Skip to main content
SpringerLink
Log in

Comparison of α2-Macroglobulin Synthesis by Juvenile vs. Mature Rats after Identical Inflammatory Stimulation

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Synthesis of α2-macoglobulin (α2M) by 3-week-old juvenile rats was compared to that of mature 7- and 11-week-old rats. Serum concentrations of α2M, interleukin (IL)-6- and cytokine-induced neutrophil chemoattractant (CINC)-1 were measured by enzyme-linked immunosorbent assay. The area under the concentration vs. time curve (AUC) for α2M was significantly different among the three groups. The synthesis of α2M increased in an age-dependent manner. No significant difference was observed for the AUC of IL-6, but that of CINC-1 in 3-week-old rats was significantly lower than that in 7- or 11-week-old rats. These results suggest that synthesis of α2M was increased in mature compared to juvenile rats, possibly due to differences in liver function. The maximum concentration of CINC-1 in 3-week-old rats was observed 6 h after turpentine oil injection. The serum concentrations of IL-6 and CINC-1 increased more quickly in juvenile rats than in mature rats after inflammatory stimulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Capsi, D., M.L. Baltz, F. Snel, E. Gruys, D. Niv, R.M. Batt, et al. 1984. Isolation and characterization of C-reactive protein from the dog. Immunology 53: 307–313.

    Google Scholar 

  2. Ceron, J.J., P.D. Eckersall, and S. Martinez-Subiela. 2005. Acute phase in dogs and cats: current knowledge and future perspectives. Veterinaria Clinicology Pathology 34: 85–99.

    Article  Google Scholar 

  3. T.W., Du-Clos. 2000. Function of C-reactive protein. Animal Medicine 32: 274–278.

    Google Scholar 

  4. Eckersall, P.D., S. Duthie, M. Toussaint, E. Gruys, P. Heegaars, M.A.C. Lipperheide, et al. 1994. Standardization of diagnostic assays for animal acute phase proteins. Advances in Veterinary Medicine 41: 643–655.

    Article  Google Scholar 

  5. Eckersall, P.D., M.J.A. Harvey, J.M. Ferguson, J.P. Renton, D.A. Nickson, and J.S. Boyd. 1993. Acute phase proteins in canine pregnancy (Canis familiaris). Journl of Reproductive Fertility 47: 159–164.

    CAS  Google Scholar 

  6. Inoue, S., T. Jinbo, M. Shino, K. Iguchi, M. Nomura, K. Kawato, et al. 2001. Determination of α2-macroglobulin concentrations in healthy rats of various ages and rats inoculated with turpentine oil by enzyme-linked immunosorbent assay. Journal of Experimental Animal Science 42: 44–49.

    Article  Google Scholar 

  7. Honjo, T., T. Kuribayashi, M. Matsumoto, S. Yamazaki, and S. Yamamoto. 2010. Kinetics of α2-macrogobulin and α1-acid glycoprotein in rats subjected to repeated acute inflammatory stimulation. Laboratory Animals 44: 150–154.

    Article  PubMed  CAS  Google Scholar 

  8. Fisher, N.A., H.E. Poulsen, B.A. Hansen, N. Tygstrup, and S. Keiding. 1989. Age dependence of rat liver function measurements. Journal of Hepatology 9: 190–197.

    Article  Google Scholar 

  9. Jinbo, T., M. Motoki, and S. Yamamoto. 2001. Variation of serum α2-macroglobulin concentration in healthy rats and rats inoculated Staphylococcus aureus or subjected to surgery. Comparative Medicine 51: 332–335.

    PubMed  CAS  Google Scholar 

  10. Kuribayashi, T., M. Tomizawa, T. Seita, K. Tagata, and S. Yamamoto. 2011. Relationship between production of acute-phase proteins and strength of inflammatory stimulation in rats. Laboratory Animals 45: 215–218.

    Article  PubMed  CAS  Google Scholar 

  11. Kuribayashi, T., T. Shimada, M. Matsumoto, K. Kawato, T. Honjo, M. Fukuyama, et al. 2003. Determination of serum C-reactive protein (CRP) in healthy beagle dogs of various ages and pregnant beagle dogs. Experimental Animals 52: 387–390.

    Article  PubMed  CAS  Google Scholar 

  12. Karadag, F., S. Kirdar, A.B. Karul, and E. Ceylan. 2008. The value of C-reactive protein as a marker of systemic inflammation in stable chronic obstructive pulmonary disease. European Journal of Internal Medicine 19: 104–108.

    Article  PubMed  CAS  Google Scholar 

  13. Hayashi, S., T. Jinbo, K. Iguchi, M. Shimizu, T. Shimada, M. Nomura, et al. 2001. A comparison of the concentrations of C-reactive protein and alpha1-acid glycoprotein in the serum of young and adult dogs with acute inflammation. Veterinary Research Communications 25: 117–126.

    Article  PubMed  CAS  Google Scholar 

  14. Honjo, T., T. Kuribayashi, Y. Mokonuma, A. Yamaga, S. Yamazaki, and S. Yamamoto. 2010. The effects of interleukin-6 and cytokine-induced neutrophil chemoattractant-1 on α2-macroglobulin production in rat. Experimental Animals 59: 589–594.

    Article  PubMed  CAS  Google Scholar 

  15. Jinbo, T., T. Sakamoto, and S. Yamamoto. 2002. Serum α2-macroglobulin and cytokine measurements in an cute inflammation model in rats. Laboratory Animals 36: 153–157.

