Sensitive Spectroscopic Analysis of Biomarkers in Exhaled Breath

  • A. Bicer
  • J. Bounds
  • F. Zhu
  • A. A. Kolomenskii
  • N. Kaya
  • E. Aluauee
  • M. Amani
  • H. A. Schuessler
Part of the following topical collections:
  1. ICPPP-19: Selected Papers of the 19th International Conference on Photoacoustic and Photothermal Phenomena


We have developed a novel optical setup which is based on a high finesse cavity and absorption laser spectroscopy in the near-IR spectral region. In pilot experiments, spectrally resolved absorption measurements of biomarkers in exhaled breath, such as methane and acetone, were carried out using cavity ring-down spectroscopy (CRDS). With a 172-cm-long cavity, an efficient optical path of ~ 132 km was achieved. The CRDS technique is well suited for such measurements due to its high sensitivity and good spectral resolution. The detection limits for methane of ~ 8 ppbv and acetone of ~ 2.1 ppbv with spectral sampling of 0.005 cm−1 were achieved, which allowed to analyze multicomponent gas mixtures and to observe absorption peaks of 12CH4 and 13CH4. Further improvements of the technique have the potential to realize diagnostics of health conditions based on a multicomponent analysis of breath samples.


Absorption spectroscopy Breath analysis Cavity ring-down 



This work was supported by the Robert A. Welch Foundation Grant No. A1546 and the Qatar Foundation under the Grant NPRP 8-735-1-154. AB thanks for the support of the Turkish Ministry of Education, and EA thanks for the support of the Higher Committee for Educational Development in Iraq (HCED).


