, Volume 38, Issue 3, pp 1080–1084 | Cite as

Preventive Effects of Protocatechuic Acid on LPS-Induced Inflammatory Response in Human Gingival Fibroblasts via Activating PPAR-γ

  • Yimin Wang
  • Jun Zhou
  • Sheng Fu
  • Cheng Wang
  • Bing Zhou


Protocatechuic acid (PCA), a major metabolite of anthocyanins, has been shown to have antioxidant, antitumoral, and anti-inflammatory activities. In this study, we investigated the anti-inflammatory effects of PCA on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). The effects of PCA on LPS-induced inflammatory cytokines IL-6 and chemokines IL-8 in HGFs were detected by ELISA. The expression of NF-κB and PPAR-γ was detected by Western blot analysis. Our results demonstrated that PCA suppressed IL-6 and IL-8 production in LPS-stimulated HGFs. We also found that PCA inhibited LPS-induced NF-κB activation. Furthermore, the inhibition of PCA on LPS-induced IL-6 and IL-8 production can be reversed by PPAR-γ antagonist GW9662. In conclusion, the anti-inflammatory mechanism of PCA is associated with activating PPAR-γ, thereby inhibiting LPS-induced inflammatory response.


protocatechuic acid human gingival fibroblasts cytokine NF-κB PPAR-γ 


Conflict of Interest

All authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yimin Wang
    • 1
  • Jun Zhou
    • 2
  • Sheng Fu
    • 1
  • Cheng Wang
    • 1
  • Bing Zhou
    • 1
  1. 1.Department of StomatologyFuzhou General Hospital of Nanjing Military CommandFuzhouPeople’s Republic of China
  2. 2.Medical DepartmentFuzhou General Hospital of Nanjing Military CommandFuzhouPeople’s Republic of China

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