The aim of this study is to investigate the cardioprotective effects of morroniside in rats following acute myocardial infarction. An acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD) . Following AMI, morroniside was administered intragastrically for 24 h at doses of 45, 90, and 180 mg/kg, respectively. Biomarkers such as creatine kinase (CK-MB), lactate dehydrogenase (LDH), ɑ-hydroxybutyrate dehydrogenase (ɑ-HBDH), and aspartate aminotransferase (AST) activities in AMI rats in the serum were detected with commercial kits . Following AMI, morroniside was administered intragastrically for 72 h at doses of 45, 90, and 180 mg/kg/d, respectively. The expression of nuclear factor kappa B (NF-κB) in cardiac myocardium was detected by western blotting analysis. Meanwhile, cardiac function was measured by echocardiography. We observed morroniside decreased the levels of CK-MB, LDH, ɑ-HBDH, and AST activities in AMI rats after 24 h. We also found that morroniside reduced the expression of NF-κB in cardiac myocardium at 72 h post AMI rats. Further, cardiac function was improved by administration of morroniside. Collectively, our findings demonstrated that morroniside had cardioprotective effects in rats following acute myocardial infarction. Attenuation of inflammation might contribute to the cardioprotective effects of morroniside.
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This study was supported by the National Science and Technology Major Project (2012ZX09102201-106); National Natural Science Foundation of China (81373994, 81573633, 81503049, 81173575).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
Lin, D., J. Ma, Y. Xue, and Z. Wang. 2015. Penehyclidine hydrochloride preconditioning provides cardioprotection in a rat model of myocardial ischemia/reperfusion injury. PLoS One 10: e138051.Google Scholar
Ya, B., C. Li, L. Zhang, W. Wang, and L. Li. 2010. Cornel iridoid glycoside inhibits inflammation and apoptosis in brains of rats with focal cerebral ischemia. Neurochemical Research 35 (5): 773–781.CrossRefPubMedGoogle Scholar
Deng, X., J. Chen, H. Li, Z. Ma, S. Ma, and Q. Fu. 2015. Cardioprotective effects of timosaponin B II from Anemarrhenae asphodeloides Bge on isoproterenol-induced myocardial infarction in rats. Chemico-Biological Interactions 240: 22–28.CrossRefPubMedGoogle Scholar
Wang, W., W. Huang, L. Li, et al. 2008. Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells. Cellular and Molecular Neurobiology 28 (2): 293–305.CrossRefPubMedGoogle Scholar
Wang, W., F. Sun, Y. An, et al. 2009. Morroniside protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide-induced cytotoxicity. European Journal of Pharmacology 613 (1–3): 19–23.CrossRefPubMedGoogle Scholar
Wang, W., J. Xu, L. Li, et al. 2010. Neuroprotective effect of morroniside on focal cerebral ischemia in rats. Brain Research Bulletin 83 (5): 196–201.CrossRefPubMedGoogle Scholar
Sun, F., W. Wang, W. Zuo, et al. 2014. Promoting neurogenesis via Wnt/β-catenin signaling pathway accounts for the neurorestorative effects of morroniside against cerebral ischemia injury. European Journal of Pharmacology 738: 214–221.CrossRefPubMedGoogle Scholar
Sun, F.L., W. Wang, H. Cheng, et al. 2014. Morroniside improves microvascular functional integrity of the neurovascular unit after cerebral ischemia. PLoS One 9: e101194.CrossRefPubMedCentralPubMedGoogle Scholar
D Uva, G., A. Aharonov, M. Lauriola, et al. 2015. ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation. Nature Cell Biology 17 (5): 627–638.CrossRefGoogle Scholar
Selye, H., E. Bajusz, S. Grasso, and P. Mendell. 1960. Simple techniques for the surgical occlusion of coronary vessels in the rat. Angiology 11: 398–407.CrossRefPubMedGoogle Scholar
Li, H., Y.H. Xie, Q. Yang, et al. 2012. Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats. PLoS One 7: e48872.CrossRefPubMedCentralPubMedGoogle Scholar
Hackel, D.B., K.A. Reimer, R.E. Ideker, E.M. Mikat, and T.D. Hartwell. 1984. Comparison of enzymatic and anatomic estimates of myocardial infarct size in man. Circulation 70 (5): 824–835.CrossRefPubMedGoogle Scholar
Guo, J., S. Wang, T. Yuan, et al. 2013. Coptisine protects rat heart against myocardial ischemia/reperfusion injury by suppressing myocardial apoptosis and inflammation. Atherosclerosis 231 (2): 384–391.CrossRefPubMedGoogle Scholar
Zhang, S., X. Liu, S. Goldstein, et al. 2013. Role of the JAK/STAT signaling pathway in the pathogenesis of acute myocardial infarction in rats and its effect on NF-κB expression. Molecular Medicine Reports 7 (1): 93–98.CrossRefPubMedGoogle Scholar
Yokozawa, T., K.S. Kang, C.H. Park, et al. 2010. Bioactive constituents of Corni Fructus: The therapeutic use of morroniside, loganin, and 7-O-galloyl-D-sedoheptulose as renoprotective agents in type 2 diabetes. Drug Discovery Therapy 4 (4): 223–234.Google Scholar
Mulvihill, N.T., and J.B. Foley. 2002. Inflammation in acute coronary syndromes. European Heart Journal Supplements: Journal of the European Society of Cardiology 88: 800–803.Google Scholar