Curcumin–galactomannoside complex inhibits pathogenesis in Ox-LDL-challenged human peripheral blood mononuclear cells
- 13 Downloads
Oxidised low-density lipoprotein (ox-LDL) is a pro-atherogenic molecule, which induces inflammatory response and contributes to the pathogenesis of vascular dysfunction to atherosclerosis. The aim of the present study was to explore the anti-inflammatory effect of a novel bioavailable formulation of curcumin as ‘curcumagalactomannosides’ (CGM) against ox-LDL-induced inflammatory responses in human peripheral blood mononuclear cells (hPBMCs). Curcumagalactomannosides was made from natural curcumin using the soluble dietary fibre (galactomannans) derived from fenugreek seeds (Trigonella foenumgracum) and the hPBMCs were isolated from healthy human volunteers. The cells were cultured in collagen-coated plates at 37 °C and grouped as Group I (Control), Group II (ox-LDL treated) and Group III (ox-LDL + CGM treated). Further analysis of inflammatory markers, reactive oxygen species and mRNA expression levels indicated significantly increased expressions of iNOS, TNF-α, IL-6 and VCAM-1 in ox-LDL-treated group along with the nuclear translocation of NF-κB. Other inflammatory markers such as LOX, PGE2, total COX and lipid peroxidation level were also found to be significantly (p < 0.05) increased upon ox-LDL treatment. The treatment with CGM on the other hand was found to down-regulate and reverse the ox-LDL-induced alterations indicating its potential anti-inflammatory effect on hPBMCs via. NF-κB signalling pathway.
KeywordsAtherosclerosis Cytokines Curcumagalactomannosides Curcumin Inflammation Oxidised LDL
This work was supported by M/s Akay Flavours & Aromatics Pvt Ltd, Cochin, India.
Compliance with ethical standards
Conflict of interest
Authors disclose the conflict of interest. CGM is the patented curcumin formulation of M/s Akay Flavours & Aromatics Pvt Ltd, Cochin, India, trademarked as CurQfen®.
- Axelrod B, Cheesebrough TM, Laakso B (1981) Lipoxygenase from soybeans methods in enzymology, vol 71. Academic Press, Cambridge, pp 441–453Google Scholar
- Chen Q, Fisher DT, Clancy KA, Gauguet JM, Wang WC, Unger E, Rose-John S, von Andrian UH, Baumann H, Evans SS (2006) Fever-range thermal stress promotes lymphocyte trafficking across high endothelial venules via an interleukin 6 trans-signaling mechanism. Nat Immunol 7:1299–1308CrossRefPubMedGoogle Scholar
- Cominacini L, Garbin U, FrattaPasini A, Paulon T, Davoli A, Campagnola M, Marchi E, Pastorino AM, Gaviraghi G, Lo Cascio V (1997) Antioxidants inhibit the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 induced by oxidized ldl on human umbilical vein endothelial cells. Free Radic Biol Med 22:117–127CrossRefPubMedGoogle Scholar
- Luoma JS, Stralin P, Marklund SL, Hiltunen TP, Sarkioja T, Yla-Herttuala S (1988) Expression of extracellular SOD and iNOS in macrophages and smooth muscle cells in human and rabbit atherosclerotic lesions: colocalization with epitopes characteristic of oxidized LDL and peroxynitrite-modified proteins. Arterioscler Thromb Vasc Biol 18:157–167CrossRefGoogle Scholar
- Ratheesh M, Svenia JP, Asha S, Sandhya S, Grishkumar B, Krishnakumar IM (2017) Anti-inflammatory effect of a novel formulation of coconut inflorescence sap against ox-LDL induced inflammatory responses in human peripheral blood mononuclear cells by modulating TLR-NF-κB signaling pathway. Toxicol Mech Methods 27:615–621CrossRefPubMedGoogle Scholar