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High-Intensity Exercise Prevents Disturbances in Lung Inflammatory Cytokines and Antioxidant Defenses Induced by Lipopolysaccharide

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Abstract

In this study, we evaluated the effects of high-intensity swimming in an experimental model of acute lung injury (ALI) induced by lipopolysaccharide (LPS) on lung inflammation and antioxidant defenses. Balb/C male mice were submitted to exercise (30 min/day, 5 days/week, for a period of 3 weeks) prior to LPS instillation in the lung. Twenty-four hours after delivery of LPS (10 μg/animal), mice were euthanized and bronchoalveolar fluid (BALF) was obtained for cell counting and analysis of cytokines by ELISA. Lung tissue was used to evaluate antioxidant defenses. LPS instillation resulted in an increase in total and mononuclear cells, IL-1β, TNF-α, and IL-6 in BALF. LPS instillation also altered IL-10 and IL-ra levels in BALF and induced antioxidant defenses (glutathione, superoxide dismutase, catalase, and glutathione peroxidase) in the lung. Protein carbonyl increased in the LPS-treated animals. High-intensity swimming prevented all these changes induced by LPS. Significance: Therefore, this experimental protocol of high-intensity swimming showed a protective effect on ALI, decreasing inflammatory processes and preventing disturbances in antioxidant defenses into the lungs.

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Funding

This work was supported by the State University of Santa Catarina and Research Support Foundation of Santa Catarina.

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Authors and Affiliations

  1. Department of Physical Therapy, State University of Santa Catarina, Florianopolis, Brazil

    Gisele Henrique Cardoso, Débora Melissa Petry, Jéssica Jorge Probst & Deborah de C. Hizume Kunzler

  2. Experimental Research Laboratory, LaPEx, R. Pascoal Simone, 358, Coqueiros, Florianópolis, Santa Catarina, Brazil

    Gisele Henrique Cardoso, Débora Melissa Petry, Jéssica Jorge Probst, Kelly Cattelan Bonorino & Deborah de C. Hizume Kunzler

  3. Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, Brazil

    Luiz Felipe de Souza, Gabriella Ganguilhet & Alcir Luiz Dafre

  4. Postgraduate Program in Health Science, University of Southern Santa Catarina, Florianopolis, Santa Catarina, Brazil

    Franciane Bobinski & Daniel Fernandes Martins

  5. Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil

    Adair R. S. Santos & Kelly Cattelan Bonorino

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  1. Gisele Henrique Cardoso
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  2. Débora Melissa Petry
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  7. Adair R. S. Santos
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  11. Deborah de C. Hizume Kunzler
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Correspondence to Deborah de C. Hizume Kunzler.

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This study was approved by the Review Board for Animal Studies of the Federal University of Santa Catarina (PP 00745). All animals in this study received humane care in compliance with EU Directive 2010/63/EU, which provides guidelines for animal experiments.

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Cardoso, G.H., Petry, D.M., Probst, J.J. et al. High-Intensity Exercise Prevents Disturbances in Lung Inflammatory Cytokines and Antioxidant Defenses Induced by Lipopolysaccharide. Inflammation 41, 2060–2067 (2018). https://doi.org/10.1007/s10753-018-0849-9

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