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Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells

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ABSTRACT

The aim of this study was to examine the anti-inflammatory effect of brain-derived neurotrophic factor (BDNF) on human dental pulp cells (HDPCs) and identify the intracellular signaling pathway involved. We investigated the effect of BDNF (50 ng/ml) on interleukin (IL)-6 and IL-8 expression in peptidoglycan (PGN)-treated HDPCs. An inhibition assay was performed with MAPK or NF-κB inhibitors to determine the possible signaling pathway. IL-6 and IL-8 mRNA, IL-6 and IL-8 protein, and phosphorylated p38 kinase activity were determined using real-time PCR, ELISA, and Western blot analysis, respectively. BDNF significantly attenuated PGN-induced IL-6 and IL-8 mRNA and protein levels in HDPCs. A p38 inhibitor also inhibited IL-6 and IL-8 mRNA transcription. PGN stimulated phosphorylated p38 kinase activity in HDPCs, which was inhibited by BDNF. Suppression of phosphorylated p38 kinase activity by BDNF in HDPCs inhibited increased IL-6 and IL-8 expression induced by PGN. Our findings suggest that BDNF regulates intracellular signaling molecule activities to exert its anti-inflammatory effect.

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ACKNOWLEDGMENTS

This work was supported in part by JSPS KAKENHI Grant Number JP25463218.

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Authors and Affiliations

  1. Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Katsuhiro Takeda, Naoko Tokunaga, Yusuke Aida, Mikihito Kajiya, Kazuhisa Ouhara, Shinya Sasaki, Noriyoshi Mizuno, Tsuyoshi Fujita & Hidemi Kurihara

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  1. Katsuhiro Takeda
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  2. Naoko Tokunaga
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  3. Yusuke Aida
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  4. Mikihito Kajiya
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  5. Kazuhisa Ouhara
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  6. Shinya Sasaki
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  7. Noriyoshi Mizuno
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  8. Tsuyoshi Fujita
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  9. Hidemi Kurihara
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Correspondence to Katsuhiro Takeda.

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Takeda, K., Tokunaga, N., Aida, Y. et al. Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells. Inflammation 40, 240–247 (2017). https://doi.org/10.1007/s10753-016-0474-4

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  • DOI: https://doi.org/10.1007/s10753-016-0474-4

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