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Orofacial antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis in rodents

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Abstract

This study aimed to evaluate the antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis (PLS) using rodent models of orofacial pain. Acute pain was induced by formalin, capsaicin, cinnamaldehyde, acidified saline or glutamate (cutaneous modes) and hypertonic saline (corneal model). In one experiment, animals were pretreated with ruthenium red, glibenclamide, naloxone, L-NAME, methylene blue or ketamine to investigate the mechanism of antinociception. In another experiment, animals pretreated with PLS or saline were submitted to the temporomandibular joint formalin test. In yet another, animals were submitted to craniofacial pain induced by mustard oil. Motor activity was evaluated with the open-field test. Cytotoxicity and antioxidant activities were also assessed. Pre-treatment with PLS significantly reduced nociceptive behavior associated with acute pain. Antinociception was effectively reduced, but not inhibited, by ruthenium red and ketamine. L-NAME and glibenclamide enhanced the PLS effect. PLS antinociception was resistant to methylene blue, naloxone and heating. PLS presented no cytotoxicity or antioxidant properties. Our results confirm the potential pharmacological relevance of PLS as an inhibitor of orofacial nociception in acute pain probably mediated by glutamatergic, nitrergic, TRPs and K + ATP pathways.

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Acknowledgements

The authors would like to thank CNPq, Funcap and Capes for financial support and Fundação Edson Queiroz for infrastructure.

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Authors and Affiliations

  1. Experimental Biology Center (NUBEX), University of Fortaleza (UNIFOR), Av. Washington Soares, 1321, Edson Queiroz, Fortaleza, Ceará, CEP 60811-905, Brazil

    Caio Átila Prata Bezerra Souza, Breytiner Amaro de Oliveira, Sacha Aubrey Alves Rodrigues Santos, José de Maria Albuquerque de Melo Júnior, Francisco Rogenio da Silva Mendes, Lana Karine Vasconcelos Barroso, Francisco Ernani Alves Magalhães, Angelo Roncalli Alves e Silva & Adriana Rolim Campos

  2. Laboratório de Bioprospecção de Produtos Naturais e Biotecnologia, Universidade Estadual do Ceará (UECE), Tauá, Fortaleza, Ceará, Brazil

    Francisco Lucas Alves Batista & Francisco Ernani Alves Magalhães

  3. Departamento de Engenharia de Pesca, Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil

    Francisco Regivânio Nascimento Andrade, Eugênio Januário Ramos Neto & Wladimir Ronald Lobo Farias

  4. Embrapa Agroindústria Tropical, Fortaleza, Ceará, Brazil

    Kirley Marques Canuto

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  1. Caio Átila Prata Bezerra Souza
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Correspondence to Adriana Rolim Campos.

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Souza, C.Á.P.B., de Oliveira, B.A., Santos, S.A.A.R. et al. Orofacial antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis in rodents. Inflammopharmacol 27, 261–269 (2019). https://doi.org/10.1007/s10787-018-0454-4

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