A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
- 370 Downloads
The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice.
Materials and methods
The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed.
A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue.
Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.
KeywordsEugenia malaccensis Lectin Antibacterial Cutaneous wound healing
This paper was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), FACEPE and CAPES, Brazil. The authors are deeply grateful for the technical assistance of Maria Barbosa Reis da Silva and João Antonio Virgínio and Alfa/VALNATURA Project.
Conflict of interest
The authors declare that they have no conflict of interest.
- Courvalin P, Goldstein F, Philippon A, Sirot J (1985) L’antibiogramme MPC. Vigot, Bruxelles, pp 191–195Google Scholar
- Hall LW, Clarke KW (1991) Veterinary anaesthesia, 9th edn. Ballière Tindall, LondonGoogle Scholar
- Morton J, Malay A (1987) Fruits of warm climates. Julia F. Morton, Miami, pp 378–381Google Scholar
- Peumans WJ, Van Damme EJM (1998) Plant lectins versatile proteins with important perspectives in biotechnology. Biotechnol Genet Eng Rev 15:199–228Google Scholar
- Spector M (2001) Biomaterials. In: Achauer B, Eriksson E, Guyuron B (eds) Plastic surgery, indications, operations, outcomes. Mosby Year Book, pp 239–259Google Scholar
- Whistler Polynesian Herbal Medicine (1992) Everbest Publishing Co., Hong KongGoogle Scholar