Inflammation

, Volume 41, Issue 2, pp 368–377 | Cite as

Characteristic Comparison of Meningitis and Non-meningitis of Streptococcus suis in an Experimentally Infected Porcine Model

  • Yingying Sun
  • Hongtao Liu
  • Rui Du
  • Shuguang Li
  • Guanggang Qu
  • Rining Zhu
  • Shuang Zhao
  • Jingmin Gu
  • Changjiang Sun
  • Xin Feng
  • Wenyu Han
  • Liancheng Lei
ORIGINAL ARTICLE

Abstract

This study tested the differences of meningitis and non-meningitis of Streptococcus suis (SS). In this study, an infected pig model of streptococcal meningitis was established. Compared with the non-meningitis Streptococcus suis group (JZLQ001 group), the meningitis Streptococcus suis group (JZLQ022) exhibited neurological symptoms, such as ataxia and foaming at the mouth, and the brain showed a large area of congestion at 5 days post-infection (p.i.). Moreover, bacterial counts, white blood cells (WBCs), neutrophils, and blood glucose in the blood reached a peak and were significantly higher than those of the JZLQ001 group at 3 days p.i. These values then decreased at 5 days p.i. However, the content of total protein in the blood was lower in the JZLQ022 group than that in the JZLQ001 group, and the difference was most significant at 5 days p.i. When neurological symptoms appeared on 5 days p.i., the bacterial counts in the brain in the JZLQ022 group were significantly higher than those in the JZLQ001 group. The levels of cytokines in the peripheral blood and cerebrospinal fluid (CSF) were an important indicator of inflammation. By ELISA detection, the secretion levels of IL-6, IL-8, and IL-17 in the peripheral blood in the JZLQ022 group were significantly higher than those in the JZLQ001 group at 12 and 24 h and 3 days p.i.; however, TNF-α showed no difference. At 5 days p.i., the secretion levels of IL-6, IL-8, and IL-17 in the JZLQ022 group were significantly lower than those in the JZLQ001 group. The results were similar in CSF. HE staining revealed that the JZLQ022 group exhibited neuronophagia and hyperemia in the brain, but no change was found in the JZLQ001 group. A further study investigating the impact of meningitis Streptococcus suis on blood-brain barrier (BBB) integrity found that the brain tissue content of endogenous IgG in the JZLQ022 group was significantly higher than that in the JZLQ001 group. The present study demonstrated that pigs infected with meningitis and non-meningitis Streptococcus suis exhibit significant differences in immunological aspects such as bacterial counts, WBCs, neutrophils, blood glucose and total protein in the peripheral blood, the secretion levels of IL-6, IL-8, and IL-17, and BBB integrity. These data provide the necessary evidence to better understand SS meningitis.

KEY WORDS

Streptococcus suis meningitis blood-brain barrier immunological aspects 

ABBREVIATIONS

SS

Streptococcus suis

MCP

Monocyte chemotaxis proteins

CSF

Cerebrospinal fluid

WBCs

White blood cells

PBS

Phosphate-buffered saline

BBB

Blood-brain barrier

Notes

ACKNOWLEDGEMENTS

This study was supported by “The National Key R&D Program of China” (2017YPD0500204).

AUTHOR CONTRIBUTIONS

YS and LL conceived and designed the experiments. YS, HL, RD, SL, GQ, and RZ performed the experiments. YS analyzed the data. SZ, JG, CS, XF, and WH contributed reagents/materials/analysis tools. YS contributed to the writing of the manuscript. All authors read and approved the final manuscript.

COMPLIANCE WITH ETHICAL STANDARDS

CONFLICT OF INTEREST

The authors declare that they have no competing interests.

ETHICAL APPROVAL

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The protocol was reviewed by the Institutional Animal Care and the Committee of Jilin University under the approved protocol number JLUA-1309.

INFORMED CONSENT

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Yingying Sun
    • 1
  • Hongtao Liu
    • 1
  • Rui Du
    • 2
  • Shuguang Li
    • 3
  • Guanggang Qu
    • 3
  • Rining Zhu
    • 1
  • Shuang Zhao
    • 1
  • Jingmin Gu
    • 1
  • Changjiang Sun
    • 1
  • Xin Feng
    • 1
  • Wenyu Han
    • 1
  • Liancheng Lei
    • 1
  1. 1.College of Veterinary MedicineJilin UniversityChangchunPeople’s Republic of China
  2. 2.College of Chinese Medicine MaterialJilin Agricultural UniversityChangchunPeople’s Republic of China
  3. 3.Shandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhouPeople’s Republic of China

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