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Emodin Attenuates Severe Acute Pancreatitis via Antioxidant and Anti-inflammatory Activity

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Abstract

There is no specific drug to treat severe acute pancreatitis (SAP), which induces substantial medical and social burden. Many studies have reported the beneficial effects of emodin against SAP in vivo and in vitro. However, the underlying mechanism has been unclear. This paper described the design and implementation of anti-inflammatory and antioxidant activity of emodin. Emodin restored the pathological damage of SAP and simultaneously decreased the high levels of serum amylase, lipase, TNF-α, and IL-18 in the peripheral blood of SAP rat. Emodin reversed reactive oxygen species (ROS) in neutrophils derived from SAP rat. The levels of voltage-dependent anion channel 1 (VDAC1), NOD-like receptor protein 3 (NLRP3), caspase-1, and IL-18 were examined to analyze the change of inflammasome-related mediators between SAP and emodin treatment. These findings suggest that emodin plays its protective role on SAP against oxidative stress and inflammasome signals.

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Acknowledgments

We wish to acknowledge Xue Sui for the help in the flow cytometry assay and Xiaoxin Sun for the arrangement of the daily work in the laboratory.

Funding

This research was supported by the National Natural Science Foundation of China (No. 81873156 and No. 81703871), Key Project Supported by Clinical Ability Construction of Liaoning Province (No. LNCCC-A03-2015), and Doctoral Start-up Foundation of Liaoning Province (No. 20170520408).

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Author notes

  1. Shilin Xia and Yujia Ni contributed equally to this work.

Authors and Affiliations

  1. Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, No. 222 Zhongshan Road, Dalian, China

    Shilin Xia & Hong Xiang

  2. Department of Geriatrics, Shaoxing People’s Hospital, No. 568, Zhongxing North Road, Shaoxing, Zhejiang, China

    Yujia Ni

  3. Institute (College) of Integrative Medicine, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian, China

    Qi Zhou, Hua Sui & Dong Shang

  4. Department of Oral Pathology, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian, China

    Han liu

  5. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, No. 222 Zhongshan Road, Dalian, China

    Dong Shang

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  1. Shilin Xia
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Contributions

Shilin Xia and Dong Shang participated in the design of this study. Shilin Xia performed the manuscript review. Yujia Ni exerted the main experiment and performed the statistical analysis. Shilin Xia and Han Liu drafted the manuscript. Qi Zhou, Hong Xiang, and Hua Sui provided assistance for the data acquisition and data analysis. All authors read and approved the final manuscript.

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Correspondence to Dong Shang.

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Xia, S., Ni, Y., Zhou, Q. et al. Emodin Attenuates Severe Acute Pancreatitis via Antioxidant and Anti-inflammatory Activity. Inflammation 42, 2129–2138 (2019). https://doi.org/10.1007/s10753-019-01077-z

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