, Volume 41, Issue 2, pp 569–578 | Cite as

Overexpression of SIRT2 Alleviates Neuropathic Pain and Neuroinflammation Through Deacetylation of Transcription Factor Nuclear Factor-Kappa B

  • Yong Zhang
  • Dachao Chi


Sirtuin 2 (SIRT2), a member of the mammalian sirtuin family, plays an important role in the pathogenesis of various neurological diseases. However, whether SIRT2 is involved in the regulation of neuropathic pain remains unclear. In this study, we aimed to investigate the potential role of SIRT2 in regulating neuropathic pain in a rat model induced by chronic constriction injury (CCI). We found that SIRT2 was downregulated in the dorsal root ganglion (DRG) in CCI rats. Intrathecal injection of a recombinant adenovirus expressing SIRT2 markedly alleviated mechanical allodynia and thermal hyperalgesia in CCI rats. This also inhibited the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 in the DRG of CCI rats. Moreover, our results showed that overexpression of SIRT2 inhibited the acetylation of the nuclear factor-kappa B (NF-κB) p65 protein in the DRG of CCI rats. Additionally, treatment with a SIRT2 specific inhibitor significantly aggravated neuropathic pain and attenuated the inhibitory effect of SIRT2 overexpression on neuropathic pain development. Taken together, these results suggest that overexpression of SIRT2 alleviates neuropathic pain associated with inhibition of NF-κB signaling and neuroinflammation. Therefore, SIRT2 may serve as a potential therapeutic target for treatment of neuropathic pain.

Key Words

chronic constriction injury neuropathic pain NF-κB SIRT2 



Sirtuin 2


Chronic constriction injury


Dorsal root ganglion


Tumor-necrosis factor-α




Nuclear factor-kappa B


Forkhead box O1


Forkhead box 3a




Real-time quantitative polymerase chain reaction


Glyceraldehyde-3-phosphate dehydrogenase


Bicinchoninic acid


Sodium dodecylsulfate-polyacrylamide gel electrophoresis


Enzyme-linked immunosorbent assay


Paw withdrawal latencies


Paw withdrawal threshold


Compliance with Ethical Standards

This study was reviewed and approved by the Institutional Animal Care and Use Committee of Shaanxi Provincial People’s Hospital. The animal experiments were performed according to the guidelines of the International Association for the Study of Pain and the National Institute of Health Guide for the Care and Use of Laboratory Animals.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Department of AcupunctureShaanxi Provincial People’s HospitalXi’anChina
  2. 2.Department of General SurgeryShaanxi Provincial People’s HospitalXi’anChina

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