Skip to main content

Advertisement

SpringerLink
Log in

Effects of CCK-8 and Cystathionine γ-Lyase/Hydrogen Sulfide System on Acute Lung Injury in Rats

  • ORIGINAL ARTICLE
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Acute lung injury (ALI) is mainly characterized by diffusive injuries to lung epithelium and increased permeability of alveolar-capillary membranes caused by various factors, which leads to pulmonary edema and pulmonary closure. Lipopolysaccharide (LPS), which is the main component of the cell wall of gram-negative bacteria, is one of the most important factors causing pulmonary infection and ALI. More and more reports have indicated that hydrogen sulfide (H2S) is closely correlated with ALI and has anti-inflammation function, while the specific mechanism needs further investigation. Cholecystokinin-octapeptide (CCK-8), which is an important endogenous functional fragment belonging to CCK family, participates in anti-inflammatory and anti-endotoxic shock (ES). Whether CCK-8 plays important roles in curing ALI also needs further investigation. Herein, we concluded that CCK-8 alleviated the ALI induced by LPS via regulating the catalytic activity of cystathionine γ-lyase (CSE) and the formation of H2S. This work provides new medicine-designed target for clinical doctor to prevent and cure ALI.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Reference

  1. Mouratis, M.A., et al. 2015. Autotaxin and Endotoxin-Induced Acute Lung Injury. PLoS One 10(7): e0133619.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Ware, L.B. 2006. Pathophysiology of acute lung injury and the acute respiratory distress syndrome. Seminars in Respiratory and Critical Care Medicine 27(4): 337–49.

    Article  PubMed  Google Scholar 

  3. Donahoe, M. 2011. Acute respiratory distress syndrome: A clinical review. Pulmonary Circulatory 1(2): 192–211.

    Article  Google Scholar 

  4. Ragaller, M., and T. Richter. 2010. Acute lung injury and acute respiratory distress syndrome. Journal of Emergencies, Trauma and Shock 3(1): 43–51.

    Article  PubMed Central  Google Scholar 

  5. Elizabeth, R., and A.M.A.M. Johnson. 2010. Acute Lung Injury Epidemiology, Pathogenesis, and Treatment. Journal Aerosol Medicine Pulmonary Drug Delivery 23(4): 243–252.

    Article  Google Scholar 

  6. Brown, L., et al. 2015. Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews Microbiology 13(10): 620–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Ramachandran, G. 2014. Gram-positive and gram-negative bacterial toxins in sepsis: a brief review. Virulence 5(1): 213–8.

    Article  PubMed  Google Scholar 

  8. Rittirsch, D., et al. 2008. Acute Lung Injury Induced by Lipopolysaccharide Is Independent of Complement Activation. The Journal of Immunology 180(11): 7664–7672.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gu, H., et al. 2013. Necro-inflammatory response of pancreatic acinar cells in the pathogenesis of acute alcoholic pancreatitis. Cell Death & Disease 4, e816.

    Article  CAS  Google Scholar 

  10. Li, S., et al. 2007. CCK-8 inhibits LPS-induced IL-1beta production in pulmonary interstitial macrophages by modulating PKA, p38, and NF-kappaB pathway. Shock 27(6): 678–86.

    Article  CAS  PubMed  Google Scholar 

  11. Li, S., Z. Ni, B. Cong, W. Gao, S. Xu, C. Wang, Y. Yao, C. Ma, and Y. Ling. 2007. Cholecystokinin plays a novel protective role in diabetic kidney through anti-inflammatory actions on macrophage anti-inflammatory effect of cholecystokinin. Shock 27(6): 678–686.

    Article  CAS  PubMed  Google Scholar 

  12. Romero, C. and M.A. Bosch. Effect of dimethylthiourea in phosphatidylcholine biosynthesis by rat lung during reversible endotoxic shock. (0300–8177 (Print)).

  13. Huang, X.-L., Y.-L. Ling, Y.-Q. Ling, J.-L. Zhou, Y. Liu, and Q.-H. Wang. 2004. Heme oxygenase-1 in cholecystokinin-octapeptipe attenuated injury of pulmonary artery smooth muscle cells induced by lipopolysaccharide and its signal transduction mechanism. World Journal of Gastroenterol 10(12): 1789–1794.

    Article  CAS  Google Scholar 

  14. Ling, Y.-L., X.-L. Huang, Y.-Q. Ling, J.-L. Zhou, Y. Liu, and Q.-H. Wang. 2004. Heme oxygenase-1 in cholecystokinin-octapeptipe attenuated injury of pulmonary artery smooth muscle cells induced by lipopolysaccharide and its signal transduction mechanism. World Journal of Gastroenterology 10(12): 1789–1794.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Guo, H., et al. 2014. Electroacupuncture Ameliorates Acute Lung Injury through Promoting Gastrointestinal Motility in Rats with Acute Pancreatitis. Evidence-Based Complementary and Alternative Medicine 2014: 1–8.

    Google Scholar 

  16. Bhatia, H.Z.A.M. 2009. Role of Hydrogen Sulfide in Acute Lung Injury and Acute Respiratory Distress Syndrome. The Open Critical Care Medicine Journal 2: 13–17.

