, Volume 37, Issue 5, pp 1424–1431 | Cite as

Inhibitory Effects of Rutin on the Endothelial Protein C Receptor Shedding In Vitro and In Vivo

  • Sae-Kwang Ku
  • In-Chul Lee
  • Min-Su Han
  • Jong-Sup Bae


Endothelial cell protein C receptor (EPCR) has important functions in regulation of coagulation and inflammation. EPCR shedding from the cell surface is mediated by tumor necrosis factor-α converting enzyme (TACE). Rutin is one of the major flavonoids from the buckwheat plant Fagopyrum tataricum. In this study, we investigated the effects of rutin on phorbol-12-myristate 13-acetate (PMA), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and on cecal ligation and puncture (CLP)-mediated EPCR shedding. We used a CLP model because this model more closely resembles human sepsis. Data showed rutin was a potent inhibitor of PMA, TNF-α, IL-1β, and CLP-induced EPCR shedding by suppression of TACE expression. Treatment with rutin resulted in a decrease of PMA-stimulated phosphorylation of p38, extracellular regulated kinases 1/2, and c-Jun N-terminal kinase. These results suggest the potential application of rutin for treatment of PMA and CLP-mediated EPCR shedding.


rutin EPCR shedding PMA CLP 



This study was supported by the National Research Foundation of Korea (NRF) funded by the Korea government [MSIP] (Grant no. 2013–067053).

Conflict of Interest



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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sae-Kwang Ku
    • 1
  • In-Chul Lee
    • 2
  • Min-Su Han
    • 3
  • Jong-Sup Bae
    • 4
  1. 1.Department of Anatomy and Histology, College of Korean MedicineDaegu Haany UniversityGyeongsanRepublic of Korea
  2. 2.Department of Cosmetic Science and TechnologySeowon UniversityCheongjuRepublic of Korea
  3. 3.Laboratory for Arthritis and Bone BiologyFatima Research Institute, Daegu Fatima HospitalDaeguRepublic of Korea
  4. 4.College of Pharmacy, CMRI, Research Institute of Pharmaceutical SciencesKyungpook National UniversityBuk-guRepublic of Korea

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