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Inflammation

, Volume 35, Issue 2, pp 605–613 | Cite as

Oxymatrine Ameliorates l-Arginine-Induced Acute Pancreatitis in Rats

  • Zhiqiang Zhang
  • Yanqing Wang
  • Ming Dong
  • Jianchun Cui
  • Daqing Rong
  • Qi Dong
Article

Abstract

The aim of this study was to determine whether oxymatrine has a protective effect against acute pancreatitis (AP) in a rat model of l-arginine-induced AP. AP was induced by two intraperitoneal injections of l-arginine (250 mg/100 g) at a 1-h interval. Oxymatrine (50 mg/kg) was administered every 6 h after the induction of AP. Oxymatrine significantly reduced the plasma amylase, d-lactic acid and tumor necrosis factor alpha concentration, serum diamine oxidase and lipase activity, and pancreatic myeloperoxidase activity, which were increased in AP rats (P < 0.05). In addition, the pancreatic CD45 expression and the expression of claudin-1, but not zonula occludens-1 (ZO-1) and occludin, in the intestinal tissues were significantly reduced after the induction of AP. However, oxymatrine increased the expression of claudin-1 and CD45, but did not alter the expression of ZO-1 and occludin. In conclusion, our results demonstrated that oxymatrine is potentially capably of protecting against l-arginine-induced AP and attenuating AP-associated intestinal barrier injury by up-regulation of claudin-1.

KEY WORDS

oxymatrine acute pancreatitis tight junction proteins tumor necrosis factor α CD45 

Notes

ACKNOWLEDGMENTS

This study was supported by grants from The National Natural Science Foundation of China (grant number 30840076) and The Natural Science Foundation of Liaoning Province (grant number 20082058).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhiqiang Zhang
    • 1
  • Yanqing Wang
    • 1
  • Ming Dong
    • 2
  • Jianchun Cui
    • 1
  • Daqing Rong
    • 1
  • Qi Dong
    • 1
  1. 1.Department of General SurgeryThe People’s Hospital of Liaoning ProvinceShenyangPeople’s Republic of China
  2. 2.Department of General SurgeryThe First Affiliated Hospital of China Medical UniversityShenyangPeople’s Republic of China

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