, Volume 40, Issue 1, pp 275–284 | Cite as

Polysaccharides from Arnebia euchroma Ameliorated Endotoxic Fever and Acute Lung Injury in Rats Through Inhibiting Complement System

  • Ying-ye Ou
  • Yun Jiang
  • Hong Li
  • Yun-yi Zhang
  • Yan Lu
  • Dao-feng Chen


Arnebiaeuchroma (Royle) Johnst (Ruanzicao) is a traditional Chinese herbal medicine (TCM). It is extensively used in China and other countries for treatment of inflammatory diseases. It is known that hyper-activated complement system involves in the fever and acute lung injury (ALI) in rats. In our preliminary studies, anti-complementary activity of crude Arnebiaeuchroma polysaccharides (CAEP) had been demonstrated in vitro. This study aimed to investigate the role and mechanism of crude Arnebiaeuchroma polysaccharides (CAEP) using two animal models, which relate with inappropriate activation of complement system. In lipopolysaccharide (LPS)-induced fever model, the body temperature and leukocytes of peripheral blood in rats were significantly increased, while the complement levels of serum were remarkably decreased. CAEP administration alleviated the LPS-induced fever, reduced the number of leukocytes, and improved the levels of complement. Histological assay showed that there were severe damages and complement depositions in lung of the ALI rats. Further detection displayed that the oxidant stress was enhanced, and total hemolytic activity and C3/C4 levels in serum were decreased significantly in the ALI model group. Remarkably, CAEP not only attenuated the morphological injury, edema, and permeability in the lung but also significantly weakened the oxidant stress in bronchoalveolar lavage fluid (BALF) in the ALI rats. The levels of complement and complement depositions were improved by the CAEP treatment. In conclusion, the CAEP treatment ameliorated febrile response induced by LPS and acute lung injury induced by LPS plus ischemia-reperfusion. CAEP exerted beneficial effects on inflammatory disease potentially via inhibiting the inappropriate activation of complement system.


acute lung injury fever polysaccharides Arnebia euchroma complement 



This work was supported by grants from the Ministry of Science and Technology of China (2012ZX09301001-003), the National Natural Science Foundation of China (81330089, 30925042), the Committee of Science and Technology of Shanghai Municipality, China (10XD1405900, 08DZ1971202), and the Foundation for the Doctoral Program from the Ministry of Education of China (200802460055).

Compliance with Ethical Standards

The use of animals was approved by the Animal Ethical Committee of School of Pharmacy of Fudan University (Ethical Review No. 2011-9).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10753_2016_478_MOESM1_ESM.pdf (318 kb)
ESM 1 (PDF 317 kb)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Pharmacology, School of PharmacyFudan UniversityShanghaiChina
  2. 2.Department of Pharmacognosy, School of PharmacyFudan UniversityShanghaiChina

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