Abstract
Potential-controlled chromatography is introduced as a new technique for the separation of amino acids and peptides. The principle of potential-controlled chromatography depends on the use of electrically conductive material as the stationary phase of the chromatographic column. Thus from an electrochemical point of view the packed column can be regarded as a packed-bed electrode. The electrical potential of this stationary phase can be controlled by a potentiostat. The separation of amino acid and peptide molecules during their migration through the column depends on their own electric charge on the one hand and on the electrical potential of the stationary phase on the other. The chromatographic separation of some amino acids could be demonstrated.
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Acknowledgement
This research (No. 9968N) was supported by Arbeitsgemeinschaft industrieller Forschungsvereinigungen e.V. through the German Federal Ministry of Economics and Labour.
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Kocak, F., Vuorilehto, K., Schrader, J. et al. Potential-controlled chromatography for the separation of amino acids and peptides. J Appl Electrochem 35, 1231–1237 (2005). https://doi.org/10.1007/s10800-005-9034-6
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DOI: https://doi.org/10.1007/s10800-005-9034-6