, Volume 36, Issue 6, pp 1448–1452 | Cite as

Comparison of α2-Macroglobulin Synthesis by Juvenile vs. Mature Rats after Identical Inflammatory Stimulation

  • Takashi Kuribayashi
  • Tetsuro Seita
  • Katsuhito Kawato
  • Shunsuke Yamazaki
  • Shizuo Yamamoto


Synthesis of α2-macoglobulin (α2M) by 3-week-old juvenile rats was compared to that of mature 7- and 11-week-old rats. Serum concentrations of α2M, interleukin (IL)-6- and cytokine-induced neutrophil chemoattractant (CINC)-1 were measured by enzyme-linked immunosorbent assay. The area under the concentration vs. time curve (AUC) for α2M was significantly different among the three groups. The synthesis of α2M increased in an age-dependent manner. No significant difference was observed for the AUC of IL-6, but that of CINC-1 in 3-week-old rats was significantly lower than that in 7- or 11-week-old rats. These results suggest that synthesis of α2M was increased in mature compared to juvenile rats, possibly due to differences in liver function. The maximum concentration of CINC-1 in 3-week-old rats was observed 6 h after turpentine oil injection. The serum concentrations of IL-6 and CINC-1 increased more quickly in juvenile rats than in mature rats after inflammatory stimulation.


juvenile rats mature rats α2M IL-6 CINC-1 



This research was partially supported by a research project grant awarded by Azabu University.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Takashi Kuribayashi
    • 1
  • Tetsuro Seita
    • 1
  • Katsuhito Kawato
    • 2
  • Shunsuke Yamazaki
    • 3
  • Shizuo Yamamoto
    • 1
  1. 1.Graduate School of Environmental HealthAzabu UniversitySagamiharaJapan
  2. 2.Laboratory of ImmunologyAzabu UniversitySagamiharaJapan
  3. 3.Laboratory of Physiology, Department of Food and NutritionKamakura Women’s UniversityKamakuraJapan

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