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Inflammation

, Volume 35, Issue 6, pp 1814–1824 | Cite as

Effect of Chelerythrine Against Endotoxic Shock in Mice and Its Modulation of Inflammatory Mediators in Peritoneal Macrophages Through the Modulation of Mitogen-Activated Protein Kinase (MAPK) Pathway

  • Weifeng Li
  • Ting Fan
  • Yanmin  Zhang
  • Xiaofeng Niu
  • Wei Xing
Article

Abstract

A quaternary benzo [c] alkaloid chelerythrine (CHE), which is a traditional herbal prescription, has been used for the treatment of various inflammatory diseases. To gain insight into the anti-inflammatory effect and molecular mechanisms underlying the anti-inflammatory activity of CHE, we used experimentally induced mice endotoxic shock moled and lipopolysaccharide (LPS)-induced murine peritoneal macrophages to examine the anti-inflammatory function of CHE. CHE displayed significant anti-inflammatory effects in experimentally induced mice endotoxic shock model in vivo through inhibition of LPS-induced tumor necrosis factor-alpha (TNF-α) level and nitric oxide (NO) production in serum. Additionally, our data suggest that CHE treatment inhibits LPS-induced TNF-α level and NO production in LPS-induced murine peritoneal macrophages through selective inhibition of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) activation. Moreover, the effects of CHE on NO and cytokine TNF-α production can possibly be explained by the role of p38 MAPK and ERK1/2 in the regulation of inflammatory mediators expression.

KEY WORDS

chelerythrine anti-inflammatory effect nitric oxide TNF-α mitogen-activated protein kinase 

Notes

Acknowledgments

This work was supported by a research grant from Xi'an Jiaotong University.

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Weifeng Li
    • 1
  • Ting Fan
    • 1
  • Yanmin  Zhang
    • 1
  • Xiaofeng Niu
    • 1
  • Wei Xing
    • 1
  1. 1.School of MedicineXi’an Jiaotong UniversityXi’an CityPeople’s Republic of China

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