Abstract
The aim of the study is to describe the experience with precut facility for endothelial keratoplasty at a single eye bank affiliated to a tertiary eye care center in India. Data on precut tissues from Nov 2012 to Dec 2014 were retrospectively reviewed from the electronic database of the eye bank of a tertiary eye care center in South India. Donor characteristic data including donor age, precut and postcut endothelial cell density (ECD), recipient age, and thickness of graft were collected. The number of precuts increased from 42 in 2012 (Nov and Dec) and 422 in 2013 to 584 in 2014. Of the total of 1048 precuts, seven (0.67 %) were miscut and could not be utilized for transplants. The donor age ranged from 2 to 89 years. A mean change of 43.6 ± 325.2 cells/mm2 in ECD was noted after cut, proportional increase in mean being 1.9 %. The change in ECD after cut was negatively correlated with ECD before cut. The recipient age ranged from 1 to 89 years. The median thickness of donor lenticule after cut was 148 µm (interquartile range 131–166 µm). Analysis of precut donor corneas from a single eye bank shows that the ECD of the processed tissues was excellent for transplantation. The tissue wastage in the hands of eye bank personal was minimal.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chen ES, Terry MA, Shamie N, Hoar KL, Friend DJ (2008) Precut tissue in Descemet’s stripping automated endothelial keratoplasty donor characteristics and early postoperative complications. Ophthalmology 115(3):497–502
Nelson BA, Ritenour RJ (2014) Tissue quality of eye-bank-prepared precut corneas for Descemet’s stripping automated endothelial keratoplasty. Can J Ophthalmol 49(1):92–95
Katzman LR, Hoover CK, Khalifa YM, Jeng BH (2015) Assessment of the accuracy and cut-failure rates of eye bank-cut corneas for use in endothelial keratoplasty: a comparison of outcomes between 2010 and 2013. Cornea 34(11):1365–1368
Price MO, Baig KM, Brubaker JW, Price FW Jr (2008) Randomized, prospective comparison of precut versus surgeon-dissected grafts for descemet stripping automated endothelial keratoplasty. Am J Ophthalmol 146(1):36–41
Liu YC, Alvarez Paraz CM, Cajucom-Uy HY, Agahari D, Sethuraman S, Tan DT et al (2014) Risk factors for donor endothelial loss in eye bank-prepared posterior lamellar corneal tissue for descemet stripping automated endothelial keratoplasty. Cornea 33(7):677–682
Kelliher C, Engler C, Speck C, Ward D, Farazdaghi S, Jun AS (2009) A comprehensive analysis of eye bank-prepared posterior lamellar corneal tissue for use in endothelial keratoplasty. Cornea 28(9):966–970
Price MO, Price FW Jr, Stoeger C, Soper M, Locke GD, Bavuso T (2008) Central thickness variation in precut DSAEK donor grafts. J Cataract Refract Surg 34(9):1423–1424
Acknowledgments
The authors acknowledge the support of Mr Hariharan K, Associate Director, Ramayamma International Eye Bank (RIEB), Hyderabad, India, and the team members of RIEB.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Research Committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.
Rights and permissions
About this article
Cite this article
Mohamed, A., Chaurasia, S., Chandragiri, V. et al. Precut cornea for Descemet’s stripping endothelial keratoplasty: experience at a single eye bank. Int Ophthalmol 37, 525–529 (2017). https://doi.org/10.1007/s10792-016-0297-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10792-016-0297-1