  1. 1.
    A. Amann, W. Miekisch, J. Schubert, B. Buszewski, T. Ligor, T. Jezierski, J. Pleil, T. Risby, Annu. Rev. Anal. Chem. 7, 455 (2014)CrossRefGoogle Scholar
  2. 2.
    M. Phillips, J. Herrera, S. Krishnan, M. Zain, J. Greenberg, R.N. Cataneo, J. Chromatogr. B 729, 75 (1999)CrossRefGoogle Scholar
  3. 3.
    M. Phillips, K. Gleeson, J.M.B. Hughes, J. Greenberg, R.N. Cataneo, L. Baker, W.P. McVay, Lancet 353, 1930 (1999)CrossRefGoogle Scholar
  4. 4.
    W. Cao, Y. Duan, Clin. Chem. 52, 800 (2006)CrossRefGoogle Scholar
  5. 5.
    B. Buszewski, M. Kesy, T. Ligor, A. Amann, Biomed. Chromatogr. 21, 553 (2007)CrossRefGoogle Scholar
  6. 6.
    R.A. Dweik, A. Amann, J. Breath Res. 2, 030301 (2008)ADSCrossRefGoogle Scholar
  7. 7.
    K.M. Paschke, A. Mashir, R.A. Dweik, Med. Rep. 2, 56 (2010)Google Scholar
  8. 8.
    C. Wang, P. Sahay, Sensors 9, 8230 (2009)CrossRefGoogle Scholar
  9. 9.
    J. Wojtas, Z. Bielecki, T. Stacewicz, J. Mikolajczyk, M. Nowakowski, Opto Electron. Rev. 20, 77 (2012)CrossRefGoogle Scholar
  10. 10.
    M.J. Navas, A.M. Jiménez, A.G. Asuero, Clin. Chim. Acta 413, 1171 (2012)CrossRefGoogle Scholar
  11. 11.
    Z.Y. Gong, M.X. Sun, C.Y. Jiang, Z.N. Wang, M.L. Kang, Y.X. Li, C. Wang, J. Anal. Bioanal. Tech. S7, 013 (2014)Google Scholar
  12. 12.
    R. Polikar, R. Shinar, V. Honavar, L. Upda, M. D. Porter, in Proceedings of the IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP), vol 5 (Salt Lake City, US, 8–11 May 2001), p. 3137 (2001)Google Scholar
  13. 13.
    C. Di Natale, A. Macagnano, E. Martinelli, R. Paolesse, G. D’Arcangelo, C. Roscioni, A. Finazzi-Agro, A. D’Amico, Biosens. Bioelectron. 18, 1209–1218 (2003)CrossRefGoogle Scholar
  14. 14.
    M. Fleischer, E. Simon, E. Rumpel, H. Ulmer, M. Harbeck, M. Wandel, C. Fietzek, U. Weimar, H. Meixner, Sens. Actuators B 83, 245–249 (2002)CrossRefGoogle Scholar
  15. 15.
    A. Anton, A. Agapiou, in 4th International Conference on Wireless Mobile Communication and Healthcare—Transforming Healthcare Through Innovations in Mobile and Wireless Technologies (MOBIHEALTH 2014), Athens, 2014, p. 250Google Scholar
  16. 16.
    M. McCurdy, Y. Bakhirkin, G. Wysocki, F.K. Tittel, J. Breath Res. 1, 014 001 (2007)CrossRefGoogle Scholar
  17. 17.
    M.J. Thorpe, D. Balslev-Clausen, M.S. Kirchner, J. Ye, Opt. Express 16, 2387 (2008)ADSCrossRefGoogle Scholar
  18. 18.
    T.H. Risby, F.K. Tittel, Opt. Eng. 49, 111123 (2010)ADSCrossRefGoogle Scholar
  19. 19.
    D.D. Arslanov, K. Swinkels, S.M. Cristescu, F.J.M. Harren, Opt. Express 19, 24078 (2011)ADSCrossRefGoogle Scholar
  20. 20.
    J.C. Anderson, W.J.E. Lamm, M.P. Hlastala, J. Appl. Physiol. 100, 880 (2006)CrossRefGoogle Scholar
  21. 21.
    P.J. Mazzone, J. Breath Res. 6, 027106 (2012)ADSCrossRefGoogle Scholar
  22. 22.
    U. Tisch, S. Billan, M. Ilouze, M. Phillips, N. Peledand, H. Haick, CML Lung Cancer 5, 107 (2012)Google Scholar
  23. 23.
    D.F. Altomare, M.D. Lena, F. Porcelli, L. Trizio, E. Travaglio, M. Tutino, S. Dragonieri, V. Memeo, G. Gennaro, Br. J. Surg. 100, 144 (2013)CrossRefGoogle Scholar
  24. 24.
    M. Hakim, S. Billan, U. Tisch, G. Peng, I. Dvrokind, O. Marom, R. Abdah-Bortnyak, A. Kuten, H. Haick, Br. J. Cancer 104, 1649 (2011)CrossRefGoogle Scholar
  25. 25.
    M.W. Sigrist, R. Bartlome, D. Marinov, J.M. Rey, D.E. Vogler, H. Wächter, Appl. Phys. B 90, 289 (2008)ADSCrossRefGoogle Scholar
