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Inflammopharmacology

, Volume 24, Issue 4, pp 155–161 | Cite as

Poly-ADP-ribose polymerase inhibition provides protection against lung injury in a rat paraquat toxicity model

  • Salim Kemal Tuncer
  • Seher Altinel
  • Mehmet Toygar
  • Hakan Istanbulluoglu
  • Kahraman Ates
  • Recai Ogur
  • Ozcan Altinel
  • Yildirim Karslioglu
  • Turgut Topal
  • Ahmet Korkmaz
  • Bulent Uysal
Original Article

Abstract

Objectives

Paraquat (PQ) is a widely used herbicide. Exposure to PQ at toxic doses can result in fatal acute lung injury. Inhibition of the poly-(ADP-ribose) polymerase (PARP) enzyme alleviates inflammation and necrosis in various pathologies. Here we aimed to evaluate the effects of PARP inhibition on PQ-induced lung damage in a rat experimental model.

Methods

Female Sprague-Dawley rats (n = 24) were allocated into three groups: sham, PQ and PQ + 3-aminobenzamide (3-AB) that is a PARP inhibitor, groups. Experimental lung injury was induced by administration of 15 mg/kg PQ intraperitoneally in PQ and PQ + 3-AB groups. 3-AB (10 mg/kg twice per day) was administered to the PQ + 3-AB group for four consecutive days. The animals were killed on the fifth day following PQ administration. Lung tissue and blood samples were collected and stored until analysis.

Results

Serum lactate dehydrogenase (LDH) and neopterin levels, tissue oxidative stress parameters, transforming growth factor-beta1 (TGF-β) levels and histological injury scores in the PQ + 3-AB group were significantly lower than in the PQ group (P < 0.05, PQ vs. PQ + 3-AB). Total antioxidant capacity in the PQ + 3-AB group was significantly higher than in the PQ group (P < 0.05, PQ + 3-AB vs. PQ).

Conclusion

Our results suggested that the use of PARP inhibitors following PQ toxicity might be useful for minimizing lung injury due to paraquat toxicity.

Keywords

Paraquat toxicity Lung injury PARP inhibition Neopterin TGF-β1 

Notes

Compliance with ethical standards

Conflict of interest

The authors report no declarations of interest. We have not been funded by any individual or organization.

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Copyright information

© Springer International Publishing 2016

Authors and Affiliations

  • Salim Kemal Tuncer
    • 1
  • Seher Altinel
    • 2
  • Mehmet Toygar
    • 3
  • Hakan Istanbulluoglu
    • 4
  • Kahraman Ates
    • 5
  • Recai Ogur
    • 4
  • Ozcan Altinel
    • 6
  • Yildirim Karslioglu
    • 7
  • Turgut Topal
    • 8
  • Ahmet Korkmaz
    • 8
  • Bulent Uysal
    • 8
  1. 1.Department of Emergency MedicineGulhane Military Medical AcademyAnkaraTurkey
  2. 2.Department of Anesthesia and ReanimationDr. Abdurrahman Yurtaslan, Ankara Oncology Education and Research HospitalAnkaraTurkey
  3. 3.Department of Forensic MedicineGulhane Military Medical AcademyAnkaraTurkey
  4. 4.Department of Public HealthGulhane Military Medical AcademyAnkaraTurkey
  5. 5.Department of BiophysicsGulhane Military Medical AcademyAnkaraTurkey
  6. 6.Department of Noncommissioned Officer Health CollegeGulhane Military Medical AcademyAnkaraTurkey
  7. 7.Department of PathologyGulhane Military Medical AcademyAnkaraTurkey
  8. 8.Department of PhysiologyGulhane Military Medical AcademyAnkaraTurkey

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