, Volume 41, Issue 2, pp 432–436 | Cite as

Cardioprotective Effects of Morroniside in Rats Following Acute Myocardial Infarction

  • Bangxing Yu
  • Wen Wang


The aim of this study is to investigate the cardioprotective effects of morroniside in rats following acute myocardial infarction. An acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD) [1]. Following AMI, morroniside was administered intragastrically for 24 h at doses of 45, 90, and 180 mg/kg, respectively. Biomarkers such as creatine kinase (CK-MB), lactate dehydrogenase (LDH), ɑ-hydroxybutyrate dehydrogenase (ɑ-HBDH), and aspartate aminotransferase (AST) activities in AMI rats in the serum were detected with commercial kits [2]. Following AMI, morroniside was administered intragastrically for 72 h at doses of 45, 90, and 180 mg/kg/d, respectively. The expression of nuclear factor kappa B (NF-κB) in cardiac myocardium was detected by western blotting analysis. Meanwhile, cardiac function was measured by echocardiography. We observed morroniside decreased the levels of CK-MB, LDH, ɑ-HBDH, and AST activities in AMI rats after 24 h. We also found that morroniside reduced the expression of NF-κB in cardiac myocardium at 72 h post AMI rats. Further, cardiac function was improved by administration of morroniside. Collectively, our findings demonstrated that morroniside had cardioprotective effects in rats following acute myocardial infarction. Attenuation of inflammation might contribute to the cardioprotective effects of morroniside.


morroniside acute myocardial infarction (AMI) cardioprotection 



This study was supported by the National Science and Technology Major Project (2012ZX09102201-106); National Natural Science Foundation of China (81373994, 81573633, 81503049, 81173575).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Lin, D., J. Ma, Y. Xue, and Z. Wang. 2015. Penehyclidine hydrochloride preconditioning provides cardioprotection in a rat model of myocardial ischemia/reperfusion injury. PLoS One 10: e138051.Google Scholar
  2. 2.
    Ya, B., C. Li, L. Zhang, W. Wang, and L. Li. 2010. Cornel iridoid glycoside inhibits inflammation and apoptosis in brains of rats with focal cerebral ischemia. Neurochemical Research 35 (5): 773–781.CrossRefPubMedGoogle Scholar
  3. 3.
    Deng, X., J. Chen, H. Li, Z. Ma, S. Ma, and Q. Fu. 2015. Cardioprotective effects of timosaponin B II from Anemarrhenae asphodeloides Bge on isoproterenol-induced myocardial infarction in rats. Chemico-Biological Interactions 240: 22–28.CrossRefPubMedGoogle Scholar
  4. 4.
    Wang, W., W. Huang, L. Li, et al. 2008. Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells. Cellular and Molecular Neurobiology 28 (2): 293–305.CrossRefPubMedGoogle Scholar
  5. 5.
    Wang, W., F. Sun, Y. An, et al. 2009. Morroniside protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide-induced cytotoxicity. European Journal of Pharmacology 613 (1–3): 19–23.CrossRefPubMedGoogle Scholar
  6. 6.
    Wang, W., J. Xu, L. Li, et al. 2010. Neuroprotective effect of morroniside on focal cerebral ischemia in rats. Brain Research Bulletin 83 (5): 196–201.CrossRefPubMedGoogle Scholar
  7. 7.
    Sun, F., W. Wang, W. Zuo, et al. 2014. Promoting neurogenesis via Wnt/β-catenin signaling pathway accounts for the neurorestorative effects of morroniside against cerebral ischemia injury. European Journal of Pharmacology 738: 214–221.CrossRefPubMedGoogle Scholar
  8. 8.
    Sun, F.L., W. Wang, H. Cheng, et al. 2014. Morroniside improves microvascular functional integrity of the neurovascular unit after cerebral ischemia. PLoS One 9: e101194.CrossRefPubMedCentralPubMedGoogle Scholar
  9. 9.
    D Uva, G., A. Aharonov, M. Lauriola, et al. 2015. ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation. Nature Cell Biology 17 (5): 627–638.CrossRefGoogle Scholar
  10. 10.
    Selye, H., E. Bajusz, S. Grasso, and P. Mendell. 1960. Simple techniques for the surgical occlusion of coronary vessels in the rat. Angiology 11: 398–407.CrossRefPubMedGoogle Scholar
  11. 11.
    Li, H., Y.H. Xie, Q. Yang, et al. 2012. Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats. PLoS One 7: e48872.CrossRefPubMedCentralPubMedGoogle Scholar
  12. 12.
    Hackel, D.B., K.A. Reimer, R.E. Ideker, E.M. Mikat, and T.D. Hartwell. 1984. Comparison of enzymatic and anatomic estimates of myocardial infarct size in man. Circulation 70 (5): 824–835.CrossRefPubMedGoogle Scholar
  13. 13.
    Guo, J., S. Wang, T. Yuan, et al. 2013. Coptisine protects rat heart against myocardial ischemia/reperfusion injury by suppressing myocardial apoptosis and inflammation. Atherosclerosis 231 (2): 384–391.CrossRefPubMedGoogle Scholar
  14. 14.
    Wu, X., L. He, F. Chen, et al. 2014. Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction. PLoS One 9: e112891.CrossRefPubMedCentralPubMedGoogle Scholar
  15. 15.
    Zhang, S., X. Liu, S. Goldstein, et al. 2013. Role of the JAK/STAT signaling pathway in the pathogenesis of acute myocardial infarction in rats and its effect on NF-κB expression. Molecular Medicine Reports 7 (1): 93–98.CrossRefPubMedGoogle Scholar
  16. 16.
    Yokozawa, T., K.S. Kang, C.H. Park, et al. 2010. Bioactive constituents of Corni Fructus: The therapeutic use of morroniside, loganin, and 7-O-galloyl-D-sedoheptulose as renoprotective agents in type 2 diabetes. Drug Discovery Therapy 4 (4): 223–234.Google Scholar
  17. 17.
    Mulvihill, N.T., and J.B. Foley. 2002. Inflammation in acute coronary syndromes. European Heart Journal Supplements: Journal of the European Society of Cardiology 88: 800–803.Google Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Experimental Animal CentreXuanWu Hospital of Capital Medical UniversityBeijingPeople’s Republic of China

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