, Volume 41, Issue 2, pp 722–731 | Cite as

ZEB2 Attenuates LPS-Induced Inflammation by the NF-κB Pathway in HK-2 Cells

  • Qi Ding
  • Yang Wang
  • Ai-ling Zhang
  • Tao Xu
  • Dan-dan Zhou
  • Xiao-Feng Li
  • Jun-Fa Yang
  • Lei Zhang
  • Xiao Wang


As a transcription factor, zinc finger E-box binding homeobox 2 (ZEB2) includes multiple functional domains which interact with kinds of transcriptional co-effectors. It has been reported that ZEB2 was involved in signal transduction and multiple cellular functions. However, the functional role of ZEB2 in inflammation is still obscure. The aim of the current study is to explore the function of ZEB2 in inflammation cytokine secretion and the role of the nuclear factor-κB (NF-κB) signaling pathway in lipopolysaccharide (LPS)-induced human proximal tubule cell line (HK-2) cells. Our result demonstrated that expression of ZEB2 was significantly downregulated and expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was upregulated in response to LPS. Meanwhile, knockdown of ZEB2 by transfecting siRNA increased TNF-α and IL-6 secretion. Overexpression of ZEB2 resulted in a decrease of TNF-α and IL-6 secretion in HK-2 cells. Additionally, Western blot analysis indicated that ZEB2 suppressed the activation of the NF-κB signaling pathway via downregulating the levels of phosphorylated p65 and IκBα compared with LPS stimulation. Collectively, our data demonstrated that ZEB2 attenuated LPS-induced inflammation cytokine secretion possibly through suppressing the NF-κB signaling pathway.


ZEB2 TNF-α IL-6 NF-κB signaling pathway 



Acute kidney injury


Dulbecco’s modified Eagle’s medium


Epithelial-mesenchymal transition

HK-2 cell

Human kidney epithelial cell






Nuclear factor-κB


Polymerase chain reaction


Phosphorylated p65


Phosphorylated IκBα


Pyrrolidine dithiocarbamate


Small interfering RNA


Transforming growth factor beta 1


Tumor necrosis factor-α


Zinc finger E-box-binding homeobox 2



This study was supported by the Chinese National Natural Science Foundation Project (81100302) and the Intercollegiate Key Projects of Nature Science of Anhui Province (KJ2017A169).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Fig. S1

(GIF 54 kb)

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High resolution image (TIFF 435 kb)
10753_2017_727_MOESM2_ESM.pdf (122 kb)
ESM 2 (PDF 122 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Qi Ding
    • 1
    • 2
    • 3
  • Yang Wang
    • 1
    • 2
    • 3
  • Ai-ling Zhang
    • 1
    • 2
    • 3
  • Tao Xu
    • 1
    • 2
    • 3
  • Dan-dan Zhou
    • 1
    • 2
    • 3
  • Xiao-Feng Li
    • 1
    • 2
    • 3
  • Jun-Fa Yang
    • 1
    • 2
    • 3
  • Lei Zhang
    • 1
    • 2
    • 3
  • Xiao Wang
    • 4
  1. 1.School of PharmacyAnhui Medical UniversityHefeiChina
  2. 2.Key Laboratory of Major Autoimmune Disease, Anhui Province; School of PharmacyAnhui Medical UniversityHefeiChina
  3. 3.The Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of EducationAnhui Medical UniversityHefeiChina
  4. 4.Department of RadiologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina

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