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Poly-ADP-ribose polymerase inhibition provides protection against lung injury in a rat paraquat toxicity model

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Abstract

Objectives

Paraquat (PQ) is a widely used herbicide. Exposure to PQ at toxic doses can result in fatal acute lung injury. Inhibition of the poly-(ADP-ribose) polymerase (PARP) enzyme alleviates inflammation and necrosis in various pathologies. Here we aimed to evaluate the effects of PARP inhibition on PQ-induced lung damage in a rat experimental model.

Methods

Female Sprague-Dawley rats (n = 24) were allocated into three groups: sham, PQ and PQ + 3-aminobenzamide (3-AB) that is a PARP inhibitor, groups. Experimental lung injury was induced by administration of 15 mg/kg PQ intraperitoneally in PQ and PQ + 3-AB groups. 3-AB (10 mg/kg twice per day) was administered to the PQ + 3-AB group for four consecutive days. The animals were killed on the fifth day following PQ administration. Lung tissue and blood samples were collected and stored until analysis.

Results

Serum lactate dehydrogenase (LDH) and neopterin levels, tissue oxidative stress parameters, transforming growth factor-beta1 (TGF-β) levels and histological injury scores in the PQ + 3-AB group were significantly lower than in the PQ group (P < 0.05, PQ vs. PQ + 3-AB). Total antioxidant capacity in the PQ + 3-AB group was significantly higher than in the PQ group (P < 0.05, PQ + 3-AB vs. PQ).

Conclusion

Our results suggested that the use of PARP inhibitors following PQ toxicity might be useful for minimizing lung injury due to paraquat toxicity.

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Authors and Affiliations

  1. Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey

    Salim Kemal Tuncer

  2. Department of Anesthesia and Reanimation, Dr. Abdurrahman Yurtaslan, Ankara Oncology Education and Research Hospital, Ankara, Turkey

    Seher Altinel

  3. Department of Forensic Medicine, Gulhane Military Medical Academy, Ankara, Turkey

    Mehmet Toygar

  4. Department of Public Health, Gulhane Military Medical Academy, Ankara, Turkey

    Hakan Istanbulluoglu & Recai Ogur

  5. Department of Biophysics, Gulhane Military Medical Academy, Ankara, Turkey

    Kahraman Ates

  6. Department of Noncommissioned Officer Health College, Gulhane Military Medical Academy, Ankara, Turkey

    Ozcan Altinel

  7. Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey

    Yildirim Karslioglu

  8. Department of Physiology, Gulhane Military Medical Academy, Ankara, 06010, Turkey

    Turgut Topal, Ahmet Korkmaz & Bulent Uysal

Authors
  1. Salim Kemal Tuncer
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  2. Seher Altinel
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  3. Mehmet Toygar
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  4. Hakan Istanbulluoglu
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  6. Recai Ogur
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  7. Ozcan Altinel
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  8. Yildirim Karslioglu
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  9. Turgut Topal
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  10. Ahmet Korkmaz
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  11. Bulent Uysal
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Corresponding author

Correspondence to Bulent Uysal.

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Tuncer, S.K., Altinel, S., Toygar, M. et al. Poly-ADP-ribose polymerase inhibition provides protection against lung injury in a rat paraquat toxicity model. Inflammopharmacol 24, 155–161 (2016). https://doi.org/10.1007/s10787-016-0268-1

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  • DOI: https://doi.org/10.1007/s10787-016-0268-1

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