    Google Scholar 

  17. Fu, M., et al. 2012. Hydrogen sulfide (H2S) metabolism in mitochondria and its regulatory role in energy production. Proceedings of the National Academy of Sciences of the United States of America 109(8): 2943–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. You, X.J., et al. 2011. Expression of cystathionine beta-synthase and cystathionine gamma-lyase in human pregnant myometrium and their roles in the control of uterine contractility. PLoS One 6(8): e23788.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Patel, P., et al. 2009. The endogenous production of hydrogen sulphide in intrauterine tissues. Reproductive Biology and Endocrinology 7: 10.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Madden, J.A., et al. 2012. Precursors and inhibitors of hydrogen sulfide synthesis affect acute hypoxic pulmonary vasoconstriction in the intact lung. Journal of Applied Physiologica (1985) 112(3): 411–8.

    Article  CAS  Google Scholar 

  21. Li, T., et al. 2008. Regulatory effects of hydrogen sulfide on IL-6, IL-8 and IL-10 levels in the plasma and pulmonary tissue of rats with acute lung injury. Experimental Biology and Medicine (Maywood, NJ) 233(9): 1081–7.

    Article  CAS  Google Scholar 

  22. Faller, S., K. Zimmermann, K.M. Strosing, H. Engelstaedter, H. Buerkle, R. Schmidt, S.G. Spassov, and A. Hoetzel. 2012. Inhaled hydrogen sulfide protects against lipopolysaccharide-induced acute lung injury in mice. Medicine Gas Research 12(26): 1–6.

    Google Scholar 

  23. Runmin Guo, K.W., J. Chen, L. Mo, X. Hua, D. Zheng, P. Chen, G. Chen, W. Xu, and J. Feng. 2013. Exogenous Hydrogen Sulfide Protects against Doxorubicin-Induced Inflammation and Cytotoxicity by Inhibiting p38MAPK/NFκB Pathway in H9c2 Cardiac Cells. Cellular Physiology and Biochemistry 32: 1668–1680.

    PubMed  Google Scholar 

  24. Zhou, X.H., X. Huang, P. Wei, F.J. Tian, and Y.L. Ling. 2009. Role of hydrogen sulfidecystathionine-gamma-lyase system in acute lung injury induced by lipopolysaccharide in rats. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 21(4): 199–202.

    CAS  PubMed  Google Scholar 

  25. Smith, K.M., et al., Prolonged partial liquid ventilation using conventional and high-frequency ventilatory techniques: gas exchange and lung pathology in an animal model of respiratory distress syndrome. (0090–3493 (Print)).

  26. Szapiel, S.V., N. Elson, J.D. Fulmer, and G.W. Hunninghake. 1979. Crystal RG, Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse. The American Review of Respiratory Disease 120(4): 893–899.

  27. Chen, X., et al. 2014. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-kappaB expression, decreasing TNF-alpha levels and increasing IL-10 levels. Experimental and Therapeutic Medicine 8(2): 464–470.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Huang, S., H. Li, and J. Ge. 2015. A cardioprotective insight of the cystathionine γ-lyase/hydrogen sulfide pathway. IJC Heart & Vasculature 7: 51–57.

    Article  Google Scholar 

  29. Pan, L.L., et al. 2012. Role of cystathionine gamma-lyase/hydrogen sulfide pathway in cardiovascular disease: a novel therapeutic strategy? Antioxidants and Redox Signaling 17(1): 106–18.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Chiku, T., et al. 2009. H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia. Journal of Biological Chemistry 284(17): 11601–12.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Leffler, C.W., et al. 2006. Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation. Journal of Applied Physiologica (1985) 100(3): 1065–76.

    Article  CAS  Google Scholar 

  32. Ling, Y.-L., A.-H. Meng, X.-P. Zhang, and J.-L. Zhang. 2002. Anti-inflammatory effect of cholecystokinin and its signal transduction mechanism in endotoxic shock rat. World Journal of Gastroenterology 8(4): 712–717.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Verspohl Ej Fau - Wunderle, G., et al., Proglumide (gastrin and cholecystokinin receptor antagonist) inhibits insulin secretion in vitro. (0028–1298 (Print)).

Download references

Author information

Authors and Affiliations

  1. No. 2 Department of Respiratory, The Third Hospital of Hebei Medical University, Hebei, People’s Republic of China

    Fengjun Tian & Yuqin Chen

  2. Department of Pathophysiology, Hebei Medical University, Hebei, People’s Republic of China

    Yiling Ling

  3. Department of Critical Care Medicine, The Third Hospital of Hebei Medical University, No. 139 Ziqiang Road, Qiaoxi District, Shijazhuang, Hebei, 050051, China

    Zhiyong Wang

Authors
  1. Fengjun Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yiling Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuqin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiyong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhiyong Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tian, F., Ling, Y., Chen, Y. et al. Effects of CCK-8 and Cystathionine γ-Lyase/Hydrogen Sulfide System on Acute Lung Injury in Rats. Inflammation 40, 174–183 (2017). https://doi.org/10.1007/s10753-016-0466-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-016-0466-4

KEY WORDS

Search

Navigation