Ocular biometry through fully refocused steady-state magnetic resonance imaging sequence: reliability and agreement with the IOLMaster® 500



To evaluate the reliability and agreement between Fully Refocused Steady-State magnetic resonance sequences (FRSS) and the IOLMaster® 500 optical biometer for measuring anterior chamber depth (ACD) and axial length (AL).


In a sample of 32 healthy volunteers, separate observers measured the ACD and AL of both eyes using both techniques (inter-method) and through repeated FRSS measurements (interobserver) and by the same observer (intraobserver). We employed the Bland–Altman method to determine the agreement between FRSS and partial coherence interferometry (using the IOLMaster®) and the interobserver and intraobserver variability, providing the limits of agreement (LoA, or mean difference ± 1.96 SD). Correlation coefficients and intraclass correlation coefficients were also provided.


For ACD measurements with FRSS in pseudo-color scale, we obtained an LoA of 0.016 ± 0.266 mm compared with partial coherence interferometry. For AL with FRSS in greyscale, the LoA was 0.019 ± 0.383 mm. Maximum interobserver variability showed a  − 0.036 ± 0.247 mm LoA for ACD with FRSS in pseudo-color scale. Maximum intraobserver variability was 0.000 ± 0.157 mm LoA for AL with FRSS in greyscale.


ACD and AL measurements using FRSS sequencing present high LoA and reliability when compared with partial coherence interferometry using the IOLMaster® 500. The results were better for FRSS in pseudo-color scale in ACD determination and for FRSS in greyscale in AL determination. FRSS would not be recommended for IOL power calculation due to variability of AL measurement.

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This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Author information




LIPS, JGV and JJGH contributed to the study conception and design. Data collection was performed by LIPS, JGV, JJGH and FFM. Material preparation and analysis were performed by all authors. The first draft of the manuscript was written by LIPS, JGV and MSL. All authors commented on early versions of the manuscript, and all authors read and approved the final manuscript.

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Correspondence to Lorenzo Ismael Perez-Sanchez.

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Perez-Sanchez, L.I., Gutierrez-Vazquez, J., Satrustegui-Lapetra, M. et al. Ocular biometry through fully refocused steady-state magnetic resonance imaging sequence: reliability and agreement with the IOLMaster® 500. Int Ophthalmol (2021). https://doi.org/10.1007/s10792-021-01748-7

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  • Magnetic resonance imaging
  • Interferometry
  • Eye axial length
  • Anterior chamber