Oxymatrine Ameliorates l-Arginine-Induced Acute Pancreatitis in Rats
- 299 Downloads
The aim of this study was to determine whether oxymatrine has a protective effect against acute pancreatitis (AP) in a rat model of l-arginine-induced AP. AP was induced by two intraperitoneal injections of l-arginine (250 mg/100 g) at a 1-h interval. Oxymatrine (50 mg/kg) was administered every 6 h after the induction of AP. Oxymatrine significantly reduced the plasma amylase, d-lactic acid and tumor necrosis factor alpha concentration, serum diamine oxidase and lipase activity, and pancreatic myeloperoxidase activity, which were increased in AP rats (P < 0.05). In addition, the pancreatic CD45 expression and the expression of claudin-1, but not zonula occludens-1 (ZO-1) and occludin, in the intestinal tissues were significantly reduced after the induction of AP. However, oxymatrine increased the expression of claudin-1 and CD45, but did not alter the expression of ZO-1 and occludin. In conclusion, our results demonstrated that oxymatrine is potentially capably of protecting against l-arginine-induced AP and attenuating AP-associated intestinal barrier injury by up-regulation of claudin-1.
KEY WORDSoxymatrine acute pancreatitis tight junction proteins tumor necrosis factor α CD45
This study was supported by grants from The National Natural Science Foundation of China (grant number 30840076) and The Natural Science Foundation of Liaoning Province (grant number 20082058).
- 11.Genovese, T., E. Mazzon, R. Di Paola, C. Muia, C. Crisafulli, M. Menegazzi, G. Malleo, H. Suzuki, and S. Cuzzocrea. 2006. Hypericum perforatum attenuates the development of cerulein-induced acute pancreatitis in mice. Shock 25(2): 161–167. doi: 10.1097/01.shk.0000188326.82641.b7.PubMedCrossRefGoogle Scholar
- 13.Ling, J.Y., G.Y. Zhang, Z.J. Cui, and C.K. Zhang. 2007. Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography. Journal of Chromatography A 1145(1–2): 123–127. doi: 10.1016/j.chroma.2007.01.080.PubMedCrossRefGoogle Scholar
- 14.Lu, L.G., M.D. Zeng, Y.M. Mao, J.Q. Li, M.B. Wan, C.Z. Li, C.W. Chen, Q.C. Fu, J.Y. Wang, W.M. She, X. Cai, J. Ye, X.Q. Zhou, H. Wang, S.M. Wu, M.F. Tang, J.S. Zhu, W.X. Chen, and H.Q. Zhang. 2003. Oxymatrine therapy for chronic hepatitis B: a randomized double-blind and placebo-controlled multi-center trial. World Journal of Gastroenterology 9(11): 2480–2483.PubMedGoogle Scholar
- 16.Dong, Y., H. Xi, Y. Yu, Q. Wang, K. Jiang, and L. Li. 2002. Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: a study on the anti-hepatitis B virus mechanism of oxymatrine. Journal of Gastroenterology and Hepatology 17(12): 1299–1306.PubMedCrossRefGoogle Scholar
- 17.Chen, X., R. Sun, J. Hu, Z. Mo, Z. Yang, D. Liao, and N. Zhong. 2008. Attenuation of bleomycin-induced lung fibrosis by oxymatrine is associated with regulation of fibroblast proliferation and collagen production in primary culture. Basic & Clinical Pharmacology & Toxicology 103(3): 278–286. doi: 10.1111/j.1742-7843.2008.00287.x.CrossRefGoogle Scholar
- 18.Zhang, Y., B. Piao, B. Hua, W. Hou, W. Xu, X. Qi, X. Zhu, Y. Pei, and H. Lin. 2010. Oxymatrine diminishes the side population and inhibits the expression of beta-catenin in MCF-7 breast cancer cells. Medical Oncology. doi: 10.1007/s12032-010-9721-y.
- 19.Dong, X.Q., W.H. Yu, Y.Y. Hu, Z.Y. Zhang, and M. Huang. 2010. Oxymatrine reduces neuronal cell apoptosis by inhibiting Toll-like receptor 4/nuclear factor kappa-B-dependent inflammatory responses in traumatic rat brain injury. Inflammation Research. doi: 10.1007/s00011-010-0300-7.
- 21.Hong-Li, S., L. Lei, S. Lei, Z. Dan, D. De-Li, Q. Guo-Fen, L. Yan, C. Wen-Feng, and Y. Bao-Feng. 2008. Cardioprotective effects and underlying mechanisms of oxymatrine against Ischemic myocardial injuries of rats. Phytotherapy Research 22(7): 985–989. doi: 10.1002/ptr.2452.PubMedCrossRefGoogle Scholar
- 31.Norman, J.G., G.W. Fink, W. Denham, J. Yang, G. Carter, C. Sexton, J. Falkner, W.R. Gower, and M.G. Franz. 1997. Tissue-specific cytokine production during experimental acute pancreatitis. A probable mechanism for distant organ dysfunction. Digestive Diseases and Sciences 42(8): 1783–1788.PubMedCrossRefGoogle Scholar
- 40.Yasuda, T., Y. Takeyama, T. Ueda, M. Shinzeki, H. Sawa, T. Nakajima, and Y. Kuroda. 2006. Breakdown of intestinal mucosa via accelerated apoptosis increases intestinal permeability in experimental severe acute pancreatitis. The Journal of Surgical Research 135(1): 18–26. doi: 10.1016/j.jss.2006.02.050.PubMedCrossRefGoogle Scholar