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Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model

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Abstract

To compare the effects of different concentrations of topical human amniotic fluid (HAF) in a mouse model of dry eye, forty C57BL/6 mice were divided into 4 treatment groups: 20 % HAF, 50 % HAF, 100 % HAF, and isotonic salt solution (control). Dry eye was induced by an injection of botulinum toxin B into the lacrimal gland. Tear production, ocular surface fluorescein staining, and blink rate were evaluated in each mouse at 5 time points during a 4-week period. Goblet cell density was assessed in stained histological sections. Regarding tear production, 20, 50, and 100 % HAF groups were all different from the control group (P < 0.001) at week 1. However, there were no statistically significant differences between the 20, 50, and 100 % HAF groups. At week 2, 20, 50, and 100 % HAF groups had significant improvement in staining score and were significantly different from the control group (P = 0.047, P = 0.005, and P = 0.001, respectively). No difference in spontaneous blink rate was observed between groups, at any time point. Goblet cell density was significantly decreased in the control group compared to the HAF treatment groups. All tested concentrations of topical HAF were effective and superior than the control in this keratoconjunctivitis sicca-induced mouse model. Further studies are needed to evaluate the effects of HAF on the human ocular surface.

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Acknowledgments

This study was supported by Research to Prevent Blindness Inc., New York, NY.

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Authors and Affiliations

  1. Department of Ophthalmology, The Wilmer Ophthalmological Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Guilherme G. Quinto, Juan Castro-Combs, Li Li, Navin Gupta & Ashley Behrens

  2. Department of Ophthalmology, Vision Institute, Federal University of São Paulo, São Paulo, Brazil

    Guilherme G. Quinto & Mauro Campos

  3. The Wilmer Ophthalmological Institute, 400 N. Broadway Street, Smith Building Suite 455, Baltimore, MD, 21231, USA

    Ashley Behrens

Authors
  1. Guilherme G. Quinto
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  2. Juan Castro-Combs
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  3. Li Li
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  4. Navin Gupta
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  5. Mauro Campos
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  6. Ashley Behrens
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Correspondence to Ashley Behrens.

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Quinto, G.G., Castro-Combs, J., Li, L. et al. Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model. Int Ophthalmol 36, 643–650 (2016). https://doi.org/10.1007/s10792-016-0180-0

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  • DOI: https://doi.org/10.1007/s10792-016-0180-0

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