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Pharmacokinetic and Anti-inflammatory Effects of Sanguinarine Solid Lipid Nanoparticles

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Abstract

The sanguinarine (SG) was studied for its pharmacokinetic and anti-inflammatory activities with prepared solid lipid nanoparticles (SLNs). The sanguinarine solid lipid nanoparticles (SG-SLNs) were prepared by film-ultrasonic dispersion method and the entrapment efficiency of SG was higher at 75.6 %. The drug release profile of SG was examined in pH 7.4 PBS and 85 % of the SG loaded in SLNs was gradually released during 24 h. We used mice endotoxin shock model which was induced by lipopolysaccharide (1 mg/kg) to examine the anti-inflammatory function of SG-SLNs. Healthy Kunming mice were administered orally with saline, SG (10 mg/kg), and SG-SLNs (10 mg/kg), respectively, at 12 and 1 h before lipopolysaccharide (LPS) injection. Mice were sacrificed at 1 and 6 h, respectively, and blood was collect through the venous sinus to access inflammatory mediators. Pharmacokinetic studies proved that the AUC0→24 and C max of SG-SLNs were significantly increased compared that of SG. SG-SLNs revealed significant anti-inflammatory effects through inhibition of LPS-induced tumor necrosis factor-alpha level, interleukin 6 level, and nitric oxide production in serum. Therefore, it can be concluded that SG-SLNs led to a better oral bioavailability.

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Acknowledgments

This work was supported by a research grant from Xi’an Jiaotong University.

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Authors and Affiliations

  1. School of Pharmacy, Xi’an Jiaotong University, No. 76 Western Yanta Road, Xi’an City, Shaanxi Province, 710061, People’s Republic of China

    Weifeng Li, Huani Li, Huan Yao, Qingli Mu, Guilan Zhao, Yongmei Li, Hua Hu & Xiaofeng Niu

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  1. Weifeng Li
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  2. Huani Li
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  3. Huan Yao
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  4. Qingli Mu
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  6. Yongmei Li
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  7. Hua Hu
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  8. Xiaofeng Niu
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Correspondence to Xiaofeng Niu.

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Li, W., Li, H., Yao, H. et al. Pharmacokinetic and Anti-inflammatory Effects of Sanguinarine Solid Lipid Nanoparticles. Inflammation 37, 632–638 (2014). https://doi.org/10.1007/s10753-013-9779-8

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