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Chikusetsusaponin V Inhibits LPS-Activated Inflammatory Responses via SIRT1/NF-κB Signaling Pathway in RAW264.7 Cells

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Abstract

It is becoming increasingly accepted that macrophage activation play a crucial role in many diseases associated with chronic inflammation, such as metabolic disease, including atherosclerosis, obesity, and diabetes. Recent studies have indicated that the regulation of inflammation and energy metabolism are connected together through an antagonistic crosstalk between NF-κB and SIRT1 signaling pathways. In order to investigate anti-inflammatory drugs, we investigated whether SIRT1 is implicated in the lipopolysaccharide (LPS)-stimulated NF-κB signaling pathway in macrophage RAW264.7 cells when pretreated with chikusetsusaponin V (CsV). Griess assay and enzyme-linked immunosorbent assay were used to determine the effect of CsV on LPS-induced inflammatory cytokine secretion. Western blot was used to assess the effect of CsV on LPS-induced SIRT1 and Ac-NF-κB p65 expression. Results showed that CsV suppressed LPS-induced inflammatory cytokine NO, TNF-α, and IL-1β production in RAW264.7 cells. In addition, downregulation of SIRT1 and upregulation of Ac-NF-κB p65 induced by LPS were abolished by CsV in a dose-dependent manner in RAW264.7 cells. Meanwhile, inflammatory cytokines were regulated with CsV through SIRT1-Ac-NF-κB p65 signaling pathway. Therefore, our results demonstrate that CsV exerts an anti-inflammatory effect partly through SIRT1/NF-κB signaling pathways and SIRT1 may be a new target for anti-inflammation therapies.

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Acknowledgments

The authors thank the College of Medical Science of Three Gorges University for their good facilities and all the laboratory workers who have helped with the methods and materials.

Funding

This study was financed by the grants from the National Natural Science Foundation of China (NSFC) (No. 31570329) to Ding Yuan.

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Author notes

  1. Qin Yuan contributed equally to this work.

Authors and Affiliations

  1. Affiliated Renhe Hospital of Three Gorges University, Yichang, 443001, Hubei, China

    Qin Yuan & Ding Yuan

  2. College of Medical Science, Three Gorges University, Yichang, 443002, Hubei, China

    Qin Yuan, Dengqing Zhang, Chaoqi Liu, Changcheng Zhang & Ding Yuan

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  1. Qin Yuan
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  2. Dengqing Zhang
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Correspondence to Ding Yuan.

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Yuan, Q., Zhang, D., Liu, C. et al. Chikusetsusaponin V Inhibits LPS-Activated Inflammatory Responses via SIRT1/NF-κB Signaling Pathway in RAW264.7 Cells. Inflammation 41, 2149–2159 (2018). https://doi.org/10.1007/s10753-018-0858-8

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  • DOI: https://doi.org/10.1007/s10753-018-0858-8

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