Antihypernociceptive activity of anethole in experimental inflammatory pain
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Anethole has been reported to have antioxidant, antibacterial, antifungal, antiinflammatory, and anesthetic properties. In the present study, we evaluated the effects of anethole in two pain models of inflammatory origin: acute inflammation induced by carrageenan and persistent inflammation induced by Complete Freund’s adjuvant. We evaluated the effects of anethole (125, 250, and 500 mg/kg) on the development of paw oedema and mechanical hypernociception. The liver was collected for histological analysis. Paw skin was collected to determine the levels of the cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-17 (IL-17), and myeloperoxidase activity. Blood was collected to assess alanine transaminase (ALT) and aspartate transaminase (AST). The chemical composition of star anise oil was determined by gas chromatography/mass spectrometry (GC/MS), showing a presence of anethole of 98.1 %. Oral pretreatment with anethole in mice inhibited paw oedema, mechanical pernociception, myelopewroxidase activity, TNF-α, IL-1β and IL-17 levels in acute and persistent inflammation models. Additionally, anethole treatment did not alter prostaglandin E2-induced mechanical hypernociception. Possible side effects were also examined. Seven-day anethole treatment did not alter plasma AST and ALT levels, and the histological profile of liver tissue was normal. The present study provides evidence of the antiinflammatory and analgesic activities of anethole in acute and persistent inflammation models.
KeywordsIllicium verum Anethole Oedema Hypernociception Anti-nociceptive activity
We gratefully acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Araucária (FA) for financial support.
- Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectroscopy, 4th edn. Allured Publishing, Carol StreamGoogle Scholar
- Chen Q, Muramoto K, Masaaki N, Ding Y, Yang H, Mackey M, Li W, Inoue Y, Ackermann K, Shirota H, Matsumoto I, Spyvee M, Schiller S, Sumida T, Gusovsky F, Lamphier M (2010) A novel antagonist of the prostaglandin E2 EP4 receptor inhibits Th1 differentiation and Th17 expansion and is orally active in arthritis models. Br J Pharmacol 160:292–310PubMedCrossRefGoogle Scholar
- Cunha TM, Barsante MM, Guerrero AT, Verri WA Jr, Ferreira SH, Coelho FM, Bertini R, Di Giacinto C, Allegretti M, Cunha FQ, Teixeira MM (2008) Treatment with DF 2162, a non-competitive allosteric inhibitor of CXCR1/2, diminishes neutrophil influx and inflammatory hypernociception in mice. Br J Pharmacol 154:460–470PubMedCrossRefGoogle Scholar
- Domiciano TP, Bersani-Amado CA (2011) 22f. Efeito do anetol sobre a resposta inflamatória aguda e crônica. Dissertação de Mestrado (Programa de Pós-Graduação em Ciências Farmacêutica), Universidade Estadual de Maringá, ParanáGoogle Scholar
- Nguelefack TB, Dutra RC, Paszcuk AF, Andrade EL, Tapondjou LA, Calixto JB (2010) Antinociceptive activities of the methanol extract of the bulbs of Dioscorea bulbifera L. var sativa in mice is dependet of No-cGMT-ATP-sensitive-K+ channel activation. J Ethnopharmacol 128:567–574PubMedCrossRefGoogle Scholar
- Queiroz AC, de Lira DP, Dias TLMF, de Souza ET, da Matta CBB, de Aquino AB, Silva LHAC, da Silva DJC, Mella EAC, Agra MF, Filho JM, de Araujo-Junior JX, Santos BVO, Moreira MSA (2010) The antinociceptive and anti-inflammatory activities of Piptadenia stipulacea Benth (Fabaceae). J Ethnopharmacol 128:377–383PubMedCrossRefGoogle Scholar
- Stolina M, Bolon B, Middleton S, Dwyer D, Brown H, Duryea D, Zhu L, Rohner A, Pretorius J, Kostenuik P, Feige U, Zack D (2009) The evolving systemic and local biomarker milieu at different stages of disease progression in rat adjuvant-induced arthritis. J Clin Immunol 29:158–174PubMedCrossRefGoogle Scholar
- Teixeira JM (2006) Fisiopatologia da dor aguda e crônica. In: Teixeira MJ, Dor (eds) Manual para clínico. Atheneu, São Paulo, pp 9–23Google Scholar
- Valério DA, Cunha TM, Arakawa NS, Lemos HP, Da Costa FB, Parada CA, Ferreira SH, Cunha FQ, Verri WA Jr (2007) Anti-inflammatory and analgesic effects of the sesquiterpene lactone budlein A in mice: inhibition of cytokine production-dependent mechanism. Eur J Pharmacol 562:155–163PubMedCrossRefGoogle Scholar
- Verri WA Jr, Cunha TM, Magro DA, Guerrero AT, Vieira SM, Carregaro V, Souza GR, Henriques MG, Ferreira SH, Cunha FQ (2009) Targeting endothelin ETA and ETB receptors inhibits antigen-induced neutrophil migration and mechanical hypernociception in mice. Naunyn Schmiedebergs Arch Pharmacol 379:271–279PubMedCrossRefGoogle Scholar
- Verri WA Jr, Souto FO, Vieira SM, Almeida SC, Fukada SY, Xu D, Alves-Filho JC, Cunha TM, Guerrero AT, Mattos-Guimaraes RB, Oliveira FR, Teixeira MM, Silva JS, McInnes IB, Ferreira SH, Louzada-Junior P, Liew FY, Cunha FQ (2010) IL-33 induces neutrophil migration in rheumatoid arthritis and is a target of anti-TNF therapy. Ann Rheum Dis 69:1697–1703PubMedCrossRefGoogle Scholar