ru-Ping Liang
Original Paper


A novel matrix, multiwalled carbon nanotubes supported nickel oxide nanoparticles composite nanomaterial (NiO@MWNTs), for immobilization of protein and biosensing was designed using a simple and effective hydrothermal method. Using myoglobin (Mb) as a model, the direct electrochemistry of immobilized Mb indicated the matrix could accelerate the electron transfer between protein’s active sites and the electrode. The modified electrode shows excellent electrocatalytic activity toward the reduction of H2O2 without the help of an electron mediator. The simple operation, fast response, acceptable stability, and reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.


Hydrothermal NiO nanoparticle Multiwalled carbon nanotubes Myoglobin Direct electrochemistry 



We greatly appreciate the supports of the National Natural Science Foundation of China (20605010; 20865003; 20805023), the Jiangxi Province Natural Science Foundation (0620039; 2007JZH2644) and the Opening Foundation of State Key Laboratory of Chem/Biosensing and Chemometrics of Hunan University (2006022; 2007012).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jian-Ding Qiu
    • 1
    • 2
  • San-Guan Cui
    • 1
  • Min-Qiang Deng
    • 1
  • Ru-Ping Liang
    • 1
  1. 1.Department of Chemistry and Institute for Advanced StudyNanchang UniversityNanchangPeople’s Republic of China
  2. 2.Department of Chemical EngineeringPingxiang CollegePingxiangPeople’s Republic of China

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