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Inflammation

, Volume 35, Issue 3, pp 859–870 | Cite as

ICAM-1 and IL-8 Are Expressed by DEHP and Suppressed by Curcumin Through ERK and p38 MAPK in Human Umbilical Vein Endothelial Cells

  • Jia Wang
  • Sijun Dong
Article

Abstract

The present study aimed to determine whether curcumin isolated from the rhizome of Curcuma longa Linn could inhibit di-(2-ethylhexyl) phthalate (DEHP)-induced allergic inflammatory responses in human umbilical vein endothelial cells (HUVECs). We found that DEHP dose-dependently elevated adhesion molecule-1 (ICAM-1) protein level within 15–30 min, which was independent of de novo protein synthesis. And a late-phase induction of ICAM-1 was observed within 8 h treatment of DEHP via de novo protein synthesis through transcription and translation. DEHP also increased the expression of interleukin (IL)-8 in a time- and dose-dependent manner. Pretreatment with curcumin dose-dependently decreased DEHP-induced expression of ICAM-1 and IL-8 as well as phosphorylation of ERK1/2 and p38. Preincubation with ERK1/2 inhibitor (PD98059) or p38 inhibitor (SB203580) markedly blocked DEHP-stimulated activation of ICAM-1 and IL-8. We suggest that curcumin inhibits DEHP-induced expression of ICAM-1 and IL-8 through ERK and p38 MAPK signaling pathways in HUVECs and may contribute to ameliorate pathologies of DEHP-related allergic disorders.

KEY WORDS

curcumin di-(2-ethylhexyl) phthalate (DEHP) intercellular adhesion molecule-1 (ICAM-1) interleukin (IL)-8 

Notes

ACKNOWLEDGMENTS

This investigation was supported by 100 Talents Program of Chinese Academy of Sciences (KZCX2-YW-BR-18) and the Science and Technology Planning Project of Xiamen City (3502Z20112019).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Institute of Urban EnvironmentChinese Academy of SciencesXiamenPeople’s Republic of China

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