    Article  PubMed  CAS  Google Scholar 

  16. Charles River Laboratories Japan Inc., Research and development data, body weight curve, CD(SD), Available at: http://www.crj.co.jp, Accessed May 23, 2013.

  17. Okaichi, Y., and H. Okaichi. 1995. Development of spatial task performance in the radial arm maze in weaning rats. Japan Journal Animal Pyschology 45: 1–12. Japanese with English abstract.

    Article  Google Scholar 

  18. Gao, P., M.E. Guyton, T. Huang, J.M. Bauer, K.J. Stefanski, and Q. Lu. 2004. Enhanced oral bioavailability of a poorly water soluble drug PNU-91325 by supersaturatable formulations. Drug Development and Industrial Pharmacy 30: 221–229.

    Article  PubMed  CAS  Google Scholar 

  19. Atef, E., and A.A. Belmonte. 2008. Formulation and in vitro and in vivo characterization of phenytoin self-emulsifying drug delivery system (SEDDS). European Journal of Pharmaceutical Sciences 35: 257–263.

    Article  PubMed  CAS  Google Scholar 

  20. Horn, F., U.M. Wegenka, C. Lutticken, J. Yuan, E. Roeb, W. Boers, et al. 1982. Regulation of α2-macroglobulin gene expression by interleukin-6. Animals N Y Academic Science 737: 308–322.

    Article  Google Scholar 

  21. Ling, P.P., R.J. Smith, S. Kie, P. Boyce, and B.R. Bistrian. 2004. Effects of protein malnutrition on IL-6-mediated signaling in the liver and the systemic acute-phase response in rats. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 287: 801–808.

    Article  Google Scholar 

  22. Nijsten, M.W.N., E.R. DeGroot, H.J. TenDuis, J.H. Klaren, C.E. Hack, and L.A. Arden. 1987. Serum levels of interleukin-6 and acute phase response. Lancet 330: 921.

    Article  Google Scholar 

  23. Sheikh, N., K. Tron, J. Dudas, and G. Ramadori. 2006. Cytokine induced neutrophil chmoattractant-1 is released by the noninjured liver in a rat acute phase model. Laboratory Investigation 86: 800–814.

    PubMed  CAS  Google Scholar 

  24. Kuribayashi, T.,Seita, T., Momotani, E., Honjo, T., Yamazaki, S., Yamamoto, S. Correlation between synthesis of α2-macroglobulin in hepatocytes and changes in serum cytokine levels in rats after inflammatory stimulation. Scand. J. Lab. Anim. Sci.(in press).

  25. Van Bezooijen, C.F.A., C.F. Grell, and D.L. Knook. 1976. Albumin by liver parenchymal cells from young, adult and old rats. Biochemical and Biophysical Research Communications 71: 513–519.

    Article  PubMed  Google Scholar 

  26. Schmucker, D.L. 1998. Aging and the liver: an update. Journal of Gerontology 53A: B315–B320.

    CAS  Google Scholar 

  27. Pieri, C., I.Z.S. Nagy, G. Mazzufferi, and C. Giuli. 1975. The aging of rat liver as revealed by electron microscopic morphometry—I. Basic parameters. Experimental Gerontology 10: 291–304.

    Article  PubMed  CAS  Google Scholar 

  28. Pieri, C., I. Zs-Nagy, and G. Mazzufferi. 1975. The aging of rat liver as revealed by electron microscopic morphometry—II. Parameters of regenerated old liver. Experimental Gerontology 10: 341–349.

    Article  PubMed  CAS  Google Scholar 

  29. Monsoni, L., P.P. Mirand, M.L. Houlier, and M. Arnal. 1993. Age-related changes in protein synthesis measured in vivo in rat liver and gastrocnemius muscle. Mechanics Aging Development 68: 209–220.

    Article  Google Scholar 

  30. Serushago, B., C. Macdonald, S.H.S. Lee, A. Stadnyk, and R. Bortolussi. 1995. Interferon-gamma detection in cultures of newborn cells exposed to Listera monocytogenes. Journal of Interferon and Cytokine Research 15: 633–635.

    Article  PubMed  CAS  Google Scholar 

  31. Saito, M., N. Takahashi, S. Ueda, Y. Kuwabara, M. Komiyama, Y. Koike, et al. 2010. Cytokine profile in a premature infant with systemic Bacillus cereus infection. Paediatrics International 52: e34–e36.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was partially supported by a research project grant awarded by Azabu University.

Author information

Authors and Affiliations

  1. Graduate School of Environmental Health, Azabu University, 1-17-71 Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan

    Takashi Kuribayashi, Tetsuro Seita & Shizuo Yamamoto

  2. Laboratory of Immunology, Azabu University, 1-17-71 Chuo-ku, Fuchinobe, Sagamihara, Kanagawa, 252-5201, Japan

    Katsuhito Kawato

  3. Laboratory of Physiology, Department of Food and Nutrition, Kamakura Women’s University, 6-1-3 Ofuna, Kamakura, Kanagawa, 247-8512, Japan

    Shunsuke Yamazaki

Authors
  1. Takashi Kuribayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuro Seita
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Katsuhito Kawato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shunsuke Yamazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shizuo Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shizuo Yamamoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuribayashi, T., Seita, T., Kawato, K. et al. Comparison of α2-Macroglobulin Synthesis by Juvenile vs. Mature Rats after Identical Inflammatory Stimulation. Inflammation 36, 1448–1452 (2013). https://doi.org/10.1007/s10753-013-9685-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-013-9685-0

KEY WORDS

Search

Navigation