Abstract
The effect of irradiation by power ultrasound on the adsorption of proteins on copper has been investigated, using bovine serum albumin (BSA) as a model protein in pH 7 phosphate buffer solution. Open circuit potential measurements, cyclic voltammetry and electrochemical impedance spectroscopy were used to characterise the copper/solution interface. Electrochemical impedance measurements at potentials close to the open circuit potential showed that pulsed ultrasound irradiation removes the naturally formed copper oxide films in phosphate buffer solution, and that their re-formation can lead to an oxide film with different electrical characteristics. Adsorption of BSA blocks the surface, decreasing or increasing the interfacial resistance, depending on the applied potential and the oxide characteristics, as well as changing the interfacial capacitance. This study augurs well for application of the combination of electrochemical impedance plus ultrasound to other systems.
Similar content being viewed by others
References
Bourdon E, Loreau N, Blache D (1999) FASEB J 13:233
Emerson TE Jr (1989) Crit Care Med 17:690
Stamler JS, Singel DJ, Loscalzo J (1992) Science 258:1898
Liu M, Zhang Y, Yang M, Deng C, Xie Q, Yao S (2006) Polymer 47:3372
Xie Q, Xiang C, Yuan Y, Zhang Y, Nie L, Yao S (2003) J Colloid Interface Sci 262:107
Menz B, Knerr R, Göpferich A, Steinem C (2005) Biomaterials 26:237
Anzai J, Guo B, Osa T (1996) Bioelectrochem Bioenerg 40:35
Cheng XL, Roscoe SG (2005) Biomaterials 26:7350
Frateur I, Lartundo-Rojas L, Methivier C, Galtayries A, Marcus P (2006) Electrochim Acta 51:1550
Juliette LY, Hyman MR, Arp DJ (1995) J Bacteriol 177:4908
Cai Y, Xie Q, Zhou A, Zhang Y, Yao SJ (2001) Biochem Biophys Methods 47:209
Hepel M, Stobiecka M (2007) Bioelectrochemistry 70:155
Matysik FM, Matysik S, Oliveira Brett AM, Brett CMA (1997) Anal Chem 69:1651
Oliveira Brett AM, Silva LA, Brett CMA (2002) Langmuir 18:2326
Pound BG, Gorfu Y, Schattner P, Mortelmans KE (2005) Corrosion 61:452
Morais NLPA, Brett CMA (2002) J Appl Electrochem 32:653
Ashassi-Sorkhabi H, Ghalebsaz-Jeddi N (2006) Ultrason Sonochem 13:180
Touyeras F, Hihn JY, Bourgoin X, Jacques B, Hallez L, Branger V (2005) Ultrason Sonochem 12:13
Zerbino JO, Gassa LM (2003) J Solid State Electrochem 7:177
Protopopoff E, Marcus P (2005) Electrochim Acta 51:408
Zerbino JO, de Mele MFL (1997) J Appl Electrochem 27:335
Anfinsen CB, Edsall JT, Richards FM (1985) Advances in protein chemistry, vol 37. Academic Press, New York
Lewis JA (2000) J Am Ceram Soc 83:2341
Acknowledgements
The authors acknowledge financial support from EU-Alfa project PETRA II (ALFA_II-0343-FA-FCD-FI), from Fundação para a Ciência e Tecnologia (FCT), Portugal for a PhD grant (SFRH/BD/31483/2006) for E.M.P., from ICEMS/FCT, Research Unit 103, Coimbra-Portugal and Instituto de Física Gleb Wataghin, Campinas-Brazil.
Author information
Authors and Affiliations
Corresponding author
Additional information
Special issue dedicated to Prof. Tony Wragg.
Rights and permissions
About this article
Cite this article
Pinto, E.M., Soares, D.M. & Brett, C.M.A. Influence of ultrasound irradiation on the adsorption of bovine serum albumin on copper. J Appl Electrochem 37, 1367–1373 (2007). https://doi.org/10.1007/s10800-007-9382-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10800-007-9382-5