Continuous Renal Replacement Therapy (CRRT) Attenuates Myocardial Inflammation and Mitochondrial Injury Induced by Venovenous Extracorporeal Membrane Oxygenation (VV ECMO) in a Healthy Piglet Model
In this study, we investigated the myocardial inflammation and mitochondrial function during venovenous extracorporeal membrane oxygenation (VV ECMO) and further evaluated the effects of continuous renal replacement therapy (CRRT) on them. Eighteen piglets were assigned to the control group, ECMO group, and ECMO+CRRT group. Myocardial inflammation was assessed by the activity of myeloperoxidase (MPO), myocardial concentrations, and mRNA expression of TNF-α, IL-1β, and IL-6; mitochondrial function was assessed by activities of mitochondrial complexes I–V. VV ECMO elicited a general activation of serum and myocardial inflammation and significantly decreased the activities of mitochondrial complexes I and IV. After being combined with CRRT, serum and myocardial concentrations of IL-1β and IL-6, myocardial mRNA expression of IL-6, and the activity of MPO were decreased significantly; the activities of mitochondrial complexes were increased. We conclude that myocardial inflammation was activated during ECMO therapy, inducing mitochondrial injury; moreover, CRRT reduced myocardial inflammation and partially ameliorated mitochondrial function.
KEY WORDSvenovenous extracorporeal membrane oxygenation (VV ECMO) myocardial inflammation mitochondrial function continuous renal replacement therapy (CRRT)
This study was supported by 12th five-year Major Program of Army Grants (no. AWS11J03; no. AWS12J001); Jiangsu Province’s Special Project of Science and Technology in Medicine (BL2012006); and Jiangsu Province’s Key Medical Talent Program (RC2011128). We thank Prof. Dehua Gong, Prof. Daxi Ji, Prof. Zhihong Liu, and other physicians, perfusionists, and nurses of Research Institute of Nephrology in Jinling Hospital for helping us perform hemofiltration.
Conflict of Interest
The authors declare that they have no conflict of interest.
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