, Volume 40, Issue 1, pp 240–247 | Cite as

Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells

  • Katsuhiro Takeda
  • Naoko Tokunaga
  • Yusuke Aida
  • Mikihito Kajiya
  • Kazuhisa Ouhara
  • Shinya Sasaki
  • Noriyoshi Mizuno
  • Tsuyoshi Fujita
  • Hidemi Kurihara


The aim of this study was to examine the anti-inflammatory effect of brain-derived neurotrophic factor (BDNF) on human dental pulp cells (HDPCs) and identify the intracellular signaling pathway involved. We investigated the effect of BDNF (50 ng/ml) on interleukin (IL)-6 and IL-8 expression in peptidoglycan (PGN)-treated HDPCs. An inhibition assay was performed with MAPK or NF-κB inhibitors to determine the possible signaling pathway. IL-6 and IL-8 mRNA, IL-6 and IL-8 protein, and phosphorylated p38 kinase activity were determined using real-time PCR, ELISA, and Western blot analysis, respectively. BDNF significantly attenuated PGN-induced IL-6 and IL-8 mRNA and protein levels in HDPCs. A p38 inhibitor also inhibited IL-6 and IL-8 mRNA transcription. PGN stimulated phosphorylated p38 kinase activity in HDPCs, which was inhibited by BDNF. Suppression of phosphorylated p38 kinase activity by BDNF in HDPCs inhibited increased IL-6 and IL-8 expression induced by PGN. Our findings suggest that BDNF regulates intracellular signaling molecule activities to exert its anti-inflammatory effect.


brain-derived neurotrophic factor peptidoglycan pulp cells endodontic treatment 



This work was supported in part by JSPS KAKENHI Grant Number JP25463218.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Katsuhiro Takeda
    • 1
  • Naoko Tokunaga
    • 1
  • Yusuke Aida
    • 1
  • Mikihito Kajiya
    • 1
  • Kazuhisa Ouhara
    • 1
  • Shinya Sasaki
    • 1
  • Noriyoshi Mizuno
    • 1
  • Tsuyoshi Fujita
    • 1
  • Hidemi Kurihara
    • 1
  1. 1.Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health SciencesHiroshima UniversityHiroshimaJapan

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