To investigate whether plasma high sensitivity C-reactive protein (hs-CRP) level is associated with exudative age-related macular degeneration (AMD) as well as variants of ARMS2 A69S and CFH I62V in patients with exudative AMD.
A case-control study was done comparing CRP among patients with exudative AMD including those with polypoidal choroidal vasculopathy, typical AMD and retinal angiomatous proliferation, and CRP were also compared between cases and controls. Plasma CRP was measured from peripheral blood using latex nepherometry for all participants. Genotyping of ARMS2 A69S and CFH I62V was performed for all patients with exudative AMD using TaqMan technology.
Among 125 patients with exudative AMD, including 31 with typical neovascular AMD, 73 with PCV and 21 with RAP lesions and 150 controls, CRP levels were higher in exudative AMD than in controls. (P = 2.7 × 10–5) There was not a significant difference in hs-CRP levels among AMD subtypes. Neither variants of ARMS2 nor CFH was associated with hs-CRP level in patients with exudative AMD. A multiple regression analysis revealed that gender male, presence of exudative AMD and presence of cardiovascular diseases were associated with increased plasma hs-CRP.
Plasma hs-CRP was elevated independent of variants of ARMS2 A69S and CFH I62V in patients with exudative AMD.
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Kawasaki R, Yasuda M, Song SJ, Chen SJ, Jonas JB, Wang JJ, Mitchell P, Wong TY (2010) The prevalence of age-related macular degeneration in Asians: a systematic review and meta-analysis. Ophthalmology 117:921–927
Sakurada Y, Yoneyama S, Sugiyama A, Tanabe N, Kikushima W, Mabuchi F, Kume A, Kubota T, Iijima H (2016) Prevalence and genetic characteristics of geographic atrophy among elderly japanese with age-related macular degeneration. PLoS ONE 11:e0149978
Pepys MB, Hirschfield GM (2003) C-reactive protein: a critical update. J Clin Invest 111:1805–1812
Tutuncu Y, Satman I, Celik S, Dinccag N, Karsidag K, Telci A, Genc S, Issever H, Tuomilehto J, Omer B (2016) A comparison of hs-CRP levels in new diabetes groups diagnosed based on FPG, 2-hPG, or HbA1c criteria. J Diabetes Res 2016:5827041
Chen JY, Zhu XL, Liu WH, Xie Y, Zhang HF, Wang X, Ying R, Chen ZT, Wu MX, Qiu Q, Wang JF, Chen YX (2020) C-reactive protein derived from perivascular adipose tissue accelerates injury-induced neointimal hyperplasia. J Transl Med 18:68
Seddon JM, Gensler G, Milton RC, Klein ML, Rifai N (2004) Association between C-reactive protein and age-related macular degeneration. JAMA 291:704–710
Seddon JM, Gensler G, Rosner B (2010) C-reactive protein and CFH, ARMS2/HTRA1 gene variants are independently associated with risk of macular degeneration. Ophthalmology 117:1560–1566
Fernandez-Robredo P, Recalde S, Hernandez M, Zarranz-Ventura J, Molins B, Casaroli-Marano RP, Adan A, Saenz-de-Viteri M, Garcia-Layana A (2018) Novel association of high C-reactive protein levels and A69S at risk alleles in wet age-related macular degeneration women. Front Immunol 9:1862
Barrea L, Arnone A, Annunziata G, Muscogiuri G, Laudisio D, Salzano C, Pugliese G, Colao A, Savastano S (2019) Adherence to the mediterranean diet, dietary patterns and body composition in women with polycystic ovary syndrome (PCOS). Nutrients 11(10):2278
Sugiyama A, Sakurada Y, Honda S, Miki A, Matsumiya W, Yoneyama S, Kikushima W, Iijima H (2018) Retreatment of exudative age-related macular degeneration after loading 3-monthly intravitreal ranibizumab. Ophthalmologica 239:52–59
Sakurada Y, Nakamura Y, Yoneyama S, Mabuchi F, Gotoh T, Tateno Y, Sugiyama A, Kubota T, Iijima H (2015) Aqueous humor cytokine levels in patients with polypoidal choroidal vasculopathy and neovascular age-related macular degeneration. Ophthalmic Res 53:2–7
Kikuchi M, Nakamura M, Ishikawa K, Suzuki T, Nishihara H, Yamakoshi T, Nishio K, Taki K, Niwa T, Hamajima N, Terasaki H (2007) Elevated C-reactive protein levels in patients with polypoidal choroidal vasculopathy and patients with neovascular age-related macular degeneration. Ophthalmology 114:1722–1727
Wang JJ, Ross RJ, Tuo J, Burlutsky G, Tan AG, Chan CC, Favaloro EJ, Williams A, Mitchell P (2008) The LOC387715 polymorphism, inflammatory markers, smoking, and age-related macular degeneration. A population-based case-control study. Ophthalmology 115:693–699
Ambreen F, Ismail M, Qureshi IZ (2015) Association of gene polymorphism with serum levels of inflammatory and angiogenic factors in Pakistani patients with age-related macular degeneration. Mol Vis 21:985–999
Wang J, Xue Y, Thapa S, Wang L, Tang J, Ji K (2016) Relation between age-related macular degeneration and cardiovascular events and mortality: a systematic review and meta-analysis. Biomed Res Int 2016:8212063
Sakurada Y, Kubota T, Mabuchi F, Imasawa M, Tanabe N, Iijima H (2008) Association of LOC387715 A69S with vitreous hemorrhage in polypoidal choroidal vasculopathy. Am J Ophthalmol 145:1058–1062
Sakurada Y, Kubota T, Imasawa M, Tsumura T, Mabuchi F, Tanabe N, Iijima H (2009) Angiographic lesion size associated with LOC387715 A69S genotype in subfoveal polypoidal choroidal vasculopathy. Retina 29:1522–1526
Sakurada Y, Kubota T, Imasawa M, Mabuchi F, Tateno Y, Tanabe N, Iijima H (2011) Role of complement factor H I62V and age-related maculopathy susceptibility 2 A69S variants in the clinical expression of polypoidal choroidal vasculopathy. Ophthalmology 118:1402–1407
Yoneyama S, Sakurada Y, Kikushima W, Sugiyama A, Tanabe N, Mabuchi F, Kubota T, Iijima H (2016) Genetic factors associated with choroidal vascular hyperpermeability and subfoveal choroidal thickness in polypoidal choroidal vasculopathy. Retina 36:1535–1541
Yasuma TR, Nakamura M, Nishiguchi KM, Kikuchi M, Kaneko H, Niwa T, Hamajima N, Terasaki H (2010) Elevated C-reactive protein levels and ARMS2/HTRA1 gene variants in subjects without age-related macular degeneration. Mol Vis 16:2923–2930
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Shijo, T., Sakurada, Y., Fukuda, Y. et al. Association of CRP levels with ARMS2 and CFH variants in age-related macular degeneration. Int Ophthalmol (2020). https://doi.org/10.1007/s10792-020-01460-y
- C-reactive protein
- Exudative age-related macular degeneration
- ARMS2 A69S
- CFH I62V