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Inflammation

, Volume 39, Issue 3, pp 1198–1204 | Cite as

Genetic Polymorphisms of X-ray Repair Cross-Complementing Group 1 and Apurinic/Apyrimidinic Endonuclease-1 in Chronic Obstructive Pulmonary Disease

  • Avinash Bardia
  • Sandeep Kumar Vishwakarma
  • Chandrakala Lakki Reddy
  • N. Raju
  • Shaik Iqbal
  • Gallapalli Sravani
  • Narneni Lavanya
  • Nazima Begum
  • Naziya Usma
  • Pratibha Nallari
  • Baderuzzaman
  • Syed Mehmood Ahmed
  • Asfaq Hasan
  • Aleem A. Khan
ORIGINAL ARTICLE
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Abstract

Chronic obstructive pulmonary disease (COPD) is a heterogeneous collection of conditions characterized by irreversible expiratory airflow limitation. The disease is interspersed with exacerbations; periods of acute symptomatic, physiological, and functional deterioration. The present study was designed to investigate the role of X-ray cross-complementing group 1 (XRCC1) and apurinic/apyrimidinic endonuclease 1 (APE1) polymorphisms and the risk of COPD. Blood samples from 354 unrelated subject (age range 18–60 years; 156 with COPD, 198 healthy controls) were collected. Genomic DNA was isolated and genotyped for XRCC1 Arg399Gln and APE1 Asp148Glu using a confronting two pair primers polymerase chain reaction. GA genotype of XRCC1 gene was found to be predominant in the COPD group compared to controls with 1.86-fold increased risk for COPD (OR 1.86, 95 % CI 1.20–2.88, p = 0.0013). TG genotype of APE1 was found to be predominant in COPD group compared to controls with the difference being statistically significant (OR 1.68, 95 % CI 1.08–2.61, p = 0.0043). The GA haplotype was found to be predominant in COPD than controls with a 2.19-fold significant increase (OR 2.19, 95 % CI 1.46–3.28, p = 0.003). Polymorphism in XRCC1 and APE1 gene is associated with an increased risk of COPD.

KEY WORDS

COPD APE1 XRCC1 polymorphisms 

Notes

Acknowledgments

The study was not supported by any intramural or extramural funds.

Compliance with ethical standards

Conflict of interest

The authors hereby declare no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Avinash Bardia
    • 1
  • Sandeep Kumar Vishwakarma
    • 1
  • Chandrakala Lakki Reddy
    • 1
  • N. Raju
    • 1
  • Shaik Iqbal
    • 1
  • Gallapalli Sravani
    • 1
  • Narneni Lavanya
    • 1
  • Nazima Begum
    • 1
  • Naziya Usma
    • 2
  • Pratibha Nallari
    • 3
  • Baderuzzaman
    • 4
  • Syed Mehmood Ahmed
    • 5
  • Asfaq Hasan
    • 5
  • Aleem A. Khan
    • 1
  1. 1.Central Laboratory for Stem Cell Research & Translational Medicine, Centre for Liver Research and DiagnosticsDeccan College of Medical SciencesHyderabadIndia
  2. 2.Department of PhysiologyDeccan College of Medical SciencesHyderabadIndia
  3. 3.Department of GeneticsOsmania UniversityHyderabadIndia
  4. 4.Department of BiochemistryDeccan College of Medical SciencesHyderabadIndia
  5. 5.Department of Pulmonology, Owaisi Hospital & Research CentreDeccan College of Medical SciencesHyderabadIndia

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