Journal of Applied Electrochemistry

, Volume 37, Issue 11, pp 1367–1373 | Cite as

Influence of ultrasound irradiation on the adsorption of bovine serum albumin on copper

  • Edilson M. Pinto
  • David M. Soares
  • Christopher M. A. Brett
Original Paper


The effect of irradiation by power ultrasound on the adsorption of proteins on copper has been investigated, using bovine serum albumin (BSA) as a model protein in pH 7 phosphate buffer solution. Open circuit potential measurements, cyclic voltammetry and electrochemical impedance spectroscopy were used to characterise the copper/solution interface. Electrochemical impedance measurements at potentials close to the open circuit potential showed that pulsed ultrasound irradiation removes the naturally formed copper oxide films in phosphate buffer solution, and that their re-formation can lead to an oxide film with different electrical characteristics. Adsorption of BSA blocks the surface, decreasing or increasing the interfacial resistance, depending on the applied potential and the oxide characteristics, as well as changing the interfacial capacitance. This study augurs well for application of the combination of electrochemical impedance plus ultrasound to other systems.


Ultrasound irradiation Power ultrasound Copper Bovine serum albumin Electrochemical impedance spectroscopy 



The authors acknowledge financial support from EU-Alfa project PETRA II (ALFA_II-0343-FA-FCD-FI), from Fundação para a Ciência e Tecnologia (FCT), Portugal for a PhD grant (SFRH/BD/31483/2006) for E.M.P., from ICEMS/FCT, Research Unit 103, Coimbra-Portugal and Instituto de Física Gleb Wataghin, Campinas-Brazil.


  1. 1.
    Bourdon E, Loreau N, Blache D (1999) FASEB J 13:233Google Scholar
  2. 2.
    Emerson TE Jr (1989) Crit Care Med 17:690CrossRefGoogle Scholar
  3. 3.
    Stamler JS, Singel DJ, Loscalzo J (1992) Science 258:1898CrossRefGoogle Scholar
  4. 4.
    Liu M, Zhang Y, Yang M, Deng C, Xie Q, Yao S (2006) Polymer 47:3372CrossRefGoogle Scholar
  5. 5.
    Xie Q, Xiang C, Yuan Y, Zhang Y, Nie L, Yao S (2003) J Colloid Interface Sci 262:107CrossRefGoogle Scholar
  6. 6.
    Menz B, Knerr R, Göpferich A, Steinem C (2005) Biomaterials 26:237CrossRefGoogle Scholar
  7. 7.
    Anzai J, Guo B, Osa T (1996) Bioelectrochem Bioenerg 40:35CrossRefGoogle Scholar
  8. 8.
    Cheng XL, Roscoe SG (2005) Biomaterials 26:7350CrossRefGoogle Scholar
  9. 9.
    Frateur I, Lartundo-Rojas L, Methivier C, Galtayries A, Marcus P (2006) Electrochim Acta 51:1550CrossRefGoogle Scholar
  10. 10.
    Juliette LY, Hyman MR, Arp DJ (1995) J Bacteriol 177:4908Google Scholar
  11. 11.
    Cai Y, Xie Q, Zhou A, Zhang Y, Yao SJ (2001) Biochem Biophys Methods 47:209CrossRefGoogle Scholar
  12. 12.
    Hepel M, Stobiecka M (2007) Bioelectrochemistry 70:155CrossRefGoogle Scholar
  13. 13.
    Matysik FM, Matysik S, Oliveira Brett AM, Brett CMA (1997) Anal Chem 69:1651CrossRefGoogle Scholar
  14. 14.
    Oliveira Brett AM, Silva LA, Brett CMA (2002) Langmuir 18:2326CrossRefGoogle Scholar
  15. 15.
    Pound BG, Gorfu Y, Schattner P, Mortelmans KE (2005) Corrosion 61:452CrossRefGoogle Scholar
  16. 16.
    Morais NLPA, Brett CMA (2002) J Appl Electrochem 32:653CrossRefGoogle Scholar
  17. 17.
    Ashassi-Sorkhabi H, Ghalebsaz-Jeddi N (2006) Ultrason Sonochem 13:180CrossRefGoogle Scholar
  18. 18.
    Touyeras F, Hihn JY, Bourgoin X, Jacques B, Hallez L, Branger V (2005) Ultrason Sonochem 12:13CrossRefGoogle Scholar
  19. 19.
    Zerbino JO, Gassa LM (2003) J Solid State Electrochem 7:177Google Scholar
  20. 20.
    Protopopoff E, Marcus P (2005) Electrochim Acta 51:408CrossRefGoogle Scholar
  21. 21.
    Zerbino JO, de Mele MFL (1997) J Appl Electrochem 27:335CrossRefGoogle Scholar
  22. 22.
    Anfinsen CB, Edsall JT, Richards FM (1985) Advances in protein chemistry, vol 37. Academic Press, New YorkGoogle Scholar
  23. 23.
    Lewis JA (2000) J Am Ceram Soc 83:2341CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Edilson M. Pinto
    • 1
    • 2
  • David M. Soares
    • 2
  • Christopher M. A. Brett
    • 1
  1. 1.Departamento de QuímicaUniversidade de CoimbraCoimbraPortugal
  2. 2.Instituto de Física Gleb WataghinUnicampCampinasBrazil

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