  26. 26.
    K. Namjou, C.B. Roller, G. McMillen, in Proceedings of the 6th Annual IEEE Conference on Sensors, Atlanta, GA, USA (2007), p. 1337Google Scholar
  27. 27.
    C. Roller, K. Namjou, J. Jeffers, W. Potter, P.J. McCann, J. Grego, Opt. Lett. 27, 107 (2002)ADSCrossRefGoogle Scholar
  28. 28.
    M. Mazurenka, A.J. Orr-Ewing, R. Peverall, G.A.D. Ritchie, Annu. Rep. Prog. Chem. Sect. C Phys. Chem. 101, 100 (2005)CrossRefGoogle Scholar
  29. 29.
    L.R. Narasimhan, W. Goodman, C.K.N. Patel, Proc. Natl. Acad. Sci. USA 98, 4617 (2001)ADSCrossRefGoogle Scholar
  30. 30.
    Y.A. Bakhirkin, A.A. Kosterev, C. Roller, R.F. Curl, F.K. Tittel, Appl. Opt. 43, 2257 (2004)ADSCrossRefGoogle Scholar
  31. 31.
    H. Dahnke, D. Kleine, P. Hering, M. Mürtz, Appl. Phys. B 72, 971 (2001)ADSCrossRefGoogle Scholar
  32. 32.
    A. Kosterev, YuA Bakhirkin, R.F. Curl, F.K. Tittel, Opt. Lett. 27, 1902 (2002)ADSCrossRefGoogle Scholar
  33. 33.
    P. Patimisco, G. Scamarcio, F.K. Tittel, V. Spagnolo, Sensors 14, 6165 (2014)CrossRefGoogle Scholar
  34. 34.
    J. Xia, F. Zhu, S. Zhang, A. Kolomenskii, H. Schuessler, Infrared Phys. Technol. 86, 194 (2017)ADSCrossRefGoogle Scholar
  35. 35.
    G. Danaei, M.M. Finucane, Y. Lu, G.M. Singh, M.J. Cowan, C.J. Paciorek, J.K. Lin, F. Farzadfar, Y.H. Khang, G.A. Stevens, M. Rao, Lancet 378, 31 (2011)CrossRefGoogle Scholar
  36. 36.
    O.E. Owen, V.E. Trapp, C.L. Skutches, M.A. Mozzoli, R.D. Hoeldtke, G. Boden, G.A. Reichard, J. Diabetes 31, 242 (1982)CrossRefGoogle Scholar
  37. 37.
    A.W. Jones, J. Anal. Toxicol. 24, 8 (2000)CrossRefGoogle Scholar
  38. 38.
    Z. Wang, C. Wang, J. Breath Res. 7, 037109 (2013)ADSCrossRefGoogle Scholar
  39. 39.
    T.H. Risby, J.D. Pleil, J. Breath Res. 7, 010201 (2013)ADSCrossRefGoogle Scholar
  40. 40.
    C. Wang, S.T. Scherrer, D. Hossain, Appl. Spectrosc. 58, 784 (2004)ADSCrossRefGoogle Scholar
  41. 41.
    M. Sun, C. Jiang, Z. Gong, X. Zhao, Z. Chen, Z. Wang, M. Kang, Y. Li, C. Wang, Rev. Sci. Instrum. 86, 095003 (2015)ADSCrossRefGoogle Scholar
  42. 42.
    S. Halbritter, M. Fedrigo, V. Höllriegl, W. Szymczak, J.M. Maier, A.G. Ziegler, M. Hummel, Diabetes Technol. Ther. 14, 917 (2012)CrossRefGoogle Scholar
  43. 43.
    L.L. Marchand, L.R. Wilkens, P. Harwood, R.V. Cooney, Int. J. Cancer 55, 887 (1993)CrossRefGoogle Scholar
  44. 44.
    L. Le Marchand, L.R. Wilkens, P. Harwood, R.V. Cooney, Environ. Health Perspect. 98, 199 (1992)CrossRefGoogle Scholar
  45. 45.
    Y. Chen, K.K. Lehmann, J. Kessler, B.S. Lollar, G.L. Couloume, T.C. Onstott, Anal. Chem. 85, 11250 (2013)CrossRefGoogle Scholar
  46. 46.
    C. Jiang, M. Sun, Z. Wang, Z. Chen, X. Zhao, Y. Yuan, Y. Li, C. Wang, Sensors 16, 1199 (2016)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • A. Bicer
    • 1
  • J. Bounds
    • 1
  • F. Zhu
    • 1
  • A. A. Kolomenskii
    • 1
  • N. Kaya
    • 2
    • 3
  • E. Aluauee
    • 1
  • M. Amani
    • 4
  • H. A. Schuessler
    • 1
    • 2
  1. 1.Department of PhysicsTexas A&M UniversityCollege StationUSA
  2. 2.Science DepartmentTexas A&M University at QatarDohaQatar
  3. 3.Department of Physics, Faculty of Arts and SciencesGiresun UniversityGiresunTurkey
  4. 4.Petroleum Engineering ProgramTexas A&M University at QatarDohaQatar

Personalised recommendations