, Volume 18, Issue 2, pp 65–71 | Cite as

Synergism between COX-3 inhibitors in two animal models of pain

  • J. Muñoz
  • C. Navarro
  • V. Noriega
  • G. Pinardi
  • F. Sierralta
  • J. C. Prieto
  • H. F. Miranda
Research Article


Objective and design

The antinociception induced by the intraperitoneal coadministration in mice of combinations of metamizol and paracetamol was evaluated in the tail flick test and orofacial formalin test.


The antinociception of each drugs alone and the interaction of the combinations was evaluated by isobolographic analysis in the tail-flick and in the formalin orofacial assay of mice.


Mice pretreated with the drugs demonstrated that the antinociception of metamizol and paracetamol is dose-dependent. The potency range on the antinocifensive responses for metamizol or paracetamol was as follows: orofacial (Phase II) > orofacial (Phase I) > tail flick. In addition, the coadministration of metamizol with paracetamol induced a strong synergistic antinociception in the algesiometer assays. Both drugs showed effectiveness in inflammatory pain.


These actions can be related to the differential selectivity of the drugs for inhibition of COX isoforms and also to the several additional antinociception mechanisms and pathways initiated by the analgesic drugs on pain transmission. Since the efficacy of the combination of metamizol with paracetamol has been demonstrated in the present study, this association could have a potential beneficial effect on the pharmacological treatment of clinical pain.


Analgesia Synergism Pain Metamizol Paracetamol 



The expert technical assistance of José López and Alejandro Correa is gratefully acknowledged.


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Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2010

Authors and Affiliations

  • J. Muñoz
    • 1
  • C. Navarro
    • 1
  • V. Noriega
    • 2
  • G. Pinardi
    • 1
  • F. Sierralta
    • 1
  • J. C. Prieto
    • 1
    • 3
  • H. F. Miranda
    • 1
  1. 1.Pharmacology Program, Faculty of Medicine, ICBMUniversidad de ChileSantiagoChile
  2. 2.Faculty of Medicine, School of PharmacyUniversidad Andrés BelloSantiagoChile
  3. 3.Cardiovascular Department, Hospital ClínicoUniversidad de ChileSantiagoChile

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