Protective Effects of Lactobacillus plantarum NDC 75017 Against Lipopolysaccharide-Induced Liver Injury in Mice
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This study investigated the protective effect of Lactobacillus plantarum NDC 75017 (L. plantarum NDC 75017) against acute liver injury induced by lipopolysaccharide (LPS). Thirty male mice were randomly divided into the control, LPS, and LPS + L. plantarum NDC 75017 groups. In the LPS + L. plantarum group, the mice were orally pretreated with L. plantarum NDC 75017 for 15 days. At 16 days, the mice in the LPS and LPS + L. plantarum NDC 75017 groups were intraperitoneally injected with LPS at 4 mg/kg body weight, whereas the control mice were treated with an equal amount of saline. After 8 h, the serum alanine transaminase (ALT), aspartate aminotransferase (AST), and histology changes were examined. The oxidative stress markers and pro-inflammatory cytokines in the liver were also examined. Meanwhile, the expression of nuclear factor κB (NF-κB) mRNA and toll-like receptor 4 (TLR4) in the liver was determined by qRT-PCR. The LPS group showed an increase in ALT and AST, whereas the LPS + L. plantarum NDC 75017 group showed a significant decrease. In addition, pretreatment with L. plantarum NDC 75017 can attenuate LPS-induced oxidative stress and inflammatory response. Furthermore, the increase of hepatic NF-κB and TLR4 mRNA induced by LPS was significantly downregulated by the pretreatment with L. plantarum NDC 75017. These data show that pretreatment with L. plantarum NDC 75017 protects against LPS-induced oxidative stress and inflammatory injury in the liver of mice, which may be attributed to the inhibition of the TLR4-NF-κB pathway.
KEY WORDSLactobacillus plantarum NDC 75017 liver toll-like receptor 4 nuclear factor κB mice
This study was supported by the National Science and Technology Project (grant number 2011AA100902), the Science and Technology Project for the Universities of Shandong Province (grant number J13LE55), the National Natural Science Foundation of China (grant number 31171718), and the China Postdoctoral Science Foundation (grant number 2012 M510911).
Conflict of Interest
The authors declare that there are no conflicts of interest.
- 1.Laubach, H.J., P.M. Jakob, K.O. Loevblad, A.E. Baird, M.P. Bovo, R.R. Edelman, and S. Warach. 1998. A phantom for diffusion-weighted imaging of acute stroke. Journal of Magnetic Resonance Imaging 8: 1349–1354.Google Scholar
- 10.Seimon, T.A., A. Obstfeld, K.J. Moore, D.T. Golenbock, and I. Tabas. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proceedings of the National Academy of Sciences of the United States of America 103: 19794–19799.PubMedCrossRefPubMedCentralGoogle Scholar
- 11.Ben Ari, Z., O. Avlas, O. Pappo, V. Zilbermints, Y. Cheporko, L. Bachmetov, R. Zemel, A. Shainberg, E. Sharon, F. Grief, and E. Hochhauser. 2012. Reduced hepatic injury in Toll-like receptor 4-deficient mice following D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure. Cellular Physiology and Biochemistry 29: 41–50.PubMedCrossRefGoogle Scholar
- 12.Wang, Y., Y. Li, J. Xie, Y. Zhang, J. Wang, X. Sun, and H. Zhang. 2013. Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and d-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities. International Immunopharmacology 15: 30–37.PubMedCrossRefGoogle Scholar
- 14.Wang, Y., Y. Liu, A. Sidhu, Z. Ma, C. McClain, and W. Feng. 2012. Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury. American Journal of Physiology - Gastrointestinal and Liver Physiology 303: G32–G41.PubMedCrossRefPubMedCentralGoogle Scholar
- 23.Nardone, G., D. Compare, E. Liguori, V. Di Mauro, A. Rocco, M. Barone, A. Napoli, D. Lapi, M.R. Iovene, and A. Colantuoni. 2010. Protective effects of Lactobacillus paracasei F19 in a rat model of oxidative and metabolic hepatic injury. American Journal of Physiology - Gastrointestinal and Liver Physiology 299: G669–G676.PubMedCrossRefGoogle Scholar
- 24.Kaur, G., N. Tirkey, S. Bharrhan, V. Chanana, P. Rishi, and K. Chopra. 2006. Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents. Clinical and Experimental Immunology 145: 313–321.PubMedCrossRefPubMedCentralGoogle Scholar
- 29.Deguchi, R., H. Nakaminami, E. Rimbara, N. Noguchi, M. Sasatsu, T. Suzuki, M. Matsushima, J. Koike, M. Igarashi, H. Ozawa, R. Fukuda, and A. Takagi. 2012. Effect of pretreatment with Lactobacillus gasseri OLL2716 on first-line Helicobacter pylori eradication therapy. Journal of Gastroenterology and Hepatology 27: 888–892.PubMedCrossRefPubMedCentralGoogle Scholar
- 30.Giriwono, P.E., H. Shirakawa, H. Hokazono, T. Goto, and M. Komai. 2011. Fermented barley extract supplementation maintained antioxidative defense suppressing lipopolysaccharide-induced inflammatory liver injury in rats. Bioscience, Biotechnology, and Biochemistry 75: 1971–1976.PubMedCrossRefGoogle Scholar
- 32.Mendis, E., M.M. Kim, N. Rajapakse, and S.K. Kim. 2008. Suppression of cytokine production in lipopolysaccharide-stimulated mouse macrophages by novel cationic glucosamine derivative involves down-regulation of NF-kappaB and MAPK expressions. Bioorganic and Medicinal Chemistry 16: 8390–8396.PubMedCrossRefGoogle Scholar
- 35.Herath, T.D., R.P. Darveau, C.J. Seneviratne, C.Y. Wang, Y. Wang, and L. Jin. 2013. Tetra- and penta-acylated lipid A structures of Porphyromonas gingivalis LPS differentially activate TLR4-mediated NF-kappaB signal transduction cascade and immuno-inflammatory response in human gingival fibroblasts. PLoS One 8: e58496.PubMedCrossRefPubMedCentralGoogle Scholar
- 36.Joo, H.M., Y.J. Hyun, K.S. Myoung, Y.T. Ahn, J.H. Lee, C.S. Huh, M.J. Han, and D.H. Kim. 2011. Lactobacillus johnsonii HY7042 ameliorates Gardnerella vaginalis-induced vaginosis by killing Gardnerella vaginalis and inhibiting NF-kappaB activation. International Immunopharmacology 11: 1758–1765.PubMedCrossRefGoogle Scholar
- 37.Shimazu, T., J. Villena, M. Tohno, H. Fujie, S. Hosoya, T. Shimosato, H. Aso, Y. Suda, Y. Kawai, T. Saito, S. Makino, S. Ikegami, H. Itoh, and H. Kitazawa. 2012. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infection and Immunity 80: 276–288.PubMedCrossRefPubMedCentralGoogle